Ciencias Exactas y Ciencias de la Salud
Permanent URI for this collectionhttps://hdl.handle.net/11285/551039
Pertenecen a esta colección Tesis y Trabajos de grado de las Maestrías correspondientes a las Escuelas de Ingeniería y Ciencias así como a Medicina y Ciencias de la Salud.
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- Digital light processing additive manufacturing for accessible blood-brain barrier organ-on-a-chip fabrication(Instituto Tecnológico y de Estudios Superiores de Monterrey, 2025-06) Lagunes Nava, Daniel; García Farrera, Brenda; emimmayorquin; Magaña Aguirre, Jonathan Javier; García Aguirre, Ian Alain; Cano Quiroz, Anaid; School of Engineering and Sciences; Campus Estado de México; Solis Cordova, José de JesúsOrgan-on-a-Chip (OoC) technologies represent a promising alternative to traditional preclinical models and their actual limitations, yet their widespread adoption remains limited by cost, fabrication complexity, and accessibility. This thesis presents the development of an economically viable microfluidic platform designed to mimic the Blood-Brain Barrier (BBB) using Digital Light Processing (DLP) additive manufacturing. By leveraging the geometric freedom and rapid prototyping capabilities of DLP, a series of chips were fabricated and systematically evaluated through both structural characterization and functional assays. The platform’s performance was assessed via passive diffusion experiments using sodium chloride, providing a quantifiable readout of molecular transport across the chip interface. Particular emphasis was placed on the role of channel geometry in shaping diffusion behavior. Comparative analysis of square and circular layouts demonstrated that structural configuration alone can influence transport dynamics, even under equivalent flow conditions, an observation reinforced by simplified computational simulations. These findings call into question the extent to which current chip designs, often simplified because of the nature of the techniques, truly replicate physiologically relevant transport. Results revealed that the square chip exhibited faster and more direct fluid penetration through the interface, while the circular design induced more distributed flow with attenuated velocity vectors. This divergence also reflected in the diffusion curves, challenges the conventional assumption that greater surface area alone enhances transport, and emphasizes the need to reevaluate geometric decisions in microfluidic design. Beyond functionality, the fabrication process itself validated the feasibility of low-cost and reproducible production of complex microfluidic architectures. Together, these findings reaffirm the potential of DLP printed devices as accessible tools for biomedical research and establish a foundation for more physiologically relevant Organ-on-a-Chip systems.
- Development of a multi-component disjointed tissue culture system using three-dimensionally printed polymeric scaffolds and microfluidic pumping(Instituto Tecnológico y de Estudios Superiores de Monterrey, 2024-12) Romero Zepeda, Claudia Alejandra; Lozano Sánchez, Luis Marcelo; emipsanchez; Perfecto Avalos, Yacanxóchitl; García González, Alejandro; García Varela, Rebeca; School of Engineering and Sciences; Campus León; Chaires Oria, Jorge IsaacIn-vitro cellular culture plays a crucial role in preclinical research. While cost-effective, the pre- vailing 2D culture approach falls short in simulating realistic cellular interactions when these cells are grown in different but interacting spaces. Organs-on-a-Chip (OoC) devices have been developed to address this limitation, creating controlled micro-environments that mimic in-vivo tissue interaction conditions. This research addressed designing and assessing a microfluidic chip device based on ad- ditive manufacturing to analyze fibroblast and monocyte cell interaction grown in a separate culture apparatus. The OoC devices were created using Computer-Aided Design (CAD), and additive manu- facturing strategies using translucent resin as constructive material. The developed chip consisted of 200 mm2 cell culture area, a glass window for monitoring, and two inlets and outlets for fluid transfer and sampling. An instrumented peristaltic micro-pumping system induces fluid motion through the tubing that connects the manufactured microchips. Here, we show the ability of the developed 3D printed system to culture different cell lines, allow treatment addition without disturbing the system, and connect with a continuous flow between the devices without generating detectable cellular stress by enzymatic quantification. Finally, the interconnected system communicates between fibroblast and monocyte cultures by connecting two chips with micropumps through microscopic and cellular stress markers in selected cell lines. This results in a prototype for a multi-organ-on-a-chip-like device.