Ciencias Exactas y Ciencias de la Salud
Permanent URI for this collectionhttps://hdl.handle.net/11285/551039
Pertenecen a esta colección Tesis y Trabajos de grado de las Maestrías correspondientes a las Escuelas de Ingeniería y Ciencias así como a Medicina y Ciencias de la Salud.
Browse
Search Results
- Engineered mesoporous silica nanoparticles for the co-delivery of quercetin and resveratrol: structural characterization and assessment of antioxidant and anti-inflammatory potential(Instituto Tecnológico y de Estudios Superiores de Monterrey, 2025-12-02) Torres Copado, Andrea; Paul, Sujay; mtyahinojosa, emipsanchez; Arvizu Espinosa, María Goretti; Sahare, Padmavati; School of Engineering and Sciences; Campus Monterrey; Estévez González, Miriam RocíoThe global burden of noncommunicable diseases (NCDs) is closely associated with persistent oxidative stress and chronic inflammation. Natural polyphenols such as quercetin and resveratrol possess potent antioxidant and anti-inflammatory activities; however, their therapeutic potential is severely hindered by low aqueous solubility, poor chemical stability, and rapid metabolic degradation. Nanotechnology-based delivery systems offer a promising approach to enhance the bioavailability and functional performance of these bioactive compounds. Accordingly, this work aimed to co-encapsulate quercetin and resveratrol into mesoporous silica nanoparticles (MSNs), thoroughly characterize the resulting nanocarrier system, and assess its biological properties in vitro. MSNs were synthesized through a modified Stöber method, yielding uniform, spherical, amorphous nanoparticles with an average hydrodynamic diameter of ~126 nm, a high specific surface area (200.3 m²/g), a pore volume of 0.445 cm³/g, and a mean pore diameter of 5.4 nm. Co-loading was achieved using a solvent evaporation method, resulting in high encapsulation efficiencies (79.9% for quercetin and 71.4% for resveratrol). Physicochemical characterization (FTIR, XRD, TGA, DLS, Zeta Potential) confirmed successful drug incorporation, partial amorphization of the polyphenols, enhanced thermal stability, and a sustained release profile extending to 75 hours. The QUE-RES-SiO₂ formulation demonstrated significantly enhanced antioxidant capacity in DPPH, CUPRAC, and ABTS assays, surpassing free resveratrol. Strong anti-inflammatory capacity was also observed in a heat-induced protein denaturation model, with up to 75% inhibition, comparable to free quercetin and the reference drug diclofenac. In ovarian adenocarcinoma SKOV-3 cells, the formulation exhibited efficient nanoparticle uptake; however, it did not induce cytotoxicity or reactive oxygen species (ROS) production within 24 hours, likely due to slow-release kinetics, intrinsic chemoresistance of the cell line, and low concentrations tested over a limited time. Overall, these results demonstrate that MSNs constitute an effective platform for the co-delivery of quercetin and resveratrol, enhancing their stability and antioxidant and anti-inflammatory potential while overcoming key physicochemical limitations. Although anticancer effects were not observed under the tested conditions, this study establishes a robust foundation for future optimization of release kinetics, dosing strategies, and targeting mechanisms to exploit the therapeutic potential of polyphenols in oxidative stress- and inflammation-driven chronic diseases.
- Thymoquinone mediated modulation of key microRNAs in human prostatic adenocarcinoma cells and its therapeutic potential as an anticancer agent(Instituto Tecnológico y de Estudios Superiores de Monterrey, 2023-07-10) Osorio Pérez, Sofía Madeline; Paul, Sujay; emiggomez, emipsanchez; Arvizu Espinosa, María Goretti; Escuela de Ingeniería y Ciencias; Campus Monterrey; Sharma, AshutoshWorldwide, prostate cancer (PC) is among the leading causes of morbidity and mortality in males. Studies have suggested that dietary compounds can play a role in preventing and inhibiting prostate cancer. Thymoquinone (TQ), a phytochemical found in black cumin, has demonstrated various pharmacological properties, including antineoplastic effects. MicroRNAs (miRNAs) regulate gene expression and significantly affect prostate cancer progression. This study aims to investigate the antiproliferative properties of TQ and its influence on significant microRNAs (miRNAs) in PC3 prostate cancer cells. PC3 cells were treated with different concentrations of TQ, and cell viability was assessed using an MTT assay. The treatment revealed a dose-dependent decrease in cell viability with an IC50 of 55.83 μM. Subsequently, the expression levels of miR-34a-5p, miR-221-5p, miR-17-5p, and miR21-5p were analyzed by RT-qPCR. TQ treatment significantly upregulated expression levels of miR-34a-5p (4.45-fold increase, p<0.05), miR-221-5p (2.57-fold), miR-17-5p (11.85-fold), and miR21-5p (10.38-fold). Notably, the upregulation of miR-34a-5p highlights its potential as a therapeutic target for PC. These findings suggest that TQ possesses antiproliferative properties and can modulate miRNA expression in PC3 cells, providing insights into the molecular mechanisms underlying its effects on miRNA profiles in prostate cancer. Further investigations are warranted to fully elucidate the role of TQ in miRNA-mediated prostate cancer pathogenesis.