Ciencias Exactas y Ciencias de la Salud
Permanent URI for this collectionhttps://hdl.handle.net/11285/551039
Pertenecen a esta colección Tesis y Trabajos de grado de las Maestrías correspondientes a las Escuelas de Ingeniería y Ciencias así como a Medicina y Ciencias de la Salud.
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- Engineered mesoporous silica nanoparticles for the co-delivery of quercetin and resveratrol: structural characterization and assessment of antioxidant and anti-inflammatory potential(Instituto Tecnológico y de Estudios Superiores de Monterrey, 2025-12-02) Torres Copado, Andrea; Paul, Sujay; mtyahinojosa, emipsanchez; Arvizu Espinosa, María Goretti; Sahare, Padmavati; School of Engineering and Sciences; Campus Monterrey; Estévez González, Miriam RocíoThe global burden of noncommunicable diseases (NCDs) is closely associated with persistent oxidative stress and chronic inflammation. Natural polyphenols such as quercetin and resveratrol possess potent antioxidant and anti-inflammatory activities; however, their therapeutic potential is severely hindered by low aqueous solubility, poor chemical stability, and rapid metabolic degradation. Nanotechnology-based delivery systems offer a promising approach to enhance the bioavailability and functional performance of these bioactive compounds. Accordingly, this work aimed to co-encapsulate quercetin and resveratrol into mesoporous silica nanoparticles (MSNs), thoroughly characterize the resulting nanocarrier system, and assess its biological properties in vitro. MSNs were synthesized through a modified Stöber method, yielding uniform, spherical, amorphous nanoparticles with an average hydrodynamic diameter of ~126 nm, a high specific surface area (200.3 m²/g), a pore volume of 0.445 cm³/g, and a mean pore diameter of 5.4 nm. Co-loading was achieved using a solvent evaporation method, resulting in high encapsulation efficiencies (79.9% for quercetin and 71.4% for resveratrol). Physicochemical characterization (FTIR, XRD, TGA, DLS, Zeta Potential) confirmed successful drug incorporation, partial amorphization of the polyphenols, enhanced thermal stability, and a sustained release profile extending to 75 hours. The QUE-RES-SiO₂ formulation demonstrated significantly enhanced antioxidant capacity in DPPH, CUPRAC, and ABTS assays, surpassing free resveratrol. Strong anti-inflammatory capacity was also observed in a heat-induced protein denaturation model, with up to 75% inhibition, comparable to free quercetin and the reference drug diclofenac. In ovarian adenocarcinoma SKOV-3 cells, the formulation exhibited efficient nanoparticle uptake; however, it did not induce cytotoxicity or reactive oxygen species (ROS) production within 24 hours, likely due to slow-release kinetics, intrinsic chemoresistance of the cell line, and low concentrations tested over a limited time. Overall, these results demonstrate that MSNs constitute an effective platform for the co-delivery of quercetin and resveratrol, enhancing their stability and antioxidant and anti-inflammatory potential while overcoming key physicochemical limitations. Although anticancer effects were not observed under the tested conditions, this study establishes a robust foundation for future optimization of release kinetics, dosing strategies, and targeting mechanisms to exploit the therapeutic potential of polyphenols in oxidative stress- and inflammation-driven chronic diseases.
