Ciencias Exactas y Ciencias de la Salud
Permanent URI for this collectionhttps://hdl.handle.net/11285/551039
Pertenecen a esta colección Tesis y Trabajos de grado de las Maestrías correspondientes a las Escuelas de Ingeniería y Ciencias así como a Medicina y Ciencias de la Salud.
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- Postbiotics from cheese whey: Inactivation methods and biological activity of selected microorganisms(Instituto Tecnológico y de Estudios Superiores de Monterrey, 2024-12-02) Osuna Orozco, Francisco Raúl; García Cayuela, Tomás; emipsanchez; Carrillo Nieves, Danay; García Gamboa, Ricardo; Binetti, Ana G.; School of Engineering and Sciences; Campus MonterreyA postbiotic is defined as a preparation of inanimate microorganisms and/or their components that confer a health benefit to the host, with potential applications for modulating the gut microbiota and managing metabolic diseases. While still understudied, postbiotics are gaining attention for their therapeutic possibilities, particularly due to their longer shelf life and safety profile compared to other biotic options. Many food industries are reusing by-products to create functional food ingredients; however, about half of the cheese whey produced by the cheese industry is still discarded untreated, contributing to significant land and water pollution. This study explores cheese whey as a matrix for producing postbiotics through three inactivation methods: pasteurization, thermal sterilization, and ultrasound inactivation. Four microorganisms were selected and tested, including two commercial probiotics (Lactobacillus acidophilus LA3 and Lactobacillus acidophilus LA5); and two strains from the BIOTEC collection (Kluyveromyces lactis BIOTEC009 and Lentilactobacillus kefiri BIOTEC013) isolated from artisanal milk kefir. The fermentative behavior of these microorganisms in cheese whey was analyzed, and the antioxidant, anti-diabetic, and anti-inflammatory properties of the postbiotic preparations were evaluated through several assays and compared to a control group of the same microorganisms without prior inactivation. These results indicated that the postbiotic preparations exhibited desirable properties, often matching or surpassing the bioactivity of live preparations. The thermal sterilization treatment seems to be especially noteworthy, as it managed to enhance the antioxidant capacity of all strains, while the ultrasound treatment seemed favorable for anti-inflammatory properties. Lactobacillus acidophilus LA3 stood out as the strain with the best bioactivity overall, even with its treatments, although, there was no single combination of strain and treatment that was the best for every assay. A techno-economic analysis was carried out by doing a simulation of an industrial- scale process to produce postbiotics using two inactivation methods, pasteurization and sterilization. The analysis concluded that for the parameters established, the project is environmentally friendly and both scenarios can be financially viable with a payback time of the initial investment of 4-5 years.
- Using chaotic printing to fabricate environments that support the culture of requisitive bacteria strains and bacterial communities(Instituto Tecnológico y de Estudios Superiores de Monterrey, 2023) Solís Pérez, Oscar Emmanuel; Santiago, Grissel Trujillo de; mtyahinojosa, emipsanchez; Morones Ramírez, José Rubén; Álvarez, Claudio; Ceballos González, Carlos Fernando; School of Engineering and Sciences; Campus Monterrey; Álvarez, Mario MoisésThis thesis explores the use of 3D bioprinting technologies in enhancing the efficacy and resilience of different bacterial strains contained within the human gut microbiota. By encapsulating these different bacterial species in hydrogel fibers, the study aims to create tailored bacterial mono and co-cultures that overcome the limitations of standard probiotic formulation and delivery, such as susceptibility to environmental stressors and poor colonization capacities while also addressing challenges in the generation and culture of bacterial consortia such as the adequate proliferation of all the members in the community. Our research demonstrates that chaotic bioprinting offers a novel platform for the high-throughput culture and production of probiotics. We successfully cultivated Bifidobacterium bifidum in bioprinted alginate fibers, achieving a viability of 1x1012 CFU/g of fiber within the first 8 hours post-printing, without incubation under a strict anaerobic atmosphere. This highlights the potential of 3D bioprinting in maintaining probiotic viability and enhancing their therapeutic benefits. Furthermore, the study delves into the spatial and growth dynamics of mixed and spatially arranged bacterial communities. By examining communities comprising Escherichia coli, Lactobacillus rhamnosus, and B. bifidum, we demonstrate how chaotic advection influences the arrangement and development of these species. Our findings reveal that the spatial arrangement of bacterial species plays a crucial role in their resilience and development within the community, offering insights into optimizing probiotic formulations and in vitro study models for improved human health benefits. This work contributes to the blooming field of microbiome science; our research paves the way for more effective and resilient probiotic treatments and better understanding the interactions occurring within the microbial world.