Ciencias Exactas y Ciencias de la Salud
Permanent URI for this collectionhttps://hdl.handle.net/11285/551039
Pertenecen a esta colección Tesis y Trabajos de grado de las Maestrías correspondientes a las Escuelas de Ingeniería y Ciencias así como a Medicina y Ciencias de la Salud.
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- Engineered mesoporous silica nanoparticles for the co-delivery of quercetin and resveratrol: structural characterization and assessment of antioxidant and anti-inflammatory potential(Instituto Tecnológico y de Estudios Superiores de Monterrey, 2025-12-02) Torres Copado, Andrea; Paul, Sujay; mtyahinojosa, emipsanchez; Arvizu Espinosa, María Goretti; Sahare, Padmavati; School of Engineering and Sciences; Campus Monterrey; Estévez González, Miriam RocíoThe global burden of noncommunicable diseases (NCDs) is closely associated with persistent oxidative stress and chronic inflammation. Natural polyphenols such as quercetin and resveratrol possess potent antioxidant and anti-inflammatory activities; however, their therapeutic potential is severely hindered by low aqueous solubility, poor chemical stability, and rapid metabolic degradation. Nanotechnology-based delivery systems offer a promising approach to enhance the bioavailability and functional performance of these bioactive compounds. Accordingly, this work aimed to co-encapsulate quercetin and resveratrol into mesoporous silica nanoparticles (MSNs), thoroughly characterize the resulting nanocarrier system, and assess its biological properties in vitro. MSNs were synthesized through a modified Stöber method, yielding uniform, spherical, amorphous nanoparticles with an average hydrodynamic diameter of ~126 nm, a high specific surface area (200.3 m²/g), a pore volume of 0.445 cm³/g, and a mean pore diameter of 5.4 nm. Co-loading was achieved using a solvent evaporation method, resulting in high encapsulation efficiencies (79.9% for quercetin and 71.4% for resveratrol). Physicochemical characterization (FTIR, XRD, TGA, DLS, Zeta Potential) confirmed successful drug incorporation, partial amorphization of the polyphenols, enhanced thermal stability, and a sustained release profile extending to 75 hours. The QUE-RES-SiO₂ formulation demonstrated significantly enhanced antioxidant capacity in DPPH, CUPRAC, and ABTS assays, surpassing free resveratrol. Strong anti-inflammatory capacity was also observed in a heat-induced protein denaturation model, with up to 75% inhibition, comparable to free quercetin and the reference drug diclofenac. In ovarian adenocarcinoma SKOV-3 cells, the formulation exhibited efficient nanoparticle uptake; however, it did not induce cytotoxicity or reactive oxygen species (ROS) production within 24 hours, likely due to slow-release kinetics, intrinsic chemoresistance of the cell line, and low concentrations tested over a limited time. Overall, these results demonstrate that MSNs constitute an effective platform for the co-delivery of quercetin and resveratrol, enhancing their stability and antioxidant and anti-inflammatory potential while overcoming key physicochemical limitations. Although anticancer effects were not observed under the tested conditions, this study establishes a robust foundation for future optimization of release kinetics, dosing strategies, and targeting mechanisms to exploit the therapeutic potential of polyphenols in oxidative stress- and inflammation-driven chronic diseases.
- Design of nanoparticles composed by epicatechin and quercetin to modify bioavailability.(Instituto Tecnológico y de Estudios Superiores de Monterrey, 2022) Ayala Fuentes, Jocelyn Concepción; CHAVEZ SANTOSCOY, ROCIO ALEJANDRA; 290063; Chávez Santoscoy, Rocío Alejandra; puelquio/mscuervo; Serna Saldívar, Sergio O.; Antunes Ricardo, Marilena; School of Engineering and Sciences; Campus MonterreyFlavonoids are valuable compounds present in natures with many health benefits such as anti-oxidative, anti-inflammatory, anti-mutagenic, and anti-carcinogenic. Among the flavonoids, epicatechin and quercetin gained particular attention for their potential therapeutic activities in noncommunicable chronic diseases (NCDs). Unfortunately, these flavonoids show lack long-term stability, sensitive to light, present low water solubility and poor bioavailability. In recent decades, Nanotechnology has been a promising strategy to enhance flavonoids' benefits through on the compound’s delivery to various sites within the body due to its size. The following thesis has as aims to design a novel inulin-soy protein nanoparticles (NEQs), which simultaneously loaded epicatechin and quercetin, to improve their stability, bioavailability, and prevention of NCDs. Process optimization, nanoparticle characterization, in vitro release, in vitro gastrointestinal digestion, and cytotoxicity effect of NEQs were investigated. Results showed that NEQs are average size of 280.17 ± 13.42 nm and a zeta potential of -18.267 ± 0.83 mV in organic phase. Entrapment efficiency of epicatechin and quercetin reached 97.04 ± 0.01 and 92.05 ± 1.95 %, respectively. The used of soy protein improve the stability in gastrointestinal conditions. Also, NEQs presented effects of inhibition in Caco-2, but not in HepG-2 and HDFa cells. These results contribute basis to the design and fabrication of inulin-soy protein nanoparticles as a delivery system for multiple bioactive compounds with beneficial properties.