Ciencias Exactas y Ciencias de la Salud
Permanent URI for this collectionhttps://hdl.handle.net/11285/551039
Pertenecen a esta colección Tesis y Trabajos de grado de las Maestrías correspondientes a las Escuelas de Ingeniería y Ciencias así como a Medicina y Ciencias de la Salud.
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- PVA /alginate/AgNPs/curcumin electrospun nanofibers-based nonwoven mat as wound dressing(Instituto Tecnológico y de Estudios Superiores de Monterrey, 2025-06-13) Rodríguez Lee, Fernando Arturo; García López, Erika; emipsanchez; Rodríguez González, Ciro Ángel; Tejeda Alejandre, Raquel; School of Engineering and Sciences; Campus Monterrey; Vázquez Lepe, Elisa VirginiaWound healing requires advanced solutions that can support tissue regeneration, reduce inflammation, and prevent infections. This thesis presents the development of an electrospun wound dressing made from polyvinyl alcohol (PVA), alginate, silver nanoparticles (AgNPs), and curcumin. The objective was to create a multifunctional dressing capable of improving healing in burn wounds by combining the mechanical stability of PVA, the moisture retention of alginate, and the antimicrobial and anti-inflammatory properties of AgNPs and curcumin. Rheological tests were performed to identify critical polymer concentrations and determine the influence of molecular weight on fiber morphology. Electrospinning conditions were optimized to obtain bead-free nanofibers with diameters ranging from 200 to 600 nm. Scanning electron microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDS) confirmed the presence and distribution of active agents in the fibers. Biological evaluation using fibroblast 3T3 cells under direct contact conditions demonstrated good cell compatibility, especially in the first three days. It is important to highlight that by day seven, the culture medium was not replaced, yet cells still showed metabolic activity despite the reduced nutrients and suboptimal pH. This result suggests that the material provides a suitable environment that supports cell viability over time. The results show that this electrospun dressing has potential for use in burn wound healing applications.
- Evaluation of the Synergistic Effects of Curcumin-Resveratrol co-loaded Mesoporous Silica Nanoparticles on Colorectal Cancer Cells(Instituto Tecnológico y de Estudios Superiores de Monterrey, 2023-12-04) Ochoa Sánchez, Adriana; Paul, Sujay; emimmayorquin; Arvizu Aguilar, María Goretti; EIC Escuela de Ingeniería y Ciencias; Campus Monterrey; Estévez González, Miriam RocíoColorectal cancer (CRC) poses a substantial global health challenge, necessitating innovative solutions due to the limitations inherent in current conventional treatments. Curcumin and resveratrol, natural phytochemicals, have emerged as promising anticancer agents, offering therapeutic benefits with minimal toxicity to healthy tissues. However, the clinical application of these phytochemicals faces limitations. Nanoformulations have gained prominence for their ability to overcome these constraints, ensuring improved solubility, controlled release, and enhanced biocompatibility. Mesoporous silica nanoparticles (MSNs) have garnered attention as nanocarriers due to their exceptional biocompatibility and high load capacity. This study focused on investigating the effect of co-loaded curcumin and resveratrol MSNs on target gene expression, which is crucial in cancerogenesis. Curcumin and resveratrol were immobilized onto MSNs functionalized with 3- aminopropyltriethoxysilane (APTES) and glutaraldehyde (GTA). The co-loaded MSNs were characterized using FTIR, TGA, SEM, XRD, BET, and determination of encapsulation efficiency by UV-Visible spectrophotometry. In vitro cytotoxicity assays were conducted using HCT-116 and Caco-2 colorectal cell lines. Target gene expression was evaluated through RT-qPCR after treating HCT-116 cancer cells with the nanoformulation at 24 and 48-hour time points. The results revealed significant upregulation of the tumor suppressor gene TP53 and apoptosis-related gene Bax, coupled with downregulation of proliferation-related genes Wnt-1 and CTNNB1. These findings demonstrate the ability of co-loaded curcumin and resveratrol MSNs to modulate key genes involved in cancer progression, showcasing their potential as a promising therapeutic strategy for CRC.