Ciencias Exactas y Ciencias de la Salud
Permanent URI for this collectionhttps://hdl.handle.net/11285/551039
Pertenecen a esta colección Tesis y Trabajos de grado de las Maestrías correspondientes a las Escuelas de Ingeniería y Ciencias así como a Medicina y Ciencias de la Salud.
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- Engineered mesoporous silica nanoparticles for the co-delivery of quercetin and resveratrol: structural characterization and assessment of antioxidant and anti-inflammatory potential(Instituto Tecnológico y de Estudios Superiores de Monterrey, 2025-12-02) Torres Copado, Andrea; Paul, Sujay; mtyahinojosa, emipsanchez; Arvizu Espinosa, María Goretti; Sahare, Padmavati; School of Engineering and Sciences; Campus Monterrey; Estévez González, Miriam RocíoThe global burden of noncommunicable diseases (NCDs) is closely associated with persistent oxidative stress and chronic inflammation. Natural polyphenols such as quercetin and resveratrol possess potent antioxidant and anti-inflammatory activities; however, their therapeutic potential is severely hindered by low aqueous solubility, poor chemical stability, and rapid metabolic degradation. Nanotechnology-based delivery systems offer a promising approach to enhance the bioavailability and functional performance of these bioactive compounds. Accordingly, this work aimed to co-encapsulate quercetin and resveratrol into mesoporous silica nanoparticles (MSNs), thoroughly characterize the resulting nanocarrier system, and assess its biological properties in vitro. MSNs were synthesized through a modified Stöber method, yielding uniform, spherical, amorphous nanoparticles with an average hydrodynamic diameter of ~126 nm, a high specific surface area (200.3 m²/g), a pore volume of 0.445 cm³/g, and a mean pore diameter of 5.4 nm. Co-loading was achieved using a solvent evaporation method, resulting in high encapsulation efficiencies (79.9% for quercetin and 71.4% for resveratrol). Physicochemical characterization (FTIR, XRD, TGA, DLS, Zeta Potential) confirmed successful drug incorporation, partial amorphization of the polyphenols, enhanced thermal stability, and a sustained release profile extending to 75 hours. The QUE-RES-SiO₂ formulation demonstrated significantly enhanced antioxidant capacity in DPPH, CUPRAC, and ABTS assays, surpassing free resveratrol. Strong anti-inflammatory capacity was also observed in a heat-induced protein denaturation model, with up to 75% inhibition, comparable to free quercetin and the reference drug diclofenac. In ovarian adenocarcinoma SKOV-3 cells, the formulation exhibited efficient nanoparticle uptake; however, it did not induce cytotoxicity or reactive oxygen species (ROS) production within 24 hours, likely due to slow-release kinetics, intrinsic chemoresistance of the cell line, and low concentrations tested over a limited time. Overall, these results demonstrate that MSNs constitute an effective platform for the co-delivery of quercetin and resveratrol, enhancing their stability and antioxidant and anti-inflammatory potential while overcoming key physicochemical limitations. Although anticancer effects were not observed under the tested conditions, this study establishes a robust foundation for future optimization of release kinetics, dosing strategies, and targeting mechanisms to exploit the therapeutic potential of polyphenols in oxidative stress- and inflammation-driven chronic diseases.
