Ciencias Exactas y Ciencias de la Salud
Permanent URI for this collectionhttps://hdl.handle.net/11285/551039
Pertenecen a esta colección Tesis y Trabajos de grado de las Maestrías correspondientes a las Escuelas de Ingeniería y Ciencias así como a Medicina y Ciencias de la Salud.
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- Engineered mesoporous silica nanoparticles for the co-delivery of quercetin and resveratrol: structural characterization and assessment of antioxidant and anti-inflammatory potential(Instituto Tecnológico y de Estudios Superiores de Monterrey, 2025-12-02) Torres Copado, Andrea; Paul, Sujay; mtyahinojosa, emipsanchez; Arvizu Espinosa, María Goretti; Sahare, Padmavati; School of Engineering and Sciences; Campus Monterrey; Estévez González, Miriam RocíoThe global burden of noncommunicable diseases (NCDs) is closely associated with persistent oxidative stress and chronic inflammation. Natural polyphenols such as quercetin and resveratrol possess potent antioxidant and anti-inflammatory activities; however, their therapeutic potential is severely hindered by low aqueous solubility, poor chemical stability, and rapid metabolic degradation. Nanotechnology-based delivery systems offer a promising approach to enhance the bioavailability and functional performance of these bioactive compounds. Accordingly, this work aimed to co-encapsulate quercetin and resveratrol into mesoporous silica nanoparticles (MSNs), thoroughly characterize the resulting nanocarrier system, and assess its biological properties in vitro. MSNs were synthesized through a modified Stöber method, yielding uniform, spherical, amorphous nanoparticles with an average hydrodynamic diameter of ~126 nm, a high specific surface area (200.3 m²/g), a pore volume of 0.445 cm³/g, and a mean pore diameter of 5.4 nm. Co-loading was achieved using a solvent evaporation method, resulting in high encapsulation efficiencies (79.9% for quercetin and 71.4% for resveratrol). Physicochemical characterization (FTIR, XRD, TGA, DLS, Zeta Potential) confirmed successful drug incorporation, partial amorphization of the polyphenols, enhanced thermal stability, and a sustained release profile extending to 75 hours. The QUE-RES-SiO₂ formulation demonstrated significantly enhanced antioxidant capacity in DPPH, CUPRAC, and ABTS assays, surpassing free resveratrol. Strong anti-inflammatory capacity was also observed in a heat-induced protein denaturation model, with up to 75% inhibition, comparable to free quercetin and the reference drug diclofenac. In ovarian adenocarcinoma SKOV-3 cells, the formulation exhibited efficient nanoparticle uptake; however, it did not induce cytotoxicity or reactive oxygen species (ROS) production within 24 hours, likely due to slow-release kinetics, intrinsic chemoresistance of the cell line, and low concentrations tested over a limited time. Overall, these results demonstrate that MSNs constitute an effective platform for the co-delivery of quercetin and resveratrol, enhancing their stability and antioxidant and anti-inflammatory potential while overcoming key physicochemical limitations. Although anticancer effects were not observed under the tested conditions, this study establishes a robust foundation for future optimization of release kinetics, dosing strategies, and targeting mechanisms to exploit the therapeutic potential of polyphenols in oxidative stress- and inflammation-driven chronic diseases.
- Therapeutic potential of glucose oxidase loaded mesoporous silica nanoparticles in ovarian cancer(Instituto Tecnológico y de Estudios Superiores de Monterrey, 2025-06-12) Urióstegui Peña, Andrea Georgina; Paul, Sujay; emipsanchez; Sahare, Padmavati; School of Engineering and Sciences; Campus Monterrey; Luna Bárcenas, GabrielOvarian cancer (OC) represents ones of the most dangerous malignancies of the female reproductive system, claiming the lives of thousands of women worldwide. Rapid disease progression and non-efficient treatments that cause systemic toxicity offer opportunities for innovative technology to address these issues. Glucose oxidase (GOx) is an enzyme that converts glucose into D-gluconic acid and hydrogen peroxide (H2O2), thus being used as a starvation strategy for cancer cells. However, systemic toxicity, inadequate stability, and immunogenicity hinder the application of GOx in cancer therapy. Nanotechnology surges as a strategy to improve therapeutic efficacy while minimizing side effects when used as drug delivery systems (DDS). Mesoporous silica nanoparticles (MSNs) serve as nanocarriers owing to their elevated loading capacity, facile functionalization, extensive surface area, and biocompatibility. This study seeks to examine the impact of GOx-loaded MSNs in OC by assessing cytotoxicity and differential expression of genes associated with carcinogenesis. The immobilization of GOx onto MSNS was conducted using 3-aminopropiltrietoxysilane (APTES) and glutaraldehyde (GTA). This approach yielded an immobilization percentage of 49.24%, accompanied by a reduction in enzymatic activity in the nanoformulation relative to the free enzyme. Techniques such as FTIR, DLS, SEM/EDX, XRD and BET were employed to characterize the MSNs before and after their immobilization with GOx. In vitro cytotoxicity and target gene expression were evaluated in the SKOV3 cell line. The IC50 values for free and immobilized GOx were found to be 60.77 ng/mL and 111.6 μg/mL, respectively. Moreover, a significant downregulation of the oncogene CTNNB1 was observed after 24 h of treatment with the nanoformulation. These findings represent the initial advancements in the application of GOx for ovarian cancer treatment.
