Ciencias Exactas y Ciencias de la Salud
Permanent URI for this collectionhttps://hdl.handle.net/11285/551039
Pertenecen a esta colección Tesis y Trabajos de grado de las Maestrías correspondientes a las Escuelas de Ingeniería y Ciencias así como a Medicina y Ciencias de la Salud.
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- From alpha to beta: biofabricating a pancreatic-like tissue(Instituto Tecnológico y de Estudios Superiores de Monterrey, 2024-05) Méndez Zúñiga, Jair; Moisés Álvarez, Mario; emimmayorquin; Bolívar Monsalve, Edna Johana; Martínez Chapa, Sergio Omar; Escuela de Ingeniería y Ciencias; Campus Monterrey; Trujillo de Santiago, GrisselThis thesis explores the use of genetic engineering of pancreatic α cells and chaotic bioprinting to develop a pancreatic-like tissue capable of expressing transientnd functional insulin. Since there is not an approach to study or treat type 1 diabetes that merge genetic engineering and bioprinting, we addressed this gap and created this thesis. We successfully develop a novel technique to transfect pancreatic α cells called 3D Lipotransfection, as there is not information regarding this topic on α cells, we took this opportunity and lack of information to contributed to the genetic engineering knowledge. The use of 3D Lipotransfection gave us an improvement in comparison with conventional methods, going from just 32% of efficacy using conventional 2D Lipotransfection, to 81% of efficacy for using 3D Lipotransfection at 72 h post treatment. Insulin-producing α cells demonstrated an outstanding performance to several-dose of glucose stimulation and showed an autocrine regulation to it. The increment of efficacy and cellular confluency of 3D Lipotransfection guided us to a better bioprinted tissue, since the number of cells used is of critical importance in this technique. The utilization of chaotic bioprinting in biological contexts has emerged from a desire to address existing challenges in biofabrication and tissue engineering. Our research demonstrates that chaotic bioprinting and 3D Lipotransfection offers a novel platform for the high-throughput cell culture and genetically modified cells’ maintenance. This work contributes to the initiation of the merge of separated fields and proving better results when this is done.
- Biofabrication of nanoenhanced hydrogel fibers for muscle tissue engineering using surface chaotic flows: Chaotic 2D-printing(Instituto Tecnológico y de Estudios Superiores de Monterrey, 2020) Frías Sánchez, Ada Itzel; TRUJILLO DE SANTIAGO, GRISSEL; 256730; FRIAS SANCHEZ, ADA ITZEL; 887018; ALVAREZ, MARIO MOISES; 26048; Trujillo de Santiago, Grissel; RR; Tamayol, Ali; Ponz Ascaso, Fernando; Samandari, Mohamadmahdi; School of Engineering and Sciences; Campus Monterrey; Alvarez, Mario MoisésMultiple human tissues exhibit a fibrous nature. Therefore, the fabrication of hydrogel filaments for biomedical engineering applications is a trending topic. Current tissue models are made of materials that often require further enhancement for appropriate cell attachment, proliferation and differentiation. Here we present a simple strategy, based on the use of mathematically modelable surface chaotic flows, to fabricate continuous, long and thin filaments of gelatin methacryloyl (GelMA) added with Turnip mosaic virus (TuMV) for enhanced muscle tissue engineering. The fabrication of these filaments was achieved by chaotic advection in a finely controlled and miniaturized version of the journal bearing (JB) system. A drop of a pre-gel solution of GelMA was injected on a higher-density viscous fluid (glycerin) and a chaotic flow was applied through an iterative process. The hydrogel drop exponentially deformed and elongated to generate a fiber, which was then photocrosslinked under exposure to UV light. Computational fluid dynamics (CFD) simulations were conducted for the design and prediction of our results. GelMA fibers were then used as scaffolds for C2C12 myoblast cells, and the effect of adding plant-based viral nanoparticles (VNP) to the hydrogel fibers as nano-scaffolds for cellular growth was evaluated. Chaotic 2D-printing was proven to be a viable method for the fabrication of hydrogel fibers. CFD simulations accurately predicted the lengths of the printed fibers, and a correlation coefficient of R2=0.9289 was determined from the experimental and simulated data of the first two cycles. The hydrogel fibers were effective scaffolds for muscle cells and show potential to be used as cost-effective models for muscle tissue engineering purposes. TuMV significantly increased the metabolic activity of the cell-seeded fibers (p<0.05), strengthened cell attachment throughout the first 28 days, improved cell alignment to ~50%, and promoted the generation of structures that resemble natural mammal muscle tissue.