Ciencias Exactas y Ciencias de la Salud

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In silico identification of cis-regulatory elements in folate biosynthesis and 1C metabolism genes in plants
(Instituto Tecnológico y de Estudios Superiores de Monterrey, 2021-11-26) Salinas Espinosa, Jessica Pamela; TREVIÑO ALVARADO, VICTOR MANUEL; 205076; Treviño Alvarado, Víctor Manuel; puemcuervo; Cuevas Díaz Durán, Raquel; Rodríguez López, Carlos; Martínez Ledesma, Juan Emmanuel; School of Engineering and Sciences; Campus Monterrey; Díaz de la Garza, Rocío Isabel
Folates (vitamin B9) are enzyme cofactors required for all organisms for one-carbon (1C) transfer reactions. A deficiency of these nutrients can lead to several health problems. Since humans are not natural producers of folates, the intake of these nutrients from plants is vital for human nutrition. Several techniques that involve the genetic modification of organisms have proved to be effective for the fortify plants with essential macronutrients. However, to achieve this, it is necessary to elucidate the metabolic control in plant systems. Although the genes involved in folate biosynthesis and 1C metabolism in plants are known, the mechanisms of transcriptional regulation have not yet been explored. This project focuses on discovering cis-regulatory DNA elements (motifs) using computational data analysis to provide insights regarding the regulation of folate biosynthesis in plants. For this, we first collected a compendium of known genes related to folate biosynthesis. Then, a database comprising the DNA promoter regions of folate biosynthesis and 1C metabolism genes in 19 different plant species was built and analyzed using different motif discovery algorithms. Afterward, the discovered motifs were tested for statistical significance and further associated with their putative biological role using other bioinformatics tools. A total of 149 statistically significant motifs (p < .05) were discovered in 18 of 19 species using the GimmeMotifs ensemble algorithm. These motifs were represented in 104 different regulatory networks built automatically from co-expression clusters obtained from each plant species. The results from this work could provide an insight into the transcriptional regulation of the folate biosynthesis pathway in plants. Furthermore, the elements found could be used for research in gene editing techniques to produce biofortified crops.
Metabolic flux analysis of Xanthophyllomyces dendrorhous metabolism to understand the production of astaxanthin
(Instituto Tecnológico y de Estudios Superiores de Monterrey, 2020-06) Martínez Castro, Victor Ignacio; MATA GOMEZ, MARCO ARNULFO; 207149; Mata Gómez, Marco Arnulfo; lagdtorre, emipsanchez; Benavides Lozano, Jorge Alejandro; Licona Cassani, Cuauhtémoc; School of Engineering and Sciences; Campus Toluca; Goméz Sánchez, Carlos Eduardo
Carotenoid production by microorganisms, contrary to chemical synthesis, could fulfill the in-creasing demand for human consumption. The yeastX. drendrorhousis one of the most promis-ing and economically attractive microorganisms for industrial production of astaxanthin. Themetabolic pathway through which this yeast synthesize this valuable carotenoid is known. How-ever, the complex mechanisms that are involved in the process, the distribution of the metabolicfluxes and the rates at which the pathways work, remain unknown. Several studies have provideddifferent approaches to manipulate and improve carotenoid production inX. dendrorhousfromclassical mutagenesis to genetic engineering of the complete pathway covering improved precur-sor supply for carotenogenesis, enhanced metabolite flow into the pathway, and manipulating therelation C/N in the culture medium. However, it has not been reported quantitatively how nutri-ents, from the central metabolism and other pathways, converge in the carotenoids biosynthesis.In this study, the metabolism ofX. dendrorhousgrowing in a continuous culture, under two am-monia conditions, Limited (L) and Non-limited (NL). The metabolic flux analysis (MFA) allowedto understand the distribution the intracellular fluxes along the different metabolic pathways eval-uated, but most important, it elucidated that by limiting the ammonia assimilation flux(L= 0.002±1.1 E-05; NL= 0.004±4.3 E-05; g/gcellh), a promotion of the astaxanthin formation flux wasobtained (L= 86.4±0.6; NL= 0; ug/gcellh). This first approach will help to set a deeper studyin order to understand the metabolic pathways that regulate the flux towards the astanxanthinbiosynthesis

Ciencias Exactas y Ciencias de la Salud

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