- Evaluation of the Synergistic Effects of Curcumin-Resveratrol co-loaded Mesoporous Silica Nanoparticles on Colorectal Cancer Cells(Instituto Tecnológico y de Estudios Superiores de Monterrey, 2023-12-04) Ochoa Sánchez, Adriana; Paul, Sujay; emimmayorquin; Arvizu Aguilar, María Goretti; EIC Escuela de Ingeniería y Ciencias; Campus Monterrey; Estévez González, Miriam RocíoColorectal cancer (CRC) poses a substantial global health challenge, necessitating innovative solutions due to the limitations inherent in current conventional treatments. Curcumin and resveratrol, natural phytochemicals, have emerged as promising anticancer agents, offering therapeutic benefits with minimal toxicity to healthy tissues. However, the clinical application of these phytochemicals faces limitations. Nanoformulations have gained prominence for their ability to overcome these constraints, ensuring improved solubility, controlled release, and enhanced biocompatibility. Mesoporous silica nanoparticles (MSNs) have garnered attention as nanocarriers due to their exceptional biocompatibility and high load capacity. This study focused on investigating the effect of co-loaded curcumin and resveratrol MSNs on target gene expression, which is crucial in cancerogenesis. Curcumin and resveratrol were immobilized onto MSNs functionalized with 3- aminopropyltriethoxysilane (APTES) and glutaraldehyde (GTA). The co-loaded MSNs were characterized using FTIR, TGA, SEM, XRD, BET, and determination of encapsulation efficiency by UV-Visible spectrophotometry. In vitro cytotoxicity assays were conducted using HCT-116 and Caco-2 colorectal cell lines. Target gene expression was evaluated through RT-qPCR after treating HCT-116 cancer cells with the nanoformulation at 24 and 48-hour time points. The results revealed significant upregulation of the tumor suppressor gene TP53 and apoptosis-related gene Bax, coupled with downregulation of proliferation-related genes Wnt-1 and CTNNB1. These findings demonstrate the ability of co-loaded curcumin and resveratrol MSNs to modulate key genes involved in cancer progression, showcasing their potential as a promising therapeutic strategy for CRC.
- The effect of thymoquinone on key onco- and tumor suppressor miRNAs in HCT-15 colorectal cancer cell line(Instituto Tecnológico y de Estudios Superiores de Monterrey, 2023-07-27) Estrada Meza, Carolina; Paul, Sujay; dnbsrp; Arvizu-Espinosa, María Goretti; Escuela de Ingeniería y Ciencias (EIC); Campus Monterrey; Sharma, AshutoshCancer still a major global health concern and stands as one of the most fatal diseases in modern times, causing a considerable number of deaths each year. Colorectal cancer (CRC) is among the most prevalent and deadly types of cancer in the world. Conventional cancer therapies often come with limitations, including high costs, limited accessibility, and severe side effects. Therefore, exploring alternative treatment approaches is crucial. Phytochemicals, naturally occurring bioactive compounds in plants, have shown promise in reducing the risk of various cancers and may serve as valuable additions to cancer prevention and treatment strategies. This study focused on the potential of thymoquinone (TQ), a compound found in Nigella sativa, as an antiproliferative agent in colon cancer cells. The study investigated the effect of TQ on microRNA (miRNA) expression, which plays a crucial role in regulating gene expression and is implicated in cancer. The antiproliferative activity of TQ was evaluated in HCT-15 colon cancer cells, and the expression pattern of key onco- and tumor suppressor miRNAs such as miR-34a-5p, let-7, miR-200a-5p, and miR-21-5p, as well as common target genes such as PTEN and BCL2 which are commonly dysregulated in cancer, were analyzed. The results highlighted the significant downregulation of miR-21-5p expression in TQ-treated cells, suggesting the potential of TQ to modulate this oncogenic miRNA. Additionally, a time-dependent inhibitory response to miR-21-5p was observed with TQ treatment. These findings support TQ's ability to prevent colon cancer cells from proliferating and its potential for modulating key miRNAs involved in cancer progression. Targeting miRNAs may hold promise as a therapeutic strategy for colon cancer. To better understand the underlying mechanisms and investigate TQ's potential as a therapy option for colon cancer, more study is required. This study represents the first investigation of TQ's effect on the expression profile of miRNAs in colon cancer cells.
