Ciencias Exactas y Ciencias de la Salud
Permanent URI for this collectionhttps://hdl.handle.net/11285/551039
Pertenecen a esta colección Tesis y Trabajos de grado de las Maestrías correspondientes a las Escuelas de Ingeniería y Ciencias así como a Medicina y Ciencias de la Salud.
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- Drug delivery dynamics of biopolymer-protein based nanostructures cues via experimental approach and mathematical modeling(Instituto Tecnológico y de Estudios Superiores de Monterrey, 2022-06-07) Ibarra Sánchez, Luis Ángel; IQBAL, HAFIZ MUHAMMAD NASIR; 735340; Nasir Iqbal, Hafiz Muhammad; emipsanchez; Parra Saldívar, Roberto; Melchor Martínez, Elda Madai; Gámez Méndez, Ana María; School of Engineering and Sciences; Campus Monterrey; Sosa Hernández, Juan EduardoRespiratory diseases are leading the burden in public health, usually found in the top chart of leading causes of death for many countries. Moreover, COVID-19 has aggravated this situation, having numerous patients with mild to severe symptoms. Besides, lung tissue inflammation and mucus overproduction are critical factors in patients’ comorbidity, not only for COVID-19 but also in other pulmonary diseases. In this project, the aim was to integrate the natural therapeutics curcumin (with a studied anti-inflammatory effect) and papain (a proteolytic enzyme used for mucus degradation) into a drug delivery nanostructure to administer intranasally. To reach this goal, curcumin was encapsulated in alginate particles with the emulsion-gelation method, obtaining an encapsulation efficiency of 81.23%. Also, curcumin particles showed a mean size of 500.8 nm, and a surface charge of -23.5 mV. Nonetheless, more studies are required to fully understand the emulsion system to obtain smaller and less disperse particles. Also, bioavailability and efficacy test are required to confirm feasibility of the project. In summary, nanoencapsulation in alginate via emulsion-gelation method has shown promising results for enhancing the curcumin solubility, bioavailability, and stability, to develop more efficient pulmonary treatments against inflammation.
- Integrated bio-refinery processes for valorization of Sargassum biomass from Mexican Caribbean Coast(Instituto Tecnológico y de Estudios Superiores de Monterrey, 2020-11-24) Saldarriaga Hernández, Sara Cristina; Nasir Iqbal, Hafiz Muhammad; puelquio/tolmquevedo; Melchor Martínez, Elda M.; Escuela de Ingeniería y Ciencias; Campus Monterrey; Parra Saldívar, RobertoSince 2014, Mexican Caribbean coasts have experienced an atypical massive arrival of pelagic Sargassum that accumulated on the shores triggers economic losses, public health problems, and ecosystem damaging near the coastline. Mechanical harvesting has been implemented ending in landfills. Since Sargassum algae represent an abundant biomass in tropical regions of the world, it has shown potential as a feedstock to supply bioprocesses focused on obtaining high-value compounds and bioproducts. However, there is a lack of data on the biochemical composition of Sargassum biomass from Mexican Caribbean coasts to propose valorization pathways. In this sense we conducted a biochemical and elemental characterization of Sargassum biomass collected from Cozumel, Mexico, and evaluated how different factors such as Season, type of Sargassum and extraction method influences in the biochemical composition of the biomass. We also conducted a Soxhlet extraction of metabolites using three different solvents, in order to investigate the antimicrobial, antiparasitic and antioxidant capacity of Mexican Caribbean Sargassum. We found that the composition of Sargassum is influenced by season changes. Harvesting Sargassum directly from the shallow water make it more suitable for biomolecules recovery than dried Sargassum from the beach. Laccases are not suitable enzymes for Sargassum hydrolysis, and MAE had better extraction performance than EAE. We also found that Sargassum has an important antioxidant activity, and an antimicrobial effect using different solvent fractions. Finally, Sargassum present a great potential as a biosorbent of pollutants such as toxic metals in coastal ecosystems. In general, this thesis made an approach to Bio-refinery of Sargassum biomass from Mexican Caribbean coast using green technology to extract bioactive compounds, and make the most of resources that have a potential biotechnological application for biofuel, agriculture, cosmetic, nutraceutical and pharmaceutical industries.
- Development and characterization of essential oils based nanoliposomes with antifungal potentialities for biomedical applications(Instituto Tecnológico y de Estudios Superiores de Monterrey, 2020-11) Aguilar Pérez, Katya Michelle; Nasir Iqbal, Hafiz Muhammad; hermlugo/tolmquevedo; Parra Saldívar, Roberto; Escuela de Ingeniería y Ciencias; Campus Estado de México; Medina Medina, Dora IlianaThe number of cases related to superficial or systemic fungal infections has been increasing throughout the last three decades worldwide, couple with this, the current treatments to address these kind of infections are carried out during long periods and can present side effects, especially those for oral administration. The necessity to develop more efficient, biocompatible, patient compliance, and safer treatments in biomedical settings is receiving special attention using nanotechnology as a potential platform to design new drug delivery systems (DDS). Despite the broad range of novel nanocarrier systems in drug delivery, lack of biocompatibility, poor penetration, low entrapment efficiency, and toxicity are some of the significant challenges that remain to address. Such practices are even more demanding when bioactive agents are intended to be loaded on a nanocarrier system, especially for topical treatment purposes. For the previous reasons, the search for more efficient nano-vesicular systems with a high biocompatibility index and controlled releases. With particular reference, nanoliposomes, as robust nanocarriers, are becoming popular for drug delivery applications because of safety, patient compliance, and quick action. Nanoliposomes are bilayer vesicles at the nano scale having an aqueous core and one or more concentric phospholipid membranes able to encapsulate hydrophilic and hydrophobic compounds, they can be synthetized by using natural ingredients (e.g., egg yolk and soybean lecithin) which allow them to act as effective delivery drug system. Among the numerous replacements plant-derived compounds, essential oils (EOs) have revealed a wide range of biological activities, including inhibition against fungi species. However, their implementation as antifungal agents has been limited due to their volatility, low water solubility and limited administration routes. In the present work it was developed the synthesis and characterization of three different essential oils: clove essential oil (CEO), oregano essential oil (OEO) and tea tree oil (TTO) loaded nanoliposomes i) to explore the synthesis conditions of nanoliposomes ii) to compare the effectiveness of EOs and EOs encapsulated in nanoliposomes and iii) to investigate the antifungal efficacy against dermatophyte fungi Trichophyton rubrum by performing the Mycelial Growth Inhibition test (MGI). The mean size of the nanoliposomes around 37.12± 1.23 nm, PdI of 0.377±0.007 and zeta potential values of -36.94±0.36 mV were obtained by dynamic light scattering (DLS) and spherical morphology was confirmed by scanning electron microscopy (SEM) analysis. The presence of EO into nanoliposomes was displayed by attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy. Entrapment efficiency values of 91.57 ± 2.5% were achieved for CEO nanoliposomes. In vitro antifungal activity of nanoliposomes tested against Trichophyton rubrum strains revealed that CEO nanoliposomes exhibited the highest MGI % (98.2%) at concentration of 1.5 µl/ml compared to the rest of formulations. Therefore, this work revealed that EOs loaded nanoliposomes can be used as natural antifungal agents for pharmaceutical proposes.