Ciencias Exactas y Ciencias de la Salud
Permanent URI for this collectionhttps://hdl.handle.net/11285/551039
Pertenecen a esta colección Tesis y Trabajos de grado de las Maestrías correspondientes a las Escuelas de Ingeniería y Ciencias así como a Medicina y Ciencias de la Salud.
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- Synthesis and obtention of CaSiO3 and WO3 ceramic particles as reinforcing fillers of Poly(vinyl alcohol)/Gelatin hydrogels for cartilage regeneration(Instituto Tecnológico y de Estudios Superiores de Monterrey, 2022-06-01) Catzim Ríos, Kevin Stalin; ORTEGA LARA, WENDY DE LOURDES; 95211; Ortega Lara, Wendy de Lourdes; puelquio, emipsanchez; Melo Máximo, Dulce Viridiana; López Mena, Edgar René; Soria Hernández, Cintya Geovanna; School of Engineering and Sciences; Campus Monterrey; Soria Hernández, Cintya GeovannaCartilage wear is a problem that affects a large percentage of the population and has gained relevance in recent years. However, current treatments do not present optimal results that favor the quality of life of those affected. Research in this field has recently focused on the development of systems that promote tissue regeneration instead of replacing it. In this work, the synthesis of CaSiO3 and WO3 ceramic nanoparticles was studied using chemical methods such as sol-gel and precipitation respectively, to later be used as reinforcement of hydrogels composed of poly(vinyl-alcohol)/Gelatin (PVA/Gel) for the improvement of hydrogel bioactivity within biological systems. For the CaSiO3 synthesis, a single pure crystalline phase was obtained, with average particle sizes between 40 and 150 nm. On the other hand, for the WO3 particles, average sizes of 130 nm were obtained. Both independent nanoparticle syntheses were characterized by XRD, SEM, FTIR, DLS and EDX. Viability assays revealed that the hydrogel formulation lowers cell viability by at least 50% in fibroblasts (NIH) and osteoblasts (HFOB). However, silicon-rich particles were found to help improve viability, promoting cell proliferation. Finally, a new non-commercial printing system was developed for freeze-thaw crosslinked hydrogels, where the possibility of 3D printing the generated PVA/Gel formulation was verified.
- Development of surface modified PLGA nanoparticles with a homing peptide for enhanced particle-cell nanobiointeraction(Instituto Tecnológico y de Estudios Superiores de Monterrey, 2021-12) Flores Hernández, Héctor Eduardo; Lozano García, Omar; puelquio/mscuervo; García Rivas, Gerardo de Jesús; Vega Cantú, Yadira Itzel; Aguirre Soto, Héctor Alan; Escuela de Ingeniería y Ciencias; Campus MonterreyNanomaterials presents unique physicochemical, optical, and mechanical properties that are related with the large surface area to mass ratio. During the last 20 years nanotechnology have been related with the sciences of life as an alternative to solve problems using novel strategies that are developed based on nanostructures to reach specific targets in the body. Drug delivery of compounds loaded in nanovehicles has become in a special topic of nanomedicine, in which encapsulated compounds in nanomaterials could reach higher yields, due to the protection from degradation or targeting tissue in in vivo models. However, in in vitro models drug delivery in nanostructured materials has been used to develop different proof of concept to boost novels ideas that can be eventually applied in in vivo models. In this work, we study the surface functionalization method of PLGA nanoparticles using different methods of PEGylation for the further modification surface with Ang II. For PVA coated PLGA nanoparticles we obtain an average hydrodynamic diameter for all nanoformulations around 130 nm and negative surface charge, the yield for PEGylation of nanoparticles and surface modification of Ang II resulted undetectable for almost all methods used for characterization (1H-NMR, FTIR and Lowry method of Ang II detection only) and the biologic interactions with cardiac cells of Ang II surface modified PLGA nanoparticles did not present changes regard to non-surface modified PLGA nanoparticles. In contrast, PLGA-b-PEG nanoparticles present higher hydrodynamic diameter (150 to 225 nm) and negative surface charge, however, the surface modification using Ang II resulted in higher yields that can be characterized and quantified (13.31 mg of Ang/mg of surface PEG). As we expected the biologic interactions with cardiac cells resulted in higher association of PLGA-b-PEG-Ang II nanoparticles than the unmodified PLGA-b-PEG nanoparticles.
- Synthesis of carbon nanotubes on carbon-based structures through the use of nanoparticles, pyrolysis, and chemical vapor deposition(Instituto Tecnológico y de Estudios Superiores de Monterrey, 2021-11-24) Potes Lesoinne, Humberto André; GALLO VILLANUEVA, ROBERTO CARLOS; 213295; Gallo Villanueva, Roberto Carlos; puemcuervo; Pérez González, Victor Hugo; Salazar Soto, Arnoldo; Martínez Chapa, Sergio Omar; School of Engineering and Sciences; Campus Monterrey; Bautista Flores, ClaudiaThe survival of living beings, including humanity, depends on a continuous supply of clean water. However, due to the development of industry, agriculture, and population growth, an increasing number of wastewaters is discarded, and the negative effects of such actions are clear. The first step in solving this situation is the collection and monitoring of pollutants in water bodies to subsequently facilitate their treatment. Nonetheless, traditional sensing techniques are typically laboratory-based, leading to a potential decrease in analysis quality. This work is divided in two main components: a review of recent developments in the micro- and nano- scale electrochemical devices for pollutant detection in wastewater, and the development of a glassy carbon/carbon nanotubes (CNTs) microstructure from SU-8 and iron oxide nanoparticles. The diameter of the produced carbon posts remains constant, at 20 μm, throughout the process, and the produced CNTs of varying lengths have diameters ranging from 500 to 600 nm. The fabrication of these low-cost microstructures requires several steps including photolithography, pyrolysis, and chemical vapor deposition. The addition of CNTs to the carbon-based structure visually increased its surface area and has the potential of enhancing its electrochemical properties.