Ciencias Exactas y Ciencias de la Salud
Permanent URI for this collectionhttps://hdl.handle.net/11285/551039
Pertenecen a esta colección Tesis y Trabajos de grado de las Maestrías correspondientes a las Escuelas de Ingeniería y Ciencias así como a Medicina y Ciencias de la Salud.
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- Design and development of a reconfigurable die for thermoforming process(Instituto Tecnológico y de Estudios Superiores de Monterrey, 2018-05-17) Reyna Yáñez, Felipe Osvaldo; Cortés Ramírez, Jorge Armando; Cárdenas Alemán, Eduardo; Aguayo Téllez, HumbertoAccording to the World Economic Forum (WEF), the nascent technologies such as Internet of Things (IoT), 3D printing, and advanced robotics represent between 70%-80% of the product market, manufacturing and services in North America and Europe. In this context, the designing and implementation of reconfigurable tools can generate more flexible processes, oriented to improve the productivity in manufacturing industry, especially in molding and thermoforming packaging. Consequently, a reconfigurable die based on shape-memory alloy actuators, is a solution for attending the industry challenges, due to its inclusion of a microstructural-based, temperature-based, and time-based constitutive model theory for its implementation. Within these challenges exists the need of fabrication of millions of pieces due to its limitation with a low-volume production, and high investment and maintenance tool costs. The objective of this thesis work is the development of a technology that allows manufacturing variable-shape packages through a reconfigurable die with a thermoforming process. The functionality and performance of the technology lie within the application of a shape-memory alloy theory for a reconfigurable system; the development of a functional prototype with both NiTi-based and stepper-based motion mechanisms; and the development of a technology roadmap that allows having a vision of the potential customer segment of the product and/or technology, according to their needs and opportunities.
- Fabricación de prótesis de mandíbula con polietileno de alta densidad por conformado incremental mono-punto(Instituto Tecnológico y de Estudios Superiores de Monterrey, 2017-12-06) Lozano Medina, José Angel; S/n; S/n; S/n; Oscar Martínez Romero; Alex Elías Zuñiga; José Manuel Diabb Zavala; Luis Manuel Palacios Pineda; División de Ingenieria y Arquitectura Programa de Graduados en Ingeniería; Campus MonterreyEl proceso de conformado incremental mono-punto (SPIF, por sus siglas en inglés) es usado para la manufactura de productos personalizados, prototipos funcionales y su aplicación en la fabricación de prótesis metálicas ha sido probada. Sin embargo, el uso de chapa/placa hechas de material termoplástico (como el Polietileno de Alta Densidad) para la manufactura de prótesis por conformado incremental mono-punto es un proceso actualmente bajo investigación y prueba. En este marco de referencia, el objetivo del presente escrito es presentar el diseño y manufactura de prótesis de mandíbula hecha con placa de Polietileno de Alta Densidad (HDPE, por sus siglas en inglés) fabricadas por el proceso de Conformado Incremental Mono-Punto (SPIF, por sus siglas en inglés) para explicar y justificar su aplicación en la reconstrucción de mandíbula. La geometría real de la mandíbula es adquirida por tomografía computarizada (CT, por sus siglas en inglés) y manipulada con el objetivo de conseguir el archivo en modelo CAD (DICOM a modelo CAD). De ahí, es que puede generar un planear un tratamiento de reconstrucción de mandíbula y se proponga una aproximación al prototipo de la prótesis antes de la cirugía del paciente.
- Characterizing the role of mitochondrial Ca2+ overload and permeability transition during the BCR-dependent activation of murine B lymphocytes(2017-12-05) Torres Quintanilla, Alejandro Daniel; González Castillo, Elena Cristina; García Rivas, Gerardo; Maravillas Montero, José Luis; Torre Amione, GuillermoMitochondria have proved their role as major players for immunometabolism, regulating cell function by metabolic signals. Indeed, mitochondrial function during lymphocyte activation goes beyond ATP supply, as recent evidence shows that mitochondrial Ca2+ uptake and mitochondrial ROS (mROS) production regulate lymphocyte activation. Furthermore, the adaptive immune response depends on the differentiation of lymphocytes into various subsets. Whether metabolic signals determine lymphocyte differentiation has just began to be explored. Interestingly, recent evidence shows that mitochondrial signals determine B lymphocyte differentiation into memory cells. During lymphocyte activation, Ca2+ uptake into mitochondria has proven to be an important mechanism of regulation. However, if mitochondrial Ca2+ uptake capacity impacts B cell differentiation and activation is not yet determined. Here, we have generated an in vitro model of B lymphocyte mitochondrial Ca2+ overload to explore whether this mechanism is involved in B cell activation. Primary mice B lymphocytes were subjected to activation or thapsigargin treatment to induce Ca2+ overload. The effect on the expression of activation surface marker CD69 was explored using flow cytometry. Mitochondrial contribution was explored by Ca2+ uptake inhibition using Ru360 or treatment with the mitochondrial antioxidant MitoTEMPO. Mitochondrial membrane potential, Ca2+ uptake, and content were determined. Results show that our in vitro model effectively induces mitochondrial Ca2+ overload, as seen by higher Ca2+ content and mitochondrial membrane depolarization. Moreover, mitochondrial Ca2+ overload contributes to the expression of CD69 via mROS production. Lastly, results suggest that during BCR-dependent activation, mitochondria take up Ca2+ to increase mROS production that can alter the signaling cascade. The in vitro model generated herein, should allow for further exploration of whether mitochondrial Ca2+ overload impacts B cell differentiation.