Ciencias Exactas y Ciencias de la Salud
Permanent URI for this collectionhttps://hdl.handle.net/11285/551039
Pertenecen a esta colección Tesis y Trabajos de grado de las Maestrías correspondientes a las Escuelas de Ingeniería y Ciencias así como a Medicina y Ciencias de la Salud.
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- Fabrication and characterization of an anion exchange-based monolithic column, using γ-aminobutyric acid as a ligand, contained in a 3D printed casing for separation of biomolecules(Instituto Tecnológico y de Estudios Superiores de Monterrey, 2021-09-22) Ceballos Medina, Javier; Mata Gómez, Marco Arnulfo; dnbsrp; Ornelas Soto, Nancy Edith; Gordillo Guerra, Paola Guadalupe; Escuela de Ingeniería y Ciencias; Campus Monterrey; Ibarra Herrera, Celeste ConcepciónA poly(GMA-co-EDMA) monolith was synthesized within a 3D printed housing of 4 different materials and modified with γ-aminobutyric acid, a non-protein amino acid. The monoliths were synthesized with 3 different surface pretreatments in housing with 5 mm i.d. and 20 mm length channel. The functionalization of the monolith was done by by Shiff-based method using diethylenediamine as a spacer. SEM images evidenced that a homogeneous surface monolith on γ-MAPS treated surfaces while direct synthesis had scales and big agglomerates within the polymeric matrix. Raman spectroscopy evidenced the different modifications the resin housing surfaces experienced and confirmed the presence of the ligand. On the other hand, BET analysis found surface areas ranging 7.459 m2/g to 20.147 m2/g and micropores range from 0.432 to 1.736 nm, being 4.70 nm the most common pore. The fabricated monolithic columns with EDMA-G, γ-MAPS at 20%, γ-MAPS at 30% treatments exhibited ionic capacities of 0.056±0.001, 0.174±0.001 and 0.293±0.015 mmol Cl⁻/mL, respectively. EDMA-G column exhibited the best performance for fractionating proteins from a concentrated bacterial lysate from E. coli strain BL21 star with plasmid pSB21C3, producing RFP. Eventhough the column was able to retain 20% of the total RFP, improvement needs to be done to increase the ligand density and, in consequence, the adsorption capacity.
- Fabrication of a guanidine ligand-based anion exchange monolithic stationary phase in a 3D printed polypropylene housing for protein chromatography(Instituto Tecnológico y de Estudios Superiores de Monterrey, 2021-01-01) Pérez Rodríguez, Elizabeth; MATA GOMEZ, MARCO ARNULFO; 207149; Mata Gómez, Marco Arnulfo; puelquio/mscuervo; Luna Vital, Diego Armando; Sánchez, Mirna Lorena; González Valdez, José Guillermo; School of Engineering and Sciences; Campus Monterrey; Ibarra Herrera, Celeste ConcepciónA monolithic anion exchange support is synthesized based on the incorporation of γ -guanidinobutyric acid an alkaloid with three resonant amino groups- as ligand onto a poly(EDMA-co-GMA) monolith. The created monolithic anion exchange support is referred as M-Gnd. Monolith was synthesized in a one-step polymerization reaction in a designed and printed polypropylene housing within a 5x20mm i.d. channel. Functionalization of the poly-methacrylate monolith with γ -guanidinobutyric acid was done by Shift-based method using diethylenediamine as a spacer. Homogeneous surface morphology was appreciated by SEM image, and FTIR analysis confirmed the presence of ligand. Highest value of Dynamic binding capacity (12.7mg BSA/mL monolith) was obtained at 0.5 mL/min when a 2 mg/mL protein concentration was used. The estimated ligand for M-Gnd was 1.23 mmol/g dry support. The M-Gnd support allowed to purity a red fluorescent protein up to 75%, presenting a high efficiency (N and HETP values). The characterized M-Gnd showed positive results as anion exchange chromatography support for protein purification. However, the functionalization of γ-guanidinobutyric acid can be improved to have a comparable chromatographic performance to commercial anion exchange monolith.