- Thymoquinone mediated modulation of key microRNAs in human prostatic adenocarcinoma cells and its therapeutic potential as an anticancer agent(Instituto Tecnológico y de Estudios Superiores de Monterrey, 2023-07-10) Osorio Pérez, Sofía Madeline; Paul, Sujay; emiggomez, emipsanchez; Arvizu Espinosa, María Goretti; Escuela de Ingeniería y Ciencias; Campus Monterrey; Sharma, AshutoshWorldwide, prostate cancer (PC) is among the leading causes of morbidity and mortality in males. Studies have suggested that dietary compounds can play a role in preventing and inhibiting prostate cancer. Thymoquinone (TQ), a phytochemical found in black cumin, has demonstrated various pharmacological properties, including antineoplastic effects. MicroRNAs (miRNAs) regulate gene expression and significantly affect prostate cancer progression. This study aims to investigate the antiproliferative properties of TQ and its influence on significant microRNAs (miRNAs) in PC3 prostate cancer cells. PC3 cells were treated with different concentrations of TQ, and cell viability was assessed using an MTT assay. The treatment revealed a dose-dependent decrease in cell viability with an IC50 of 55.83 μM. Subsequently, the expression levels of miR-34a-5p, miR-221-5p, miR-17-5p, and miR21-5p were analyzed by RT-qPCR. TQ treatment significantly upregulated expression levels of miR-34a-5p (4.45-fold increase, p<0.05), miR-221-5p (2.57-fold), miR-17-5p (11.85-fold), and miR21-5p (10.38-fold). Notably, the upregulation of miR-34a-5p highlights its potential as a therapeutic target for PC. These findings suggest that TQ possesses antiproliferative properties and can modulate miRNA expression in PC3 cells, providing insights into the molecular mechanisms underlying its effects on miRNA profiles in prostate cancer. Further investigations are warranted to fully elucidate the role of TQ in miRNA-mediated prostate cancer pathogenesis.
- Anticancer activity of Elaphoglossum paleaceum (Hook. & Grev.) sledge derived phloroglucinols in human hepatocellular carcinoma cells through potential transcriptional modulation of microRNAs(Instituto Tecnológico y de Estudios Superiores de Monterrey, 2022-01-05) Ruiz Manriquez, Luis Mario; PAUL, SUJAY; 512948; Paul, Sujay; emijzarate/puemcuervo; Arvizu Espinosa, María Goretti; School of Engineering and Sciences; Campus Monterrey; Sharma, AshutoshPhloroglucinols derivatives are an important class of secondary metabolites widely distributed in Pteridophytes of the genera Elaphoglossum that exhibit multiple biological activities, including anticancer activity. Recent evidence suggests that many phytochemicals, including polyphenols, can significantly alter microRNA (miRNAs) expression profiles. miRNAs are short non-coding RNAs that can act as tumor suppressors or onco-miRNAs regulating the expression of cancer-associated genes post-transcriptionally, therefore, modulating cancer progression. Elaphoglossum paleaceum (Hook. & Grev.) Sledge phloroglucinols derivatives have demonstrated cytotoxic activity against several cancer models. Nevertheless, its relative mechanism of action and its effect on HCC cells have not still investigated. The present study aimed to evaluate the anticancer activity of hexanic/chloroformic extract of E. paleaceum and investigate its mechanism(s) of action on tumor suppressor and onco-miRNAs expression involved in HCC carcinogenesis. E. paleaceum hexanic/chloroformic extract exhibited cytotoxicity against HepG2 cells. Moreover, an E. paleaceum hexanic/chloroformic extract concentration approximating the IC50 induced upregulation of tumor suppressors miR-200b-5p, miR-34-5p, Let-7b-5p, and miR-218b-5p. These modulatory effects might contribute to inhibiting HepG2 cell growth observed after treatment with E. paleaceum hexanic/chloroformic extract. These data demonstrate the ability of E. paleaceum phloroglucinols derivatives to impede hepatocellular carcinoma cell proliferation, possibly by modulating critical miRNAs and consequently their downstream targets, suggesting a novel mechanism of the anticancer potential of phloroglucinols derivatives and positioning them as potential novel anticancer agents.