- Nanoencapsulation of epigallocatechin-3-gallate in silk fibroin nanoparticles to improve its activity(Instituto Tecnológico y de Estudios Superiores de Monterrey, 2024-05-30) Galindo Martinez, Xochitl Mayte; Mayolo Deloisa, Karla Patricia; emimmayorquin; Jacobo Velázquez, Daniel Alberto; Lozano García, Omar; Benavides Lozano, Jorge Alejandro; Köber, Mariana; Escuela de Ingeniería y Ciencias; Campus Monterrey; Sánchez Trasviña, CalefThe global obesity epidemic represents a significant health challenge, leading to various metabolic disorders and chronic diseases. Current treatments often involve drugs with limited efficacy and significant side effects. Epigallocatechin-3-gallate (EGCG), a polyphenol found in green tea, has demonstrated potential anti-obesogenic effects. However, its low bioavailability and stability limit its therapeutic applications. This study addresses these limitations by exploring the ncapsulation of EGCG in silk fibroin nanoparticles (SFNPs) to enhance its stability and bioactivity. The reverse microemulsion technique was employed to synthesize SFNPs, which were then loaded with EGCG using an adsorption method. The nanoparticles were characterized for their physicochemical properties, including size, polydispersity index (PDI), and zeta potential (ZP). Scanning electron microscopy (SEM) and Fourier-transform infrared spectroscopy (ATR-FTIR) were used to observe morphology and confirm EGCG encapsulation. The antioxidant capacity was evaluated using the ABTS assay, and cell viability was assessed using the Alamar Blue test on the 3T3-L1 cell line. The synthesized EGCG-loaded SFNPs showed slightly increased particle sizes with a low PDI, indicating homogeneous distribution. The zeta potential of EGCG-SFNPs increased compared to SFNPs alone, suggesting modified surface characteristics and potential stability improvement. EGCG retained its antioxidant activity after encapsulation, with increased radical scavenging activity at a 1:10 EGCG-SFNPs mass ratio. Cell viability assays indicated that EGCG-SFNPs were less cytotoxic than SFNPs alone, highlighting the EGCG potential to mitigate adverse effects. The encapsulation efficiency of EGCG in SFNPs was highest at a 1:10 mass ratio, primarily due to the gallate group's affinity for hydrogen bonding and hydrophobic interactions with the nanoparticles. This study demonstrates the potential of EGCG- SFNPs to enhance the stability and bioactivity of EGCG, offering a promising therapeutic strategy for obesity treatment. The findings suggest that encapsulated EGCG could serve as an effective anti-obesogenic agent highlighting the benefits of nanoparticle-based delivery systems in improving the efficacy of bioactive compounds.
- Study on the efficacy of association and internalization of biopolymeric NPs functionalized with exendin-4 on pancreatic islets(Instituto Tecnológico y de Estudios Superiores de Monterrey, 2022-08-30) Contreras Sánchez, Azazel Monserrat; Lozano García, Omar; emipsanchez; García Rivas, Gerardo de Jesús; Ramón Azcón, Javier; Cholula Díaz, Jorge Luis; School of Medicine and Health Sciences; Campus Monterrey; Alves Figueiredo, Hugo JorgePharmacological treatments of diabetes mellitus have the main purpose of maintaining glucose levels and enhancing metabolism, due that most of the drugs are taken orally, they present limitations in delivery specificity. Thus, there is a necessity to develop novel strategies for drug delivery systems based on nanotechnology. Among these, nanoparticles (NPs), have emerged as a promising candidate to improve the delivery by prolonging drug half-life time, and regulating their kinetics release. Furthermore, incorporating a homing peptide to these NPs increases the interaction with the biological system by promoting target specificity. In this work, we developed and optimized NPs based on PLGA and PEG. These NPs were functionalized with a homing peptide drug for pancreatic islets, exendin-4 (Ex-4). Results from hydrodynamic diameter of NPs-Ex-4 at different molar ratio ranged 199.06 ± 50.10 nm with a negative zeta potential. The yield for Ex-4 functionalization with different molar ratio, 1: 0.5 and 1:1, resulted in 0.57 ± 0.28 % and 12.08 ± 3.20%, respectively. Being the molar ratio 1:1 the best protocol for synthesis. The presence of Ex-4 in the surface of the NPs was confirmed by 𝐻 1𝑁𝑀𝑅 spectra. For In vitro studies, FITC was encapsulated in NPs/NPs-Ex-4 to analyze the association and internalization in pancreatic islets. Results demonstrated that there are statistically significant differences of association of NPs/NPs-Ex-4, with a higher association observed in NPs-Ex-4, resulting in a in 31.6 ± 1.36 % of FITC-positive cells to the total area of the islet. The results suggest NPs can internalize into the islet cells after 24 hrs of administration. On the other hand, NPs-Ex-4 showed an incretin effect on insulin secretion associated with the activation of the receptor GLP-1, which activates PKA signaling pathway. This approach emphasizes the concept of multidrug delivery system with targeting capabilities to target pancreatic islets.