Ciencias Exactas y Ciencias de la Salud
Permanent URI for this collectionhttps://hdl.handle.net/11285/551014
Pertenecen a esta colección Tesis y Trabajos de grado de los Doctorados correspondientes a las Escuelas de Ingeniería y Ciencias así como a Medicina y Ciencias de la Salud.
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- Micro and nanoscale alternatives for algal biotechnological developments in health and environmental sciences(Instituto Tecnológico y de Estudios Superiores de Monterrey, 2024) Silva Núñez, Andrea Arisbe; González Valdez, José Guillermo; emimmayorquin; Hernández Pérez, Jesús; Benavides Lozano, Jorge Alejandro; Santacruz López, Yolanda Arlette; Zaruma Arias, Pablo Esteban; School of Engineering and Sciences; Campus Monterrey; Ostenfeld Larsen, ThomasIn the last few years, microalgae research and development has attracted attention due to the ability of these microorganisms to produce a wide range of high added-value products. However, more research is still required to fully exploit their potential. This dissertation explores different microalgae biotechnological applications in two distinct areas, health and the environment, reason why this work is divided into two main parts that will be covered in Chapters 2 and 3. First, a study was performed to evaluate the potential of polymeric microencapsulation in the stabilization of phycoerythrin, a pigment with anticancer capacity but with stability problems that limit their potential as a pharmaceutical. In the second part, an analysis of the extracellular vesicles of three different strains of the microalga Prymnesium parvum, a species known to be involved in algal blooms worldwide, was performed. In this study, a proteomic analysis of the extracellular vesicles obtained from the three strains was conducted to compare their composition under normal, nitrogen-deficient, and phosphorus-deficient growth conditions. This analysis allowed a better understanding of the role that these vesicles play in algal blooms and how nutrient deficiency alters their composition. Considering both study frameworks, this work aims to contribute to our understanding of the potential biotechnological applications that microalgae could provide in different areas, such as health and the environment. By progressing this knowledge, we also open novel and interesting possibilities in the research and development of innovative biomedical applications to some of the ailments and diseases that to date are still uncurable. Furthermore, to better understand the role these microorganisms play in algal blooms, we can elaborate and take effective control measures to mitigate the health, environmental and economic impacts associated with these phenomena. By generating this knowledge, we are potentially contributing to the advancement of marine science and constructing foundations for future research and technological applications in the field of microalgal biotechnology.
- Novel PEGylation and purification strategies for the development of biopharmaceutical products(Instituto Tecnológico y de Estudios Superiores de Monterrey, 2021-08) Campos García, Víctor Raúl; CAMPOS GARCIA, VICTOR RAUL; 529594; González Valdez, José Guillermo; emipsanchez; Pérez Ramírez, Néstor Octavio; Martínez Calderón, Luz María; Gradilla Hernández, Misael Sebastián; Escuela de Ingeniería y Ciencias; Campus Monterrey; Benavides Lozano, Jorge AlejandroThis work presents alternative and novel PEGylation reaction and purification strategies to develop biopharmaceutical products using three PEGylated proteins as models: PEGfilgrastim, PEGRNase A and PEGlysozyme. These strategies are presented as improvements of traditional PEGylation reactions schemes that involve sequential reaction and purification steps. The present dissertation is divided in five chapters. The first chapter serves as an introduction of the topic giving the general background regarding PEGylation as a technique to develop long- acting therapeutics and the methodologies used for its analysis. The results of the experiments herein proposed are presented in Chapter 2, Chapter 3 and Chapter 4, included in published or submitted scientific papers. The general conclusions of the dissertation are presented in Chapter 5, including perspectives for further studies in the same work line.