Ciencias Exactas y Ciencias de la Salud
Permanent URI for this collectionhttps://hdl.handle.net/11285/551039
Pertenecen a esta colección Tesis y Trabajos de grado de las Maestrías correspondientes a las Escuelas de Ingeniería y Ciencias así como a Medicina y Ciencias de la Salud.
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- Comparación de niveles de microRNAs circulantes entre pacientes mexicanos con esclerosis lateral amiotrófica y controles sanos(Instituto Tecnológico y de Estudios Superiores de Monterrey, 2025-05-27) Ledezma Ramírez, Arturo; Cuevas Díaz Durán, Raquel; emimmayorquin, emipsanchez; Martínez Ledesma, Juan Emmanuel; García Magariño Alonso, Mariano; Souza, Carolina; Escuela de Medicina y Ciencias de la Salud; Campus MonterreyLa esclerosis lateral amiotrófica (ELA) es una enfermedad neurodegenerativa caracterizada por muerte de neuronas motoras y pérdida de funciones motoras voluntarias e involuntarias. Los microRNAs (miRNAs) son pequeñas secuencias de RNAs no codificantes que han demostrado tener un papel importante en la regulación y el silenciamiento de genes posterior a su transcripción. Se ha observado la participación de miRNAs desregulados en enfermedades crónico-degenerativas y neurodegenerativas, entre ellas la ELA, como mediadores de los procesos fisiopatológicos. Estudios recientes han determinado que los niveles de miRNAs circulantes y su expresión pueden ser candidatos prometedores para biomarcadores en la ELA. El presente es un estudio piloto clínico, observacional, transversal y analítico que busca conocer si los niveles de miRNAs circulantes son significativamente distintos entre pacientes con ELA y controles neurológicamente sanos. Usando el kit de extracción miRNEAsy de Qiagen y un fluorómetro Qubit 3.0 se lograron extraer miRNAs de muestras de plasma de pacientes con ELA y controles. Los análisis de resultados demostraron pocas diferencias significativas entre los grupos, pero se observó una ligera correlación positiva entre niveles de miRNAs y edad avanzada. Las futuras direcciones en esta línea de investigación deben ir orientadas a confirmar estos hallazgos aumentando el tamaño de la población de estudio.
- Anticancer activity of Elaphoglossum paleaceum (Hook. & Grev.) sledge derived phloroglucinols in human hepatocellular carcinoma cells through potential transcriptional modulation of microRNAs(Instituto Tecnológico y de Estudios Superiores de Monterrey, 2022-01-05) Ruiz Manriquez, Luis Mario; PAUL, SUJAY; 512948; Paul, Sujay; emijzarate/puemcuervo; Arvizu Espinosa, María Goretti; School of Engineering and Sciences; Campus Monterrey; Sharma, AshutoshPhloroglucinols derivatives are an important class of secondary metabolites widely distributed in Pteridophytes of the genera Elaphoglossum that exhibit multiple biological activities, including anticancer activity. Recent evidence suggests that many phytochemicals, including polyphenols, can significantly alter microRNA (miRNAs) expression profiles. miRNAs are short non-coding RNAs that can act as tumor suppressors or onco-miRNAs regulating the expression of cancer-associated genes post-transcriptionally, therefore, modulating cancer progression. Elaphoglossum paleaceum (Hook. & Grev.) Sledge phloroglucinols derivatives have demonstrated cytotoxic activity against several cancer models. Nevertheless, its relative mechanism of action and its effect on HCC cells have not still investigated. The present study aimed to evaluate the anticancer activity of hexanic/chloroformic extract of E. paleaceum and investigate its mechanism(s) of action on tumor suppressor and onco-miRNAs expression involved in HCC carcinogenesis. E. paleaceum hexanic/chloroformic extract exhibited cytotoxicity against HepG2 cells. Moreover, an E. paleaceum hexanic/chloroformic extract concentration approximating the IC50 induced upregulation of tumor suppressors miR-200b-5p, miR-34-5p, Let-7b-5p, and miR-218b-5p. These modulatory effects might contribute to inhibiting HepG2 cell growth observed after treatment with E. paleaceum hexanic/chloroformic extract. These data demonstrate the ability of E. paleaceum phloroglucinols derivatives to impede hepatocellular carcinoma cell proliferation, possibly by modulating critical miRNAs and consequently their downstream targets, suggesting a novel mechanism of the anticancer potential of phloroglucinols derivatives and positioning them as potential novel anticancer agents.
- Metabolomic Profiling, miRNAs Analysis and Molecular Docking of Two Different Populations of the Genus Galphimia(Instituto Tecnológico y de Estudios Superiores de Monterrey, 2020-06-15) Sanchez Garza, Betsabe; SHARMA, ASHUTOSH; 398262; Sharma, Ashutosh; RR; Paul, Sujay; Escuela de Ingeniería y Ciencias; Campus Querétaro; Angulo Bejarano, Paola IsabelGalphimia glauca (Cav.) Kuntze is medicinal plant from Mexican origin that belongs to the Malpighiaceae family, important in traditional medicine for its popular use in treating disorders of the Central Nervous System (CNS) such as anxiety. Geographically, the 22 species of Galphimia spp. are widely distributed all over Mexico and G. glauca, is distributed in 8 states. Plants respond to their environment by generating different phytochemicals to withstand both biotic and abiotic stress and, previous studies demonstrated that population from Guanajuato has been identified as producing the main compound with anxiolytic activity, Galphimine B that belongs to a group of nor-seco triterpenes: galphimines (A-I); while a population of San Luis Potosí shows an absence of these compounds. In the present study, a differential phytochemical profile of the compounds present in producer (P) and non-producer (NP) populations from the Galphimia genus, was obtained. As a result, the P population exhibited a higher amount of triterpene compounds compared to the NP population, which presented higher number of polyphenol compounds, as revealed by the gas chromatography-mass spectrometry (GC-MS) analysis. Multivariate analysis from GC-MS data demonstrated two different clusters of compounds, according to their level of correlations between chemical classes, supporting the previous categorization of P and NP. The confirmatory analysis done by high-performance liquid chromatography (HPLC), corroborated this categorization, where the P samples pesented the characteristic galphimine peaks, which were not present in NP samples. Further metabolomic analysis done by ultra-high-performance liquid chromatography-spectrometry of mass (UHPLC-QTOF-MS), complemented the information obtained by the GC-MS analysis, and revealed two compounds involved in the triterpenoid synthesis. To understand the genetic regulation in both populations, a miRNAs analysis was performed. As a result, 2 possible miRNAs were obsereved expressing higher levels in P producer in relation with NP population, targeting gene expression [Terpene synthases (TPSs) and Farnesyl Diphosphate synthases (FDS)] for the triterpenoid biosynthetic pathways. The results obtained from the metabolomic analysis were utilized for in silico prediction of chemical-medicinal mechanisms from P population, in which tree phytochemical compounds from the P population (β-sitosterol, Vitamin E and Galphimine B) were predicted to have a fair affinity for their respective target proteins assed by molecular docking, the obtained results will serve to the understanding of the effects on CNS and anti-inflammatory response. The integrated information in the present study would serve for further validation of phytochemical compounds, as well as a basis for the future studies in Galphimia glauca such as the relation between transcriptome and metabolomic profiles by using the provided miRNAs regulation information as a scaffold, with future application in with in the design of new biotechnological strategies to exploit this plant.