Ciencias Exactas y Ciencias de la Salud
Permanent URI for this collectionhttps://hdl.handle.net/11285/551039
Pertenecen a esta colección Tesis y Trabajos de grado de las Maestrías correspondientes a las Escuelas de Ingeniería y Ciencias así como a Medicina y Ciencias de la Salud.
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- Therapeutic potential of glucose oxidase loaded mesoporous silica nanoparticles in ovarian cancer(Instituto Tecnológico y de Estudios Superiores de Monterrey, 2025-06-12) Urióstegui Peña, Andrea Georgina; Paul, Sujay; emipsanchez; Sahare, Padmavati; School of Engineering and Sciences; Campus Monterrey; Luna Bárcenas, GabrielOvarian cancer (OC) represents ones of the most dangerous malignancies of the female reproductive system, claiming the lives of thousands of women worldwide. Rapid disease progression and non-efficient treatments that cause systemic toxicity offer opportunities for innovative technology to address these issues. Glucose oxidase (GOx) is an enzyme that converts glucose into D-gluconic acid and hydrogen peroxide (H2O2), thus being used as a starvation strategy for cancer cells. However, systemic toxicity, inadequate stability, and immunogenicity hinder the application of GOx in cancer therapy. Nanotechnology surges as a strategy to improve therapeutic efficacy while minimizing side effects when used as drug delivery systems (DDS). Mesoporous silica nanoparticles (MSNs) serve as nanocarriers owing to their elevated loading capacity, facile functionalization, extensive surface area, and biocompatibility. This study seeks to examine the impact of GOx-loaded MSNs in OC by assessing cytotoxicity and differential expression of genes associated with carcinogenesis. The immobilization of GOx onto MSNS was conducted using 3-aminopropiltrietoxysilane (APTES) and glutaraldehyde (GTA). This approach yielded an immobilization percentage of 49.24%, accompanied by a reduction in enzymatic activity in the nanoformulation relative to the free enzyme. Techniques such as FTIR, DLS, SEM/EDX, XRD and BET were employed to characterize the MSNs before and after their immobilization with GOx. In vitro cytotoxicity and target gene expression were evaluated in the SKOV3 cell line. The IC50 values for free and immobilized GOx were found to be 60.77 ng/mL and 111.6 μg/mL, respectively. Moreover, a significant downregulation of the oncogene CTNNB1 was observed after 24 h of treatment with the nanoformulation. These findings represent the initial advancements in the application of GOx for ovarian cancer treatment.
- Evaluation of the Synergistic Effects of Curcumin-Resveratrol co-loaded Mesoporous Silica Nanoparticles on Colorectal Cancer Cells(Instituto Tecnológico y de Estudios Superiores de Monterrey, 2023-12-04) Ochoa Sánchez, Adriana; Paul, Sujay; emimmayorquin; Arvizu Aguilar, María Goretti; EIC Escuela de Ingeniería y Ciencias; Campus Monterrey; Estévez González, Miriam RocíoColorectal cancer (CRC) poses a substantial global health challenge, necessitating innovative solutions due to the limitations inherent in current conventional treatments. Curcumin and resveratrol, natural phytochemicals, have emerged as promising anticancer agents, offering therapeutic benefits with minimal toxicity to healthy tissues. However, the clinical application of these phytochemicals faces limitations. Nanoformulations have gained prominence for their ability to overcome these constraints, ensuring improved solubility, controlled release, and enhanced biocompatibility. Mesoporous silica nanoparticles (MSNs) have garnered attention as nanocarriers due to their exceptional biocompatibility and high load capacity. This study focused on investigating the effect of co-loaded curcumin and resveratrol MSNs on target gene expression, which is crucial in cancerogenesis. Curcumin and resveratrol were immobilized onto MSNs functionalized with 3- aminopropyltriethoxysilane (APTES) and glutaraldehyde (GTA). The co-loaded MSNs were characterized using FTIR, TGA, SEM, XRD, BET, and determination of encapsulation efficiency by UV-Visible spectrophotometry. In vitro cytotoxicity assays were conducted using HCT-116 and Caco-2 colorectal cell lines. Target gene expression was evaluated through RT-qPCR after treating HCT-116 cancer cells with the nanoformulation at 24 and 48-hour time points. The results revealed significant upregulation of the tumor suppressor gene TP53 and apoptosis-related gene Bax, coupled with downregulation of proliferation-related genes Wnt-1 and CTNNB1. These findings demonstrate the ability of co-loaded curcumin and resveratrol MSNs to modulate key genes involved in cancer progression, showcasing their potential as a promising therapeutic strategy for CRC.