Ciencias Exactas y Ciencias de la Salud
Permanent URI for this collectionhttps://hdl.handle.net/11285/551039
Pertenecen a esta colección Tesis y Trabajos de grado de las Maestrías correspondientes a las Escuelas de Ingeniería y Ciencias así como a Medicina y Ciencias de la Salud.
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- Evaluation of the biological activity of wounding stress-treated carrots on the development of obesity and associated metabolic disorders(Instituto Tecnológico y de Estudios Superiores de Monterrey, 2024-12-11) Castorena Ramírez, Mariana Denise; Jacobo Velázquez, Daniel Alberto; emimmayorquin; Chuck Hernández, Cristina Elizabeth; Licona Cassani, Cuauhtémoc; School of Engineering and Sciences; Campus Monterrey; Rabadán Chávez, Griselda MericiaAdipose tissue is a complex multicellular organ that serves as both an energy reservoir and an endocrine organ responsible for maintaining energy homeostasis through a set of integrated endocrine and metabolic responses. By storing and mobilizing energy as needed, adipose tissue supports metabolic balance. Similarly, the gut microbiota—a complex intestinal microbial ecosystem—plays a critical role in metabolic health, impacting obesity through lipid metabolism, energy extraction, and inflammation modulation. Postharvest wounding stress, a type of abiotic stress induced by cutting followed by a storage period, has been shown to significantly increase the total phenolic content in carrots, particularly chlorogenic acid (CHA). Given the antioxidant, anti-inflammatory, and anti-obesogenic effects of this dietary phytochemical, this study aimed to evaluate the effect of consuming carrots treated with wounding stress on diet-induced obesity (DIO) and associated metabolic disorders in rats. Male Wistar rats were fed a standard (SD) or hypercaloric diet (HD) supplemented with wounding stress-treated carrots (wsC) or nonstressed carrots (nsC) for 8 weeks. In HD-fed rats, daily consumption of 5 g of wsC (HDwsC) led to a significant decrease in body weight gain (18%) and total white adipose tissue (WAT) accumulation (9.7%) without changes in food or energy intake compared to the HD group. HD-wsC supplementation also improved fat mass distribution, with a significant increase in subcutaneous WAT (20%) and a decrease in visceral WAT (17.3%). These shifts in adipose tissue were consistent with improvements in lipid profiles, as the HD-wsC group showed increased HDL-c levels (40%) and reduced triglycerides (39%), total cholesterol (35%), LDL-c (8.3%), and VLDL-c (38.6%). Additionally, HD-wsC improved glucose metabolism, enhancing oral glucose tolerance and insulin sensitivity, as reflected in lowered AUC values (4.3% and 5.2%, respectively, vs. HD group). Microbiota analysis further revealed that HD-wsC partially alleviated gut dysbiosis, preserving microbial diversity, increasing beneficial bacteria like Butyricoccaceae, and reducing pathogenic bacteria such as Rickettsiaceae. These findings suggest that wounding stress treated-carrots may offer an effective dietary intervention for obesity management, acting through modulation of adipose tissue function, lipid profiles, and gut microbiota composition.
- Analysis of immune cells in peripheral blood and colostrum from obese mothers in Mexico(Instituto Tecnológico y de Estudios Superiores de Monterrey, 2021-05-28) Piñeiro Salvador, Raúl; Brunck, Marion; tolmquevedo; Lara-Díaz, Víctor Javier; Licona-Cassani, Cuauhtémoc; Vásquez-Garza, Eduardo; Escuela de Ingeniería y Ciencias; Campus MonterreyBreastfeeding provides newborns not only nutritional short-term nutrient supply, but also long-term benefits in neural, metabolic, and immune development. Several bioactive components of human milk mediate these long-term benefits over immunity, including living bacteria and leukocytes, or even some modulators like cytokines and antibodies. The immunological properties of milk change over lactation progress and the colostrum (stage of lactation produced within 2 days after birth) is the stage with the highest concentration of these factors. Other factors like maternal health status, genetics or daily variations also influence on milk composition. In this scenario, prolactin promotes leukocyte migration to mammary glands and milk. However, the role of chronic conditions on human milk immune composition like obesity is poorly studied. This is especially important in countries with high rates of maternal obesity, like Mexico, where more than two-thirds of adult women suffer any grade of overweight or obesity. Here, we present an extensive flow-cytometry based characterization of leukocyte subpopulations in peripheral blood and colostrum from lean and obese mothers, as well as an analysis of some of their phenotypes. With this data, we aim to better understanding if obesity affects the process of leukocyte migration to human milk, and promotes possible changes on functionality of human milk leukocytes.
- Effect of moderate exercise on skeletal muscle mitochondrial function and dynamics in obese Zucker rats(Instituto Tecnológico y de Estudios Superiores de Monterrey, 2020-06) Nieblas León, Bianca Daniela; NIEBLAS LEON, BIANCA DANIELA; 450250; García Ramírez, Noemí; RR, emipsanchez; García Rivas, Gerardo de Jesús; Castorena Torres, Fabiola; Hernández Brenes, Carmen; De la Garza Hernández, Ana Laura Isabel; Escuela de Medicina y Ciencias de la Salud; Campus Monterrey; Altamirano Barrera, JulioObesity and metabolic syndrome are related to a significant reduction in mitochondrial quality. Current evidence suggests that both conditions promote an unbalance between fusion and fission, the main events of mitochondrial dynamics and this is associated with alterations in mitochondrial function, mainly in highly energetic tissues such as skeletal muscle. In the treatment of obesity and metabolic disorders, the various adaptations resulting from moderate-intensity physical exercise stand out, which are considered to be regulated by AMPK. This project aimed to evaluate modifications in gene and protein expression of the main regulators of mitochondrial dynamics and, using confocal microscopy, determine changes in mitochondrial function through the evaluation of membrane potential and distribution changes in the main mitochondrial subpopulations in a murine model of obesity after chronic moderate exercise. 12 weeks old male Zucker obese rats were randomly divided into a sedentary obese group and an exercise obese group (n=4 per group). The exercise consisted of 4 weeks of swimming training for 60min/5days a week. After 48 hours of the last exercise bout, animals were euthanized and both gastrocnemius muscles were isolated. A significant increase in fission was evidenced by changes in phosphorylation of Drp1 and AMPK. Subsarcolemmal mitochondria showed a more organized network in comparison with the sedentary group while there was no change in the intermyofibrillar region. An increase in fission regulated by AMPK might be segregating damaged mitochondria and enhancing its removal while activating mitochondrial biogenesis to ensure restoration of mitochondrial mass by generating a healthier population in the subsarcolemmal region.
