Ciencias Exactas y Ciencias de la Salud
Permanent URI for this collectionhttps://hdl.handle.net/11285/551039
Pertenecen a esta colección Tesis y Trabajos de grado de las Maestrías correspondientes a las Escuelas de Ingeniería y Ciencias así como a Medicina y Ciencias de la Salud.
Browse
Search Results
- Engineered mesoporous silica nanoparticles for the co-delivery of quercetin and resveratrol: structural characterization and assessment of antioxidant and anti-inflammatory potential(Instituto Tecnológico y de Estudios Superiores de Monterrey, 2025-12-02) Torres Copado, Andrea; Paul, Sujay; mtyahinojosa, emipsanchez; Arvizu Espinosa, María Goretti; Sahare, Padmavati; School of Engineering and Sciences; Campus Monterrey; Estévez González, Miriam RocíoThe global burden of noncommunicable diseases (NCDs) is closely associated with persistent oxidative stress and chronic inflammation. Natural polyphenols such as quercetin and resveratrol possess potent antioxidant and anti-inflammatory activities; however, their therapeutic potential is severely hindered by low aqueous solubility, poor chemical stability, and rapid metabolic degradation. Nanotechnology-based delivery systems offer a promising approach to enhance the bioavailability and functional performance of these bioactive compounds. Accordingly, this work aimed to co-encapsulate quercetin and resveratrol into mesoporous silica nanoparticles (MSNs), thoroughly characterize the resulting nanocarrier system, and assess its biological properties in vitro. MSNs were synthesized through a modified Stöber method, yielding uniform, spherical, amorphous nanoparticles with an average hydrodynamic diameter of ~126 nm, a high specific surface area (200.3 m²/g), a pore volume of 0.445 cm³/g, and a mean pore diameter of 5.4 nm. Co-loading was achieved using a solvent evaporation method, resulting in high encapsulation efficiencies (79.9% for quercetin and 71.4% for resveratrol). Physicochemical characterization (FTIR, XRD, TGA, DLS, Zeta Potential) confirmed successful drug incorporation, partial amorphization of the polyphenols, enhanced thermal stability, and a sustained release profile extending to 75 hours. The QUE-RES-SiO₂ formulation demonstrated significantly enhanced antioxidant capacity in DPPH, CUPRAC, and ABTS assays, surpassing free resveratrol. Strong anti-inflammatory capacity was also observed in a heat-induced protein denaturation model, with up to 75% inhibition, comparable to free quercetin and the reference drug diclofenac. In ovarian adenocarcinoma SKOV-3 cells, the formulation exhibited efficient nanoparticle uptake; however, it did not induce cytotoxicity or reactive oxygen species (ROS) production within 24 hours, likely due to slow-release kinetics, intrinsic chemoresistance of the cell line, and low concentrations tested over a limited time. Overall, these results demonstrate that MSNs constitute an effective platform for the co-delivery of quercetin and resveratrol, enhancing their stability and antioxidant and anti-inflammatory potential while overcoming key physicochemical limitations. Although anticancer effects were not observed under the tested conditions, this study establishes a robust foundation for future optimization of release kinetics, dosing strategies, and targeting mechanisms to exploit the therapeutic potential of polyphenols in oxidative stress- and inflammation-driven chronic diseases.
- Evaluation of the Synergistic Effects of Curcumin-Resveratrol co-loaded Mesoporous Silica Nanoparticles on Colorectal Cancer Cells(Instituto Tecnológico y de Estudios Superiores de Monterrey, 2023-12-04) Ochoa Sánchez, Adriana; Paul, Sujay; emimmayorquin; Arvizu Aguilar, María Goretti; EIC Escuela de Ingeniería y Ciencias; Campus Monterrey; Estévez González, Miriam RocíoColorectal cancer (CRC) poses a substantial global health challenge, necessitating innovative solutions due to the limitations inherent in current conventional treatments. Curcumin and resveratrol, natural phytochemicals, have emerged as promising anticancer agents, offering therapeutic benefits with minimal toxicity to healthy tissues. However, the clinical application of these phytochemicals faces limitations. Nanoformulations have gained prominence for their ability to overcome these constraints, ensuring improved solubility, controlled release, and enhanced biocompatibility. Mesoporous silica nanoparticles (MSNs) have garnered attention as nanocarriers due to their exceptional biocompatibility and high load capacity. This study focused on investigating the effect of co-loaded curcumin and resveratrol MSNs on target gene expression, which is crucial in cancerogenesis. Curcumin and resveratrol were immobilized onto MSNs functionalized with 3- aminopropyltriethoxysilane (APTES) and glutaraldehyde (GTA). The co-loaded MSNs were characterized using FTIR, TGA, SEM, XRD, BET, and determination of encapsulation efficiency by UV-Visible spectrophotometry. In vitro cytotoxicity assays were conducted using HCT-116 and Caco-2 colorectal cell lines. Target gene expression was evaluated through RT-qPCR after treating HCT-116 cancer cells with the nanoformulation at 24 and 48-hour time points. The results revealed significant upregulation of the tumor suppressor gene TP53 and apoptosis-related gene Bax, coupled with downregulation of proliferation-related genes Wnt-1 and CTNNB1. These findings demonstrate the ability of co-loaded curcumin and resveratrol MSNs to modulate key genes involved in cancer progression, showcasing their potential as a promising therapeutic strategy for CRC.