Ciencias Exactas y Ciencias de la Salud
Permanent URI for this collectionhttps://hdl.handle.net/11285/551039
Pertenecen a esta colección Tesis y Trabajos de grado de las Maestrías correspondientes a las Escuelas de Ingeniería y Ciencias así como a Medicina y Ciencias de la Salud.
Browse
Search Results
- Synthesis, optimization, physicochemical characterization, and in vitro evaluation of Solid Lipid Nanoparticles for the improved delivery of Polyphenols across a phospholipid bilayer model(Instituto Tecnológico y de Estudios Superiores de Monterrey, 2024-12-11) Alfaro Almaguer, Juan Antonio; Chávez Santoscoy, Rocío Alejandra; emimmayorquin; Cabrera Ramírez, Angel Humberto; Vázquez Lepe, Elisa Virginia; Campus Monterrey; Sierra Valdés, FranciscoPolyphenols are one of the most common phytochemicals, with a high relevance on human diet due to its wide presence on edible plants. Particularly, quercetin and curcumin stands out for its beneficial bioactivities, mostly related to their antioxidant and anti-inflammatory effects. However, its therapeutical potential is not fully exploited due to their low bioavailability and poor water solubility, being rapidly excreted from the body or metabolized. To overcome those drawbacks, the objective of this research project is to evaluate and optimize the incorporation of those compounds into nanoparticle formulations. For those purposes, Solid Lipid Nanoparticles (SLN) made of Cetyl palmitate and Tween® 80 were synthetized. The optimization process allowed synthetize quercetin- or curcumin-loaded SLN with diameter and size below 200 nm and 0.2, respectively, which in first instance allows the system to be a suitable candidate for delivery applications. SLN zeta-potential of loaded SLN was also measured, with -3.5756 ± 0.6577 and -4.1089 ± 0.5921 mV for quercetin- and curcumin-loaded SLN, respectively. Next, a Multilamellar Vesicle (MLV) model from 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) and cholesterol were synthetized to mimic the properties of the cellular lipid membrane and to calculate the delivery efficiency of quercetin and curcumin-loaded SLN, giving a respective 16 and 81%. Finally, the safety of the SLN system was addressed by means of cytotoxicity assays on HDFA cells, which showed no inhibition of cell viability among all the unloaded SLN treatments, with a maximum of 14.51 mg/mL. Quercetin- and curcumin-loaded SLN showed cytotoxicity at 150 and 245 μM, respectively. The system showed proper physicochemical characteristics suitable for delivery applications, and its proved safety allows to perform further research focused on a specific target tissues and diseases.
- Synthesis and biotriborheological characterization of shea butter solid lipid nanoparticles in topical formulation(Instituto Tecnológico y de Estudios Superiores de Monterrey, 2020-11-23) Aviles Castrillo, Jose Ivan; MEDINA MEDINA, DORA ILIANA; 40536; Medina Medina, Dora Iliana; tolmquevedo; Valencia Lazcano, Anai Alicia; School of Engineeting and Science; Campus Estado de México; Quintanar Guerrero, DavidSolid Lipid Nanoparticles (SLN) are pharmaceutical delivery system and pharmaceutical formulation that provide great biocompatibility and efficiency of encapsulation. The current challenge is the storage of these due to the time they agglomerate reducing their properties. An effective way to validate its stability in storage is conducting rheology studies. Shea Butter SLN is used, with the purpose of having a higher antioxidant effect. It has been performed the synthesis, by hot homogenization technique, with an excellent stability. We validated its penetration capacity and perform different characterizations as for example Atomic Force Microscopy (AFM), Confocal Microscopy, Scanning Electron Microscopy (SEM), DSC, DLS and Zeta potential. It gave us an mean particle size of 213 nm, with a zeta potential of -40 mV and obtaining a circular morphology; Therefore, we can affirm that a correct synthesis of these nanoparticles was carried out, due to their size and stability since after 3 months of storage they did not show significant growth. Because of Confocal Microscopy studies, due to their property that SLNs are autoflowering, it was possible to validate that Shea Butter Solid Lipid Nanoparticles can penetrate the epidermis. Triborheological studies were carried out such as Oscillatory stress sweep, Viscosity/shear rate profile, Normal stress profile and Sliding speed sweep in this way we identify and quantify the impact of Solid Lipid Nanoparticles in topical formulations. It was discovered that SLNs had lower Coefficient of Friction than those containing bulk lipids. SLNs in topical formulations have potential applications in the cosmetic as anti-aging agents this due to its antioxidant properties, skin occlusion, increased skin hydration and potential antiinflammatory properties.