Ciencias Exactas y Ciencias de la Salud

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Pertenecen a esta colección Tesis y Trabajos de grado de las Maestrías correspondientes a las Escuelas de Ingeniería y Ciencias así como a Medicina y Ciencias de la Salud.

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Author: search.filters.author.Flores Loera, Francisco JavierSubject: search.filters.subject.Bioprinting

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    Microbial tale: using chaotic bioprinting to create a structured multi-strain probiotic
    (Instituto Tecnológico y de Estudios Superiores de Monterrey, 2025-06-18) Flores Loera, Francisco Javier; Alvarez, Mario Moises; emipsanchez; Luna Aguirre, Claudia Maribel; Rocha Pizaña, María del Refugio; School of Engineering and Sciences; Campus Monterrey; Trujillo de Santiago, Grissel
    Probiotic therapies offer great potential for addressing gut dysbiosis, but current approaches are limited by low strain diversity, high production costs, and the challenges of culturing strict anaerobes. To overcome these limitations, this work introduces a novel strategy combining based on continuous chaotic bioprinting to create structured (micro-architected) co-cultures of probiotic bacteria. Using a Kenics static mixer–based printhead, we fabricated alginate hydrogel filaments with an internal multilayered microarchitecture containing four probiotic strains: Bifidobacterium bifidum, Bacteroides fragilis, Lactobacillus rhamnosus, and Streptococcus thermophilus. The spatial arrangement of the multilayered architecture was designed to promote cooperative interactions, particularly by embedding strict anaerobes between facultative anaerobes to create self-sustaining hypoxic niches. The printed constructs were characterized over 72 hours using fluorescence microscopy, colony-forming unit counts, LIVE/DEAD assays, and qPCR. Results showed that structured co-cultures exhibited higher viability, enhanced growth, and more balanced population dynamics than the monocultures of each bacterial strain and unstructured (scrambled) co-cultures. This study demonstrates that chaotic bioprinting enables precise spatial control over microbial ecosystems, allowing the rational design of microbial communities with tailored interactions. The approach presents a powerful and scalable platform for next-generation probiotic production and opens new opportunities for engineered microbiomes, synthetic biology, and living material design.
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