Ciencias Exactas y Ciencias de la Salud
Permanent URI for this collectionhttps://hdl.handle.net/11285/551039
Pertenecen a esta colección Tesis y Trabajos de grado de las Maestrías correspondientes a las Escuelas de Ingeniería y Ciencias así como a Medicina y Ciencias de la Salud.
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- Design for manufacturing and assembly of a multi material bioprinting system towards tissue engineering applications(2025-06-13) Lera Julián, Miguel Ángel; Martínez López, José Israel; emipsanchez; Vázquez Lepe, Elisa Virginia; López Botello, Omar; Chuck Hernández, Cristina; School of Engineering and Sciences; Campus MonterreyLight-based techniques have great potential in bioprinting for tissue engineering, given their inherent advantages in high spatial resolution (10–100 μm) and improved cell viability (>85%) compared to traditional extrusion-based systems. However, current apparatuses found in the state of the art are limited in usability and functionality due to legacy single-material design constraints and the early development stage of photopolymerization-based bioprinters. As tissue constructs become increasingly complex, there is a need to establish a new framework for light-based equipment tailored to specific tissue engineering applications. This work presents the development of multi-material bioprinting equipment that integrates 4K digital light projection with an automated rotating four-vat system, enabling sequential use of bioinks with distinct mechanical and biochemical properties. For this endeavor, the scalability and manufacturability of the apparatus were addressed using Function Tree analysis, Quality Function Deployment (QFD), and Design for Manufacturing and Assembly (DFM&A) principles. These tools guided the definition of a feature set for meniscal tissue regeneration, including layered constructs with stiffness gradients and bioactive cues. The system was designed in Fusion and fabricated using a combination of rapid prototyping techniques. This included the 3D printing of custom resin vats, CNC machining of structural elements, and the development of bespoke electronic components for control and actuation. Initial validation was carried out using a single-vat configuration and Anycubic clear photopolymer resin. Printing trials demonstrated the resolution capacity of the optical system and successful layer-by-layer polymerization using 405 nm light exposure. These results confirm the operational feasibility of the system and establish a baseline for future multi-material implementation using photocurable bioinks
- Manufacturing of three-dimensional micromixers using additive manufacturing and non-conventional processes(Instituto Tecnológico y de Estudios Superiores de Monterrey, 2023-12) Yáñez Espinosa, Christian Rodrigo; Martínez López, José Israel; emipsanchez; Vázquez Lepe, Elisa Virginia; García López, Erika; School of Engineering and Sciences; Campus MonterreyThis work explores strategies for manufacturing complex three-dimensional micromixers by using unconventional technologies such as stereolithography (SLA), lost-wax casting, and pyrolysis. The employment of these technologies is assessed towards the development of a new generation of methodologies enabled by advanced manufacturing that includes features to integrate micromixing on devices for medical and environmental applications. Three different technologies were studied to evaluate the potential to improve the flexibility and resolution of devices designed to stir and mix reagents within systems where mass transfer is limited by laminar flow regimes. For this work, the state-of-the-art manufacturing for micromixing devices was investigated, and then experimental assessment of the potential technologies was evaluated for a novel helicoidal micromixer design. Insights of the current state of manufacturing for microdevices were carried out using computational fluid dynamics to evaluate the potential of recent technologies compared with more conventional manufacturing technologies.
- Parametric modelling of a biomimetic propulsion system using additive manufacturing for autonomous underwater vehicles(Instituto Tecnológico y de Estudios Superiores de Monterrey, 2021-12-01) Lima Rodríguez, Biali Fernando; Martínez López, José Israel; puemcuervo; Vázquez Lepe, Elisa Virginia; Chuck Hernandez, Cristina Elizabeth; School of Engineering and Sciences; Campus MonterreyThis work presents a novel pseudorandom algorithm for generating in-silico biomimetic models of caudal fins for additive manufacturing for flexible materials. The methodology provides a tool to develop caudal fin models for different morphologies (within rounded, truncated, forked, and lunate), geometrical features and, considering randomness to improve the lifelikeness of the model. The capability of the algorithm to generate designs with customized hydrodynamic features was evaluated in-silico using computational fluid dynamics comparing the maximum velocity and the angle of attack. Numerical data shows that customization of key dimensional can be integrated into a flexible and dynamic design process. This work is a step towards reproducing more robust and lifelike engineering systems.
- Development of a Low-Cost and High-Resolution Stereolithography Bioprinting System(Instituto Tecnológico y de Estudios Superiores de Monterrey, 2021-08) Pérez Cortez, Juan Enrique; Martínez López, José Israel; emimmayorquin; Rodríguez González, Ciro Ángel; Chuck Hernández, Cristina; Escuela de Ingeniería y Ciencias; Campus Monterrey; Vázquez Lepe, Elisa VirginiaResearchers that require access to 3D biological constructs for tissue engineering face significant cost barriers when it comes to using light-based bioprinters. The bioprinters that are typically used are either commercial-grade expensive technology or modified DIY extrusion apparatuses. Despite the potential benefits of low-cost equipment, there are few examples of such equipment being used in the context of light-based bioprinters. Additionally, the high cost of bioinks, large volume consumption, and a lack of information on parameter selection for light-based bioprinting restrains the adoption of this technology. In this work, retrofitting a commercial light-based 3D printer is showcased to demonstrate that it is possible to adapt this technology to build low-cost and high-resolution 3D bioprinters. To prove the capacities of this equipment, several manufacturability and biological tests are performed with a worldwide used bioink, Gelatin Methacryloyl (GelMa). A set of experiments performed in two different equipment (Anycubic Photon Mono 2K and 4K) is documented. Moreover, in this work it is showcased the use of C2C12 and Alpha TC1 Clone 6 cells to create muscular fibers and a mini pancreas model, respectively. Actin-Dapi and Live-Dead fluorescence assays at different days of culture show positive results for biocompatibility of the process. This work demonstrate that the presented low-cost retrofitting has great potential to be used as a 3D bioprinting system