Ciencias Exactas y Ciencias de la Salud
Permanent URI for this collectionhttps://hdl.handle.net/11285/551039
Pertenecen a esta colección Tesis y Trabajos de grado de las Maestrías correspondientes a las Escuelas de Ingeniería y Ciencias así como a Medicina y Ciencias de la Salud.
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- Biofabrication of anisotropic constructs using extrusive chaotic printing(Instituto Tecnológico y de Estudios Superiores de Monterrey, 2023-12-01) Hernandez Medina, David Hyram; Trujillo de Santiago, Grissel; mtyahinojosa, emipsanchez; Bolívar Monsalve, Edna Johana; Castillo, Jimmy; Esquivel Alfaro, Marianelly; Escuela de Ingeniería y Ciencias; Campus Monterrey; Álvarez, Mario MoisésAligned tissue constitutes a considerable percentage of the body mass, and it is this anisotropic characteristic which confers certain mechanical and functional properties to the tissue. For creating tissue-like structures that resembles the body, one relevant challenge lies on using biomaterials and shaping them to create aligned structures. These constructs serve as scaffolding materials that promote cell proliferation and differentiation that can eventually become a working tissue. In this study, continuous chaotic printing was used to fabricate highly oriented printed scaffolds and bioprinted cell-laden constructs. First, we assessed the effectiveness of a chaotic extrusion printhead, containing a Kenics Static Mixer (KSM), as a tool to align fibrillar inks. In short, soft fibrillar materials (i.e., alginate-cellulose and collagen) were chaotically printed into 1 mm thick filaments and scaffold anisotropy was assessed in terms of printed microstructures orientation. Filaments showed orientation up to 68% in a -15° to 15° region where the main axis (i.e., aligned fiber) correspond to 0°. Moreover, we assessed the capability of chaotic bioprinting to produce aligned and pre-vascularized skeletal-muscle-like tissues. Briefly, fibers were bioprinted using three inks: a hydrogel loaded with myoblasts (C2C12 cells), a non-crosslinkable hydrogel to create inner vessels inside the fiber, and a high viscosity hydrogel loaded with mesoporous bioactive glass (MBG) to provide mechanical robustness to the fiber. A comparison was made between homogeneous (pre mixed) fibers and pre-vascularized fibers with a layered inner structure. The constructs were cultured up to 21 days and demonstrated high viability (>85%) and a significant relation in the orientation trend of the F-actin filaments with the stratification. Overall, we demonstrated that chaotic printing is a practical tool for fabricating anisotropic constructs with both, fibrillar inks and cell-laden constructs.
- Biofabrication of nanoenhanced hydrogel fibers for muscle tissue engineering using surface chaotic flows: Chaotic 2D-printing(Instituto Tecnológico y de Estudios Superiores de Monterrey, 2020) Frías Sánchez, Ada Itzel; TRUJILLO DE SANTIAGO, GRISSEL; 256730; FRIAS SANCHEZ, ADA ITZEL; 887018; ALVAREZ, MARIO MOISES; 26048; Trujillo de Santiago, Grissel; RR; Tamayol, Ali; Ponz Ascaso, Fernando; Samandari, Mohamadmahdi; School of Engineering and Sciences; Campus Monterrey; Alvarez, Mario MoisésMultiple human tissues exhibit a fibrous nature. Therefore, the fabrication of hydrogel filaments for biomedical engineering applications is a trending topic. Current tissue models are made of materials that often require further enhancement for appropriate cell attachment, proliferation and differentiation. Here we present a simple strategy, based on the use of mathematically modelable surface chaotic flows, to fabricate continuous, long and thin filaments of gelatin methacryloyl (GelMA) added with Turnip mosaic virus (TuMV) for enhanced muscle tissue engineering. The fabrication of these filaments was achieved by chaotic advection in a finely controlled and miniaturized version of the journal bearing (JB) system. A drop of a pre-gel solution of GelMA was injected on a higher-density viscous fluid (glycerin) and a chaotic flow was applied through an iterative process. The hydrogel drop exponentially deformed and elongated to generate a fiber, which was then photocrosslinked under exposure to UV light. Computational fluid dynamics (CFD) simulations were conducted for the design and prediction of our results. GelMA fibers were then used as scaffolds for C2C12 myoblast cells, and the effect of adding plant-based viral nanoparticles (VNP) to the hydrogel fibers as nano-scaffolds for cellular growth was evaluated. Chaotic 2D-printing was proven to be a viable method for the fabrication of hydrogel fibers. CFD simulations accurately predicted the lengths of the printed fibers, and a correlation coefficient of R2=0.9289 was determined from the experimental and simulated data of the first two cycles. The hydrogel fibers were effective scaffolds for muscle cells and show potential to be used as cost-effective models for muscle tissue engineering purposes. TuMV significantly increased the metabolic activity of the cell-seeded fibers (p<0.05), strengthened cell attachment throughout the first 28 days, improved cell alignment to ~50%, and promoted the generation of structures that resemble natural mammal muscle tissue.