Ciencias Exactas y Ciencias de la Salud
Permanent URI for this collectionhttps://hdl.handle.net/11285/551039
Pertenecen a esta colección Tesis y Trabajos de grado de las Maestrías correspondientes a las Escuelas de Ingeniería y Ciencias así como a Medicina y Ciencias de la Salud.
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- Evaluation of the contribution of maize germ peroxidases to resistance to the storage pest prostephanus truncatus(Instituto Tecnológico y de Estudios Superiores de Monterrey, 2025-07) Cervantes Macedo, Elizabeth; García Lara, Silverio; emipsanchez; School of Engineering and Sciences; Campus Monterrey; González Rodríguez, América TzitzikiMaize is a globally important crop used for food, feed, and bioethanol production. However, during storage, it faces serious threats from biotic factors, such as insect pests, particularly Prostephanus truncatus, which can cause severe grain damage and losses of up to 80%, depending on the region and conditions. These infestations also affect grain quality, nutritional value, and marketability. Maize kernels exhibit various resistance mechanisms involving structural, genetic, and biochemical traits, including proteins like peroxidases. Recent studies have linked peroxidase activity to insect resistance, with activity detected in kernel tissues, including the aleurone layer, pedicel, and germ. Although peroxidase activity is mainly concentrated in the germ, little is known about the specific peroxidases involved and their role during insect interactions, creating a gap in our understanding of this defense mechanism. This research aims to analyze the role of germ peroxidases in resistance to P. truncatus in mature maize kernels and germs. Susceptibility of maize kernels and germ tissues to P. truncatus was evaluated and compared with soluble protein concentration and peroxidase activity in the germ. Additionally, a proteomic approach was employed to identify candidate peroxidases and other proteins potentially involved in resistance mechanisms. Results revealed a significant negative correlation between susceptibility traits in maize kernels and peroxidase activity in the germ, as well as a similar correlation between these traits and soluble protein concentration. These findings suggest that peroxidase activity and soluble protein accumulation in the germ likely play an important role in kernel resistance. Despite previous reports of some genotypes exhibiting low germ damage after insect interaction, all germ samples in this study showed relatively high levels of damage, regardless of genotype. Therefore, germ tissue does not exhibit any antixenosis effect. Further research is recommended to evaluate protein candidates in the germ and kernel contributing to resistance during storage.