Artículo
Permanent URI for this collectionhttps://hdl.handle.net/11285/345284
Artículo científico o editorial en una publicación periódica académica sujeto a revisión de pares. Cumple con los índices internacionales o bases de datos de amplia cobertura, como el listado del Current Contents, ISI WEB of Knowledge (http://isiknowledge.com/) e índice de revistas mexicanas de CONACYT (www.conacyt.mx/dac/revistas). Éstos indizan y resumen los artículos de revistas seleccionadas, en todas las áreas del saber.
Browse
Search Results
- An improved microscale method for extraction of phenolic acids from maize(Springer, 2017-10-10) Zavala-López, Mariana; García-Lara, Silverio;0000-0002-3463-957X; MARIANA ZAVALA LOPEZ;629540; SILVERIO GARCIA LARA;123152; Tecnologico de MonterreyAbstract Background Phenolic acids are a major group of secondary metabolites widely distributed in plants. In the case of maize, the major proportion of these metabolites occurs in the edible grain and their antioxidant activities are associated with improvements in human health. However, conventional extraction of secondary metabolites is very time consuming and generates a substantial amount of solvent waste. One approach to resolve these limitations is the use of microscale approaches, which minimize the quantity of solvents required, as well as the sample amounts and processing times. The objective of this work was to develop an improved microscale method for extraction of phenolic acids from maize and to compare it with a conventional extraction method. Results The improved microscale extraction method, coupled with an HPLC–DAD detection method, allowed identification of ferulic acid, p-coumaric acid in its free and bound form, and some diferulic acids. In its free form, p-coumaric acid ranged in content from 2.4 to 6.5 µg/g dry weight (dw) using the conventional method and 7.7 to 54.8 µg/g dw using the improved microscale method. Free ferulic acid content ranged from 2.6 to 12.9 µg/g dw for the conventional method and 16.8 to 181.7 µg/g dw for the improved microscale method. In its bound form, p-coumaric acid ranged in content from 6.0 to 30.6 µg/g dw for the traditional method and 34.4 to 138.6 µg/g dw for the improved microscale method. Bound ferulic acid ranged from 131.8 to 427.5 µg/g dw for the conventional method and 673.8 to 1702.7 µg/g dw for the improved microscale method. The coefficient of variation associated was lower for the improved microscale method than for the conventional method, thereby assuring the replicability of the process. Conclusions The improved microscale method proposed here increases the extraction power and batch capacity, while reducing the sample quantity, solvent amounts and extraction time. It also achieves a better replicability with a lower coefficient of variation than is possible with conventional extraction.
- TETX: a novel nuclear selection marker for Chlamydomonas reinhardtii transformation(Springer Open, 2015-04-15) García-Echauri, Sergio A; Cardineau, Guy A;0000-0001-5144-7660; SERGIO ANGEL GARCIA ECHAURI;209658; GUY CARDINEAU;344537Abstract Background Transformation of microalgae to obtain recombinant proteins, lipids or metabolites of economic value is of growing interest due to low costs associated with culture growth and scaling up. At present there are only three stable nuclear selection markers for the transformation of Chlamydomonas reinhardtii, which is the most commonly transformed microalgae, specifically: the aminoglycoside phosphotransferaseses aph7and aphVIII and the phleomycin resistance ble gene. As several microalgae are resistant to some of the antibiotics associated with the mentioned resistance genes, we have developed another alternative, tetX, a NADP-requiring Oxidoreductase that hydroxylates tetracycline substrates. We provide evidence that tetX can be used to obtain nuclear transformants of Chlamydomonas reinhardtii. Results We obtained nuclear transformants harbouring the tetX gene under the control of beta 2 tubulin or HSP70ARBCS2 promoters at an efficiency of transformation of 3.28 and 6.18 colony forming units/μg DNA respectively. This is the first report of a eukaryotic cell transformed using tetracycline as a selectable marker. Conclusions We developed a protocol for the nuclear transformation of Chlamydomonas reinhardtii using tetX as a selectable marker that confers stable resistance to tetracycline up to 100 μg/mL. We believe tetX can be used to transform Chlamydomonas reinhardtii chloroplasts, related microalgae and other aerobic organisms sensitive to any tetracycline antibiotic.
- Groundwater flow processes and mixing in active volcanic systems: The case of Guadalajara (Mexico)(Copernicus GmbH) HERNANDEZ ANTONIO, ARTURO; 96241; MAHLKNECHT, JURGEN; 120939; TAMEZ MELENDEZ, CAROL; 492616; RAMIREZ OROZCO, ALDO IVAN; 21956; PARRA SALDIVAR, ROBERTO; 35753; ORNELAS SOTO, NANCY EDITH; 105465; HERNANDEZ ANTONIO, ARTURO; 96241; MAHLKNECHT, JURGEN; 120939; TAMEZ MELENDEZ, CAROL; 492616; RAMIREZ OROZCO, ALDO IVAN; 21956; PARRA SALDIVAR, ROBERTO; 35753; ORNELAS SOTO, NANCY EDITH; 105465
- Special situations in inflammatory bowel disease: First Latin American consensus of the Pan American Crohn's and Colitis Organisation (PANCCO) (Second part) [Situaciones especiales en la enfermedad inflamatoria intestinal: primer consenso latinoamericano de la Pan American Crohn's and Colitis Organisation (PANCCO) (Segunda parte)](Asociacion Mexicana de Gastroenterologia) Francisco Javier Bosques Padilla
- Her-2 positive mucinous carcinoma breast cancer, case report(Elsevier Ltd) Gabriela Sofia Gomez Macias; Margarita Lilia Garza Montemayor
- Laccase-based biosensors for detection of phenolic compounds(Elsevier B.V.) Alemán Nava, Gibrán Sidney; Rodríguez Delgado, José Manuel; Parra Saldivar, Roberto; Martínez Chapa, Sergio Omar; Dieck Assad, Graciano
- Posterior segment intraocular foreign body: Extraction surgical techniques, timing, and indications for vitrectomy(Hindawi Publishing Corporation) Patricio José Rodriguez Valdés
- Ideal cardiovascular health predicts lower risk of abnormal liver enzymes levels in the Chilean National Health Survey (2009–2010)(Public Library of Science) Antonio García-Hermoso
- Clustering of rainfall stations in RH-24 Mexico region using the hurst exponent in semivariograms(Hindawi Publishing Corporation) Víctor Gustavo Tercero Gómez