Ciencias Exactas y Ciencias de la Salud
Permanent URI for this collectionhttps://hdl.handle.net/11285/551039
Pertenecen a esta colección Tesis y Trabajos de grado de las Maestrías correspondientes a las Escuelas de Ingeniería y Ciencias así como a Medicina y Ciencias de la Salud.
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- Development of a GelMA-based bioink enhanced with minimally-processed tissue for the fabrication of skeletal muscle(Instituto Tecnológico y de Estudios Superiores de Monterrey, 2020-06-12) Tavares Negrete, Jorge Alfonso; TAVARES NEGRETE, JORGE ALFONSO; 744164; Trujillo de Santiago, Grissel; ilquio/puemscuervo; Pérez Carrillo, Esther; Mertgen, Anne-Sophie; Santiago Miramontes, Ma. de los Ángeles de; Escuela de Ingeniería y Ciencias; Campus Monterrey; Álvarez, Mario MoisésBioprinting, an emerging technology that uses living cells and biomaterials for fabricating engineered tissues with complex three-dimensional (3D) architecture, has become popular as an alternative to conventional 3D-culture techniques. In typical extrusion bioprinting, a bioink (i.e., generally a suspension of cells in a hydrogel) is extruded through a printing head to build an artificial 3D tissue in a layer-by-layer-fashion. Bioinks are key components of a bioprinting process. Bioinks have to meet appropriate biological and rheological characteristics to be printable and to provide a proper microenvironment to cells. Here, we present a simple strategy to develop cost-effective bioinks based on gelatin methacryloyl (GelMA) enhanced with fetal minimally processed muscle tissue (MPT). This strategy is intended to supplement bioinks with growth factors, glycosaminoglycans and proteins from fetal tissue, due to its biochemical composition rich in growth factors and peptides. As a first demonstrative model, we supplemented GelMA hydrogels with 0.5, 1, 2% dried and powdered MPT derived from a goat or calf fetus. The biochemical characterization of MPT showed that our minimally processed technique preserves more than 65% of GAG content compared to traditional methods (i.e. decellularization). The rheological profile of our hydrogels was analyzed to determine a suitable working-window of printing parameters, all inks have shear thinning behavior. Cell-culture experiments showed that the incorporation of MPT in the hydrogels influences the metabolic activity of the myoblast cells (C2C12) and confers structural stability to the hydrogels for cultures of up to 28 days in comparison to pristine GelMA hydrogels. The cell orientation of the GelMA-MPT bioprinted constructs (measured with image analysis) was up to 60% resembling the tissue coherence and architecture of native tissue. Our results demonstrate that our materials can be used as cost-effective bioinks for the bioprinting of skeletal muscle models.
- Volumetric bioprinting for medical applications(Instituto Tecnológico y de Estudios Superiores de Monterrey, 2020-06-05) López Franco, Arturo; VARGAS ROSALES, CESAR; 33901; Vargas Rosales, César; RR; Zhang, Yu Shrike; School of Engineering and Sciences; Campus Monterrey; Galaviz Aguilar, José AlejandroAdditive manufacturing (3D printing) has been a widely used tool in a lot of different industries. Among these industries can be found tissue engineering and regenerative medicine, since bioprinting is one of the main techniques applied. The implementations of new technologies for additive manufacturing, have been adapted into the bioprinting area for medical purposes. Additive manufacturing technologies have been evolving from printing point-to-point, layer-by-layer, and more recently volumetric printing, which represents printing a whole volume simultaneously. In this thesis is presented a new technique for bioprinting, the Computed Axial Lithography (CAL) printing, which is a recently additive manufacturing technology based on reconstruct- ing a volume simultaneously, has demonstrated to have advantages against other additive manufacturing techniques, improving the printing speed, the resolution, minimum material waste, and more, and its application in the medicine industry has not been explorer looking very promising for this research field with limitless applications. The bioink used in the experiments presented is a GelMA-based hydrogel, and the 3D structures achieved should be capable of present biocompatibility with living organisms. For this thesis, the reproduction of the CAL printing for biomaterials, in this particular case GelMA, is proved, opening the doors for applying the same concept to different biomaterials, which could have limitless applications in many distinct research areas.
- Biofabrication of nanoenhanced hydrogel fibers for muscle tissue engineering using surface chaotic flows: Chaotic 2D-printing(Instituto Tecnológico y de Estudios Superiores de Monterrey, 2020) Frías Sánchez, Ada Itzel; TRUJILLO DE SANTIAGO, GRISSEL; 256730; FRIAS SANCHEZ, ADA ITZEL; 887018; ALVAREZ, MARIO MOISES; 26048; Trujillo de Santiago, Grissel; RR; Tamayol, Ali; Ponz Ascaso, Fernando; Samandari, Mohamadmahdi; School of Engineering and Sciences; Campus Monterrey; Alvarez, Mario MoisésMultiple human tissues exhibit a fibrous nature. Therefore, the fabrication of hydrogel filaments for biomedical engineering applications is a trending topic. Current tissue models are made of materials that often require further enhancement for appropriate cell attachment, proliferation and differentiation. Here we present a simple strategy, based on the use of mathematically modelable surface chaotic flows, to fabricate continuous, long and thin filaments of gelatin methacryloyl (GelMA) added with Turnip mosaic virus (TuMV) for enhanced muscle tissue engineering. The fabrication of these filaments was achieved by chaotic advection in a finely controlled and miniaturized version of the journal bearing (JB) system. A drop of a pre-gel solution of GelMA was injected on a higher-density viscous fluid (glycerin) and a chaotic flow was applied through an iterative process. The hydrogel drop exponentially deformed and elongated to generate a fiber, which was then photocrosslinked under exposure to UV light. Computational fluid dynamics (CFD) simulations were conducted for the design and prediction of our results. GelMA fibers were then used as scaffolds for C2C12 myoblast cells, and the effect of adding plant-based viral nanoparticles (VNP) to the hydrogel fibers as nano-scaffolds for cellular growth was evaluated. Chaotic 2D-printing was proven to be a viable method for the fabrication of hydrogel fibers. CFD simulations accurately predicted the lengths of the printed fibers, and a correlation coefficient of R2=0.9289 was determined from the experimental and simulated data of the first two cycles. The hydrogel fibers were effective scaffolds for muscle cells and show potential to be used as cost-effective models for muscle tissue engineering purposes. TuMV significantly increased the metabolic activity of the cell-seeded fibers (p<0.05), strengthened cell attachment throughout the first 28 days, improved cell alignment to ~50%, and promoted the generation of structures that resemble natural mammal muscle tissue.