Genetic transformation of Artemia franciscana by electroporation

Rodríguez-Sánchez, Alberto Constantino

Genetic transformation of Artemia franciscana by electroporation

dc.contributor.advisor	Aguilar-Yañez Jose Manuel	en_US
dc.contributor.advisor	Licona-Cassani, Cuautemoc	en_US
dc.contributor.author	Rodríguez-Sánchez, Alberto Constantino	en_US
dc.contributor.committeemember	Brunck, Marion E.G.	en_US
dc.date.accessioned	2018-06-18T15:52:58Z
dc.date.available	2018-06-18T15:52:58Z
dc.date.issued	2018-05-25
dc.description.abstract	Artemia franciscana also known as brine shrimp, and sea monkey is a halophilic crustacean used in aquaculture as living food and had a little role in research as toxicological model. Despite its easy handling and availability, Artemia barely has genetic engineering. The objective of this work is to develop a protocol for the genetic transformation of Artemia franciscana using electroporation and diapause cysts. Decapsulated cysts were electroporated with exponential decay voltage and square wave pulse, the efficiency was calculated. The effect of the plasmid DNA used, and the voltage were evaluated. Square wave pulse shows a better efficiency compared to the exponential. The concentration of DNA has no effect on the efficiency of transfection. Voltage at one level (1000 V·cm-1) had the best efficiency of transfection, but also the worst hatching. With protocol and the information generated, it will be possible to evaluate genetic regulatory elements to the future develop of a tool box for genetic manipulation.
dc.identifier.uri	http://hdl.handle.net/11285/630209
dc.language.iso	eng	en_US
dc.publisher	Instituto Tecnológico y de Estudios Superiores de Monterrey	esp
dc.rights	Open Access	en_US
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/3.0/us/	*
dc.subject.discipline	Ciencias / Sciences	en_US
dc.subject.keyword	Artemia	en_US
dc.subject.keyword	Artemia franciscana	en_US
dc.subject.keyword	Genetic manipulation	en_US
dc.subject.keyword	Electroporation	en_US
dc.title	Genetic transformation of Artemia franciscana by electroporation	en_US
dc.type	Tesis de maestría
html.description.abstract	<html> <head> <title></title> </head> <body> <p><i>Artemia franciscana</i> also known as brine shrimp, and sea monkey is a halophilic crustacean used in aquaculture as living food and had a little role in research as toxicological model. Despite its easy handling and availability, Artemia barely has genetic engineering. The objective of this work is to develop a protocol for the genetic transformation of <i>Artemia franciscana</i> using electroporation and diapause cysts. Decapsulated cysts were electroporated with exponential decay voltage and square wave pulse, the efficiency was calculated. The effect of the plasmid DNA used, and the voltage were evaluated. Square wave pulse shows a better efficiency compared to the exponential. The concentration of DNA has no effect on the efficiency of transfection. Voltage at one level (1000 V·cm-1) had the best efficiency of transfection, but also the worst hatching. With protocol and the information generated, it will be possible to evaluate genetic regulatory elements to the future develop of a tool box for genetic manipulation.</p> </body> </html>	en_US
refterms.dateFOA	2018-06-18T15:52:59Z
thesis.degree.discipline	Escuela de Ingenieria y Ciencias	en_US
thesis.degree.level	Maestro en Ciencias con Especialidad en Biotecnologia	en_US
thesis.degree.name	Maestria en Ciencias con Especialidad en Bitecnologia	en_US
thesis.degree.program	Campus Monterrey	en_US