Ciencias Exactas y Ciencias de la Salud
Permanent URI for this collectionhttps://hdl.handle.net/11285/551039
Pertenecen a esta colección Tesis y Trabajos de grado de las Maestrías correspondientes a las Escuelas de Ingeniería y Ciencias así como a Medicina y Ciencias de la Salud.
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- Engineered mesoporous silica nanoparticles for the co-delivery of quercetin and resveratrol: structural characterization and assessment of antioxidant and anti-inflammatory potential(Instituto Tecnológico y de Estudios Superiores de Monterrey, 2025-12-02) Torres Copado, Andrea; Paul, Sujay; mtyahinojosa, emipsanchez; Arvizu Espinosa, María Goretti; Sahare, Padmavati; School of Engineering and Sciences; Campus Monterrey; Estévez González, Miriam RocíoThe global burden of noncommunicable diseases (NCDs) is closely associated with persistent oxidative stress and chronic inflammation. Natural polyphenols such as quercetin and resveratrol possess potent antioxidant and anti-inflammatory activities; however, their therapeutic potential is severely hindered by low aqueous solubility, poor chemical stability, and rapid metabolic degradation. Nanotechnology-based delivery systems offer a promising approach to enhance the bioavailability and functional performance of these bioactive compounds. Accordingly, this work aimed to co-encapsulate quercetin and resveratrol into mesoporous silica nanoparticles (MSNs), thoroughly characterize the resulting nanocarrier system, and assess its biological properties in vitro. MSNs were synthesized through a modified Stöber method, yielding uniform, spherical, amorphous nanoparticles with an average hydrodynamic diameter of ~126 nm, a high specific surface area (200.3 m²/g), a pore volume of 0.445 cm³/g, and a mean pore diameter of 5.4 nm. Co-loading was achieved using a solvent evaporation method, resulting in high encapsulation efficiencies (79.9% for quercetin and 71.4% for resveratrol). Physicochemical characterization (FTIR, XRD, TGA, DLS, Zeta Potential) confirmed successful drug incorporation, partial amorphization of the polyphenols, enhanced thermal stability, and a sustained release profile extending to 75 hours. The QUE-RES-SiO₂ formulation demonstrated significantly enhanced antioxidant capacity in DPPH, CUPRAC, and ABTS assays, surpassing free resveratrol. Strong anti-inflammatory capacity was also observed in a heat-induced protein denaturation model, with up to 75% inhibition, comparable to free quercetin and the reference drug diclofenac. In ovarian adenocarcinoma SKOV-3 cells, the formulation exhibited efficient nanoparticle uptake; however, it did not induce cytotoxicity or reactive oxygen species (ROS) production within 24 hours, likely due to slow-release kinetics, intrinsic chemoresistance of the cell line, and low concentrations tested over a limited time. Overall, these results demonstrate that MSNs constitute an effective platform for the co-delivery of quercetin and resveratrol, enhancing their stability and antioxidant and anti-inflammatory potential while overcoming key physicochemical limitations. Although anticancer effects were not observed under the tested conditions, this study establishes a robust foundation for future optimization of release kinetics, dosing strategies, and targeting mechanisms to exploit the therapeutic potential of polyphenols in oxidative stress- and inflammation-driven chronic diseases.
- Therapeutic potential of glucose oxidase loaded mesoporous silica nanoparticles in ovarian cancer(Instituto Tecnológico y de Estudios Superiores de Monterrey, 2025-06-12) Urióstegui Peña, Andrea Georgina; Paul, Sujay; emipsanchez; Sahare, Padmavati; School of Engineering and Sciences; Campus Monterrey; Luna Bárcenas, GabrielOvarian cancer (OC) represents ones of the most dangerous malignancies of the female reproductive system, claiming the lives of thousands of women worldwide. Rapid disease progression and non-efficient treatments that cause systemic toxicity offer opportunities for innovative technology to address these issues. Glucose oxidase (GOx) is an enzyme that converts glucose into D-gluconic acid and hydrogen peroxide (H2O2), thus being used as a starvation strategy for cancer cells. However, systemic toxicity, inadequate stability, and immunogenicity hinder the application of GOx in cancer therapy. Nanotechnology surges as a strategy to improve therapeutic efficacy while minimizing side effects when used as drug delivery systems (DDS). Mesoporous silica nanoparticles (MSNs) serve as nanocarriers owing to their elevated loading capacity, facile functionalization, extensive surface area, and biocompatibility. This study seeks to examine the impact of GOx-loaded MSNs in OC by assessing cytotoxicity and differential expression of genes associated with carcinogenesis. The immobilization of GOx onto MSNS was conducted using 3-aminopropiltrietoxysilane (APTES) and glutaraldehyde (GTA). This approach yielded an immobilization percentage of 49.24%, accompanied by a reduction in enzymatic activity in the nanoformulation relative to the free enzyme. Techniques such as FTIR, DLS, SEM/EDX, XRD and BET were employed to characterize the MSNs before and after their immobilization with GOx. In vitro cytotoxicity and target gene expression were evaluated in the SKOV3 cell line. The IC50 values for free and immobilized GOx were found to be 60.77 ng/mL and 111.6 μg/mL, respectively. Moreover, a significant downregulation of the oncogene CTNNB1 was observed after 24 h of treatment with the nanoformulation. These findings represent the initial advancements in the application of GOx for ovarian cancer treatment.
- Anticancer activity of Elaphoglossum paleaceum (Hook. & Grev.) sledge derived phloroglucinols in human hepatocellular carcinoma cells through potential transcriptional modulation of microRNAs(Instituto Tecnológico y de Estudios Superiores de Monterrey, 2022-01-05) Ruiz Manriquez, Luis Mario; PAUL, SUJAY; 512948; Paul, Sujay; emijzarate/puemcuervo; Arvizu Espinosa, María Goretti; School of Engineering and Sciences; Campus Monterrey; Sharma, AshutoshPhloroglucinols derivatives are an important class of secondary metabolites widely distributed in Pteridophytes of the genera Elaphoglossum that exhibit multiple biological activities, including anticancer activity. Recent evidence suggests that many phytochemicals, including polyphenols, can significantly alter microRNA (miRNAs) expression profiles. miRNAs are short non-coding RNAs that can act as tumor suppressors or onco-miRNAs regulating the expression of cancer-associated genes post-transcriptionally, therefore, modulating cancer progression. Elaphoglossum paleaceum (Hook. & Grev.) Sledge phloroglucinols derivatives have demonstrated cytotoxic activity against several cancer models. Nevertheless, its relative mechanism of action and its effect on HCC cells have not still investigated. The present study aimed to evaluate the anticancer activity of hexanic/chloroformic extract of E. paleaceum and investigate its mechanism(s) of action on tumor suppressor and onco-miRNAs expression involved in HCC carcinogenesis. E. paleaceum hexanic/chloroformic extract exhibited cytotoxicity against HepG2 cells. Moreover, an E. paleaceum hexanic/chloroformic extract concentration approximating the IC50 induced upregulation of tumor suppressors miR-200b-5p, miR-34-5p, Let-7b-5p, and miR-218b-5p. These modulatory effects might contribute to inhibiting HepG2 cell growth observed after treatment with E. paleaceum hexanic/chloroformic extract. These data demonstrate the ability of E. paleaceum phloroglucinols derivatives to impede hepatocellular carcinoma cell proliferation, possibly by modulating critical miRNAs and consequently their downstream targets, suggesting a novel mechanism of the anticancer potential of phloroglucinols derivatives and positioning them as potential novel anticancer agents.