Ciencias Exactas y Ciencias de la Salud
Permanent URI for this collectionhttps://hdl.handle.net/11285/551039
Pertenecen a esta colección Tesis y Trabajos de grado de las Maestrías correspondientes a las Escuelas de Ingeniería y Ciencias así como a Medicina y Ciencias de la Salud.
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- Design and characterization of an adhesive and pH-indicating hydrogel with gentamicin release as a proof of concept for its potential use as an acute wound dressing(Instituto Tecnológico y de Estudios Superiores de Monterrey, 2021-12-06) Viaña Mendieta, Pamela; Benavides Lozano, Jorge Alejandro; puemcuervo; Antunes Ricardo, Marilena; Mata Gómez, Marco Arnulfo; School of Engineering and Sciences; Campus Monterrey; Sánchez, Mirna LorenaWound care cost is an overwhelming problem aggravated by the burning of skin injuries and the overuse of traditional materials that are low cost-efficient. Modern wound care strives for multifunctional wound dressings to monitor physiological conditions and prevent wound infection or non-healing processes. Thus, this study addresses the synthesis and characterization of an adhesive and pH-indicating hydrogel dressing with gentamicin release for the potential acceleration and monitoring of acute wounds. The proposed hydrogels were prepared by physical and chemical crosslinking of polyvinyl alcohol (PVA), phenol red, glycerol, citric acid (CA), butyl-cyanoacrylate (BCA), and gentamicin. Diverse formulations, varying PVA and CA concentration, were evaluated and selected by the stable swelling profile. Then, the selected formulation was characterized by swelling degree, colorimetric pH-change evaluations, adhesion and dehydration assay, and drug release profile. The selected formulation was 4% wt. PVA, 5% wt. CA, 10% wt. glycerol and 1.5% wt. BCA, and showed excellent properties as a wound dressing. It had uniform transparency, a good pH-indicating property, enough water content, stable swelling degree and gentamicin release. Thus, the pH-indicating property showed the transition of pH 4 to 9, with sensitive pH change by forming the circle-like pattern at pH 8 and 9. The dehydration ratio was 1.0 and maintained adhesion to a plastic surface for 96 h. The release of gentamicin was enough in 24 h (96.3 %). Based on the results, this proof of concept concludes that the designed multifunctional hydrogel has suitable properties as a potential wound dressing. This study shows the opportunity to continue its characterization in detail in searching for a potential commercial wound dressing.
- Use of nanoparticles of copper with plant extracts as an alternative against multidrug-resistant bacteria causing urinary tract infections(Instituto Tecnológico y de Estudios Superiores de Monterrey, 2021-08-29) De la Rosa Santana, Uriel; TORRES HUERTA, ANA LAURA; 207433; Torres Huerta, Ana Laura; emipsanchez; Hernández Cooper, Ernesto Manuel; Ordaz Cortés, Alberto; Tapia Ramírez, José Isabel; Escuela de Ingeniería y Ciencias; Campus Estado de México; António Pérez, AuroraThere is increasing concern due to the high incidence of multi-resistant bacteria. The importance of these microorganisms is in the economic and health impact that cause at global level in different countries. Many efforts have been made to improve drugs to attack these bacteria, however in the long term this only aggravates the situation. Due to this, alternatives that allow to reverse this situation are necessary. Thanks to the development of nanotechnology, the importance of nanoparticles has increased in the last years to fight infections. The green synthesis process is particularly appealing. It has been reported the use of plant extracts as reducing agents allows obtaining metallic nanoparticles with antibacterial activity. That is why in this project copper nanoparticles (CuNPs) with green synthesis were obtained. For this, extracts of Azadiractha Indica (Neem) and Eysenhardtia Polystachia (Palo Azul) were used given a previously reported medicinal properties in urinary tract infections and their high content of metabolites as reducing agents for the formation of nanoparticles (NPs). The extracts were characterized at different conditions of time and temperature, by IFTR (Fourier transform infrared) and UV-vis. Following this, mixtures of the selected extract with solutions of copper precursor salt at a specific concentration were heated under constant stirring for 20 h, taking samples at 4-hour intervals. The obtained nanoparticles were analyzed in terms of distribution, size, morphology, and concentration through dynamic light scattering (DLS) and scanning electron microscopy (SEM). Meanwhile, the characterization of its antimicrobial effect was determined thanks to absorbance analysis in liquid culture and plate reader. It was observed that as the synthesis time passes, the size of the nanoparticle tends to reduce. On the other hand, its morphology shows signs of being colloidal, although not uniformly. Meanwhile, when comparing the nanoparticles made with both extracts and different precursor salts, a different size trend was observed in nanoparticles. in terms of antibacterial activity, it was observed that the synthesis time at 20 h of the process shows an insight of bacteriostatic activity compared to a shorter stirring time. Meanwhile an indication of concentration-dependent inhibition was observed. On the other hand, it was observed that the combination of Neem extract with copper nitrate precursor salt is the best option for inhibition of bacteria. However, it is necessary to carry out more studies of antibacterial effects to guarantee its effectiveness to be an alternative to conventional antibiotic treatments.
- Production and characterization of a multilayer thin film by pvd with antibacterial activity for biomedical applications(Instituto Tecnológico y de Estudios Superiores de Monterrey, 2021) Pérez Ávila, Juana Yessica; Oseguera Peña, Joaquín Esteban; 9202; Oseguera Peña, Joaquín Esteban; emijzarate/puemcuervo; Melo Máximo, Lizbeth; School of Engineering and Sciences; Campus Estado de México; Melo Máximo, Dulce ViridianaThis thesis for the Production and Characterization of a Multilayer Thin Film by PVD with Antibacterial Activity for Biomedical Applications is for the Master’s Program in Nanotechnology at ITESM CEM. Millions of people use some form of implant in their bodies and infection is the most serious complication affecting to an important percentage of patients, causing postoperative prosthesis failure, chronic pain and immobility. This thesis aims to develop and characterize a multilayer thin film of CrN, ZnO and PEG on the substrate Ti6Al4V to be used in implants obtaining antibacterial activity and an improvement in the mechanical and morphological properties. For the deposition process, Reactive Magnetron Sputtering was used, and the characterization was performed using Optical Microscope, SEM, EDS, XRD, AFM, Nano-Indentation, Rockwell C test using VDI 3198 norm, Contact Angle, Tribometer and Colony Forming Units Counting. The results showed an improvement in the tribological characteristics and positive antibacterial action.
