Ciencias Exactas y Ciencias de la Salud
Permanent URI for this collectionhttps://hdl.handle.net/11285/551039
Pertenecen a esta colección Tesis y Trabajos de grado de las Maestrías correspondientes a las Escuelas de Ingeniería y Ciencias así como a Medicina y Ciencias de la Salud.
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- Engineered mesoporous silica nanoparticles for the co-delivery of quercetin and resveratrol: structural characterization and assessment of antioxidant and anti-inflammatory potential(Instituto Tecnológico y de Estudios Superiores de Monterrey, 2025-12-02) Torres Copado, Andrea; Paul, Sujay; mtyahinojosa, emipsanchez; Arvizu Espinosa, María Goretti; Sahare, Padmavati; School of Engineering and Sciences; Campus Monterrey; Estévez González, Miriam RocíoThe global burden of noncommunicable diseases (NCDs) is closely associated with persistent oxidative stress and chronic inflammation. Natural polyphenols such as quercetin and resveratrol possess potent antioxidant and anti-inflammatory activities; however, their therapeutic potential is severely hindered by low aqueous solubility, poor chemical stability, and rapid metabolic degradation. Nanotechnology-based delivery systems offer a promising approach to enhance the bioavailability and functional performance of these bioactive compounds. Accordingly, this work aimed to co-encapsulate quercetin and resveratrol into mesoporous silica nanoparticles (MSNs), thoroughly characterize the resulting nanocarrier system, and assess its biological properties in vitro. MSNs were synthesized through a modified Stöber method, yielding uniform, spherical, amorphous nanoparticles with an average hydrodynamic diameter of ~126 nm, a high specific surface area (200.3 m²/g), a pore volume of 0.445 cm³/g, and a mean pore diameter of 5.4 nm. Co-loading was achieved using a solvent evaporation method, resulting in high encapsulation efficiencies (79.9% for quercetin and 71.4% for resveratrol). Physicochemical characterization (FTIR, XRD, TGA, DLS, Zeta Potential) confirmed successful drug incorporation, partial amorphization of the polyphenols, enhanced thermal stability, and a sustained release profile extending to 75 hours. The QUE-RES-SiO₂ formulation demonstrated significantly enhanced antioxidant capacity in DPPH, CUPRAC, and ABTS assays, surpassing free resveratrol. Strong anti-inflammatory capacity was also observed in a heat-induced protein denaturation model, with up to 75% inhibition, comparable to free quercetin and the reference drug diclofenac. In ovarian adenocarcinoma SKOV-3 cells, the formulation exhibited efficient nanoparticle uptake; however, it did not induce cytotoxicity or reactive oxygen species (ROS) production within 24 hours, likely due to slow-release kinetics, intrinsic chemoresistance of the cell line, and low concentrations tested over a limited time. Overall, these results demonstrate that MSNs constitute an effective platform for the co-delivery of quercetin and resveratrol, enhancing their stability and antioxidant and anti-inflammatory potential while overcoming key physicochemical limitations. Although anticancer effects were not observed under the tested conditions, this study establishes a robust foundation for future optimization of release kinetics, dosing strategies, and targeting mechanisms to exploit the therapeutic potential of polyphenols in oxidative stress- and inflammation-driven chronic diseases.
- Fabrication and characterization of microalgae extract loaded chitosan/alginate-based nanoparticles with ultraviolet protection features(Instituto Tecnológico y de Estudios Superiores de Monterrey, 2022-12-06) Santiesteban Romero, Berenice; Iqbal, Hafiz Muhammad Nasir; puemcuervo, emipsanchez; Martínez Ruiz, Manuel; Coronado Apodaca, Karina Guadalupe; Gámez Méndez, Ana María; School of Engineering and Sciences; Campus Monterrey; Sosa Hernández, Juan EduardoMicroalgae are constantly exposed to ultraviolet rays because they are usually found in areas with high sunlight exposure. Several studies have addressed the harmful impact of increased ultraviolet radiation on various microalgae. However, some microalgae have a great ability to overcome or counteract different stresses such as intense solar ultraviolet radiation, this has been happening over the years and with the evolution of microalgae, some examples of defense and/or tolerance mechanisms are DNA repair, synthesis of antioxidants, and enzymatic/non-enzymatic compounds, such as mycosporine-like amino acids and scytonemin to counteract the harmful effects of ultraviolet radiation. This characteristic has not only been investigated to learn more about microalgae, but also for applications such as product creation, since they grow rapidly and are considered the most promising and sustainable sources of biomass. Due to their performance under optimal conditions, they have been widely studied for different bioproducts. Which is why microalgae extracts, by showing resistance to both types of rays, show great potential as sunscreens and this gives a very strong advantage to future bioproducts. This study deals with the application of microalgae extracts for the creation of polymeric nanoparticles based on chitosan and alginate. The synthesis of nanoparticles with a size of 500 nm and a polydispersity index of 0.1 was successfully achieved, indicating the uniformity of these nanoparticles. Different characterization techniques were used for the analysis of these nanoparticles, including UV-Visible spectrophotometry (UV-Vis), Dynamic Light Scattering (DLS), Fourier Transform Infrared Spectroscopy (FTIR) and Scanning Electron Microscope (SEM). All these techniques allow us to see different aspects of the nanoparticles confirming their structure, uniformity, and composition for further effective utilization.