Ciencias Exactas y Ciencias de la Salud
Permanent URI for this collectionhttps://hdl.handle.net/11285/551039
Pertenecen a esta colección Tesis y Trabajos de grado de las Maestrías correspondientes a las Escuelas de Ingeniería y Ciencias así como a Medicina y Ciencias de la Salud.
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- Synthesis and obtention of CaSiO3 and WO3 ceramic particles as reinforcing fillers of Poly(vinyl alcohol)/Gelatin hydrogels for cartilage regeneration(Instituto Tecnológico y de Estudios Superiores de Monterrey, 2022-06-01) Catzim Ríos, Kevin Stalin; ORTEGA LARA, WENDY DE LOURDES; 95211; Ortega Lara, Wendy de Lourdes; puelquio, emipsanchez; Melo Máximo, Dulce Viridiana; López Mena, Edgar René; Soria Hernández, Cintya Geovanna; School of Engineering and Sciences; Campus Monterrey; Soria Hernández, Cintya GeovannaCartilage wear is a problem that affects a large percentage of the population and has gained relevance in recent years. However, current treatments do not present optimal results that favor the quality of life of those affected. Research in this field has recently focused on the development of systems that promote tissue regeneration instead of replacing it. In this work, the synthesis of CaSiO3 and WO3 ceramic nanoparticles was studied using chemical methods such as sol-gel and precipitation respectively, to later be used as reinforcement of hydrogels composed of poly(vinyl-alcohol)/Gelatin (PVA/Gel) for the improvement of hydrogel bioactivity within biological systems. For the CaSiO3 synthesis, a single pure crystalline phase was obtained, with average particle sizes between 40 and 150 nm. On the other hand, for the WO3 particles, average sizes of 130 nm were obtained. Both independent nanoparticle syntheses were characterized by XRD, SEM, FTIR, DLS and EDX. Viability assays revealed that the hydrogel formulation lowers cell viability by at least 50% in fibroblasts (NIH) and osteoblasts (HFOB). However, silicon-rich particles were found to help improve viability, promoting cell proliferation. Finally, a new non-commercial printing system was developed for freeze-thaw crosslinked hydrogels, where the possibility of 3D printing the generated PVA/Gel formulation was verified.
- Evaluation of in vitro antioxidant and antibacterial activities and hydrolysates from Sphenarium purpurascens of early and adult stage(Instituto Tecnológico y de Estudios Superiores de Monterrey, 2021-11-01) Marín Morales, Martha Selene; IBARRA HERRERA, CELESTE CONCEPCION; 265151; Ibarra Herrera, Celeste Concepción; emijzarate/puemcuervo; Pérez Carrillo, Esther; Amador Espejo, Genaro Gustavo; Luna Vital, Diego Armando; School of Engineering and Sciences; Campus MonterreyEdible insects have become a promising food source since it has been shown that they are rich in protein, fatty acids, minerals, among others. Sphenarium purpursacens is considered an edible insect endemic from Mexican culture and its geographical distribution includes Tlaxcala, Puebla, Guanajuato, Queretaro, Hidalgo y México. Furthermore, grasshoppers contain several nutritional compounds in which the percentage of protein stands out. Obtaining innovative and alternative ingredients with biological activity from edible insect proteins has increased the demand for which it is necessary to advance research regarding entomophagy. The present study aimed was determined the antioxidant properties of the extract obtained from Sphenarium purpurascens early and adult age and evaluate the differences between them. It was carried out analyses compared proximal analyses of different stages of development of the insect and have been reported slightly different which are attributed to the age of the grasshoppers. Besides, the application of enzymatic hydrolysis using the Bacillus licheniformis 2.4L protease caused an increase in antioxidant capacity against DPPH• and ABTS•+. Once fractionated by ultrafiltration, the fractions that presented the highest antioxidant activity against DPPH• and ABTS•+ were ≤10 kDa. In conclusion, the enzymatic treatment with endoproteases proved to be an effective way to improve the concentration of compounds from the grasshopper, thus generating a viable source of possible nutraceuticals.