- Antioxidant, anti-inflammatory, and anti-adipogenic effects of micronutrient-biofortified chickpea sprouts: a potential functional ingredient for mitigating obesity-related alterations(Instituto Tecnológico y de Estudios Superiores de Monterrey, 2024-12-02) Espriu Corella, Susana María; Antunes Ricardo, Marilena; emipsanchez; Reza Zaldívar, Edwin E.; School of Engineering and Sciences; Campus Monterrey; Serrano Sandoval, Sayra NayelyObesity, a global health problem, involves excess body fat, inflammation, andoxidative stress. Despite high calorie intake, people with obesity often lack essential nutrients, a phenomenon known as "hidden hunger." Micronutrient supplementation, especially through germination biofortification, can improve micronutrient and antioxidant levels. Chickpeas are effective in this context due to their high isoflavone content, offering antioxidant, anti-inflammatory, and anti-adipogenic properties, with biofortified selenium (Se) and zinc (Zn) enhancing these health benefits. This study aimed to assess the antioxidant, anti-inflammatory, and anti-adipogenic effects of Se and Zn biofortified chickpea flour digests to explore its potential as a functional ingredient for obesity-related improvements. The characterization of the isoflavones present before and after an in vitro digestion process was evaluated, along with the quantification of the concentrations (µg of mineral/ g of flour) and bioaccessibility (%) of minerals present. Also, the evaluation of the antioxidant activity of the chickpea flour digests was assessed. Moreover, the evaluation of nitric oxide production (%) in the RAW 264.7 cell-line was employed as an anti-inflammatory activity indicator. The evaluation of lipid accumulation through Oil Red O staining in the 3T3-L1 cell line was developed to determine an anti-adipogenic effect, the determination of glycerol release was assessed as an indicator of lipolysis, along with cholesterol and triglyceride levels. Finally, mRNA gene expressions were determined through qPCR. The Germinated Control presented higher percentages of mineral bioaccessibility. All treatments presented antioxidant activities from 86% to 90% (ABTS), and 27% to 35% (DPPH). All treatments presented significantly lower productions of nitric oxide, except for ZnSO4+Na2SeO3 (15.625 µg/mL). Cells treated with ZnSO4+Na2SeO3 chickpea digests at both concentrations presented significant lower percentages of
- Investigating the bioactive properties of spearmint, orange peel, and salvia microphylla oleoresins extracted via supercritical CO2: anti-obesogenic, antioxidant, anti-Inflammatory, and neuroprotective effects and their integration into dark chocolate(Instituto Tecnológico y de Estudios Superiores de Monterrey, 2022) Chávez Delgado, Emily Lorena; Jacobo Velázquez, Daniel Alberto; mtyahinojosa, emipsanchez; Hernández Brenes, Carmen; Pérez Carrillo, Esther; Escuela de Ingeniería y Ciencias; Campus MonterreyOleoresins, with their potential health benefits, hold promise as functional ingredients, but there is scarce scientific information on their bioactivities and applications. This thesis focused on characterizing and assessing the anti-obesogenic, antioxidant, anti-inflammatory, and neuroprotective properties of spearmint (SP-O), orange peel (OP-O), and Salvia microphylla (SM-O) oleoresins extracted using supercritical CO2. These oleoresins were subsequently incorporated into dark chocolate, and their impact on physicochemical and sensory properties was evaluated. Dehydrated leaves of SP and SM, and OP were ground and subjected to supercritical CO2 extraction (2,200 psi, 8 hrs, 40°C). The extracts were characterized for carotenoid and phenolic compound composition through liquid chromatography and volatile compounds using gas chromatography. Emulsions (SP-E, SM-E, OP-E) were prepared using Tween 80 (3:1; v/v) to homogenize both fractions (oleoresin and aromatic water) present in the crude extract, and their anti-obesogenic, antioxidant, anti-inflammatory, and neuroprotective properties were evaluated in vitro with 3T3-L1, Caco-2, Raw 265.7, and SH-SY5Y cell lines, respectively. Furthermore, dark chocolate was added with these oleoresin emulsions, and their impact on water activity, color, texture, rheology, and sensory properties were assessed. The results indicated that these oleoresins are rich sources of phenolic compounds, carotenoids, and volatile compounds. SP-O was abundant in vanillin, all-trans-β-carotene, and linolenic acid. In the case of OP-O, the major compounds identified were t-cinnamic acid, all-trans-β-carotene, and D-limonene. Moreover, SM-O was rich in podophyllotoxin, all-trans-β-carotene and pulegone. In vitro studies suggested that SP-E, OP-E, and SM-E exhibited the ability to inhibit both reactive oxygen species (ROS) by 80%, 51%, 37%, respectively and nitric oxide (NO) production by 48%, 43%, 48%, respectively. Only OP-E displayed neuroprotective effects. Both SM-E and OP-E inhibited lipid accumulation by 30% and 25%, respectively. When fortified with these emulsions, the chocolate formulations exhibited softer texture, lower water activity, and a solid-like behavior. Notably, the OP-E formulation received the highest sensory preference. In summary, these oleoresins hold potential as nutraceutical agents for mitigating the development of metabolic syndrome and its associated pathologies, given their demonstrated anti-obesogenic, anti-inflammatory, antioxidant, and neuroprotective attributes. Furthermore, incorporating these emulsified oleoresins into chocolate matrices presents a viable strategy for the formulation of functional foods; nevertheless, further research is needed to elucidate both preventive and therapeutic efficacy within these fortified chocolates.