Ciencias Exactas y Ciencias de la Salud
Permanent URI for this collectionhttps://hdl.handle.net/11285/551039
Pertenecen a esta colección Tesis y Trabajos de grado de las Maestrías correspondientes a las Escuelas de Ingeniería y Ciencias así como a Medicina y Ciencias de la Salud.
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- In vitro evaluation of delivery systems integrating huauzontle (Chenopodium berlandieri) extracts with anti-inflammatory and antioxidant properties for their potential use in the cosmetic industry(Instituto Tecnológico y de Estudios Superiores de Monterrey, 2022-05-25) Mejía Valdez, Daniel Eduardo; Guajardo Flores, Daniel; puemcuervo, emimayorquin; Antunes Ricardo, Marilena; School of Engineering and Sciences; Campus Monterrey; Martínez Ávila, MarianaChenopodium berlandieri spp. (Huauzontle) is a Mexico-native pseudocereal with interesting compounds. It is considered a traditional food crop since prehispanic times, and it is still consumed as a fresh or cooked vegetable. Its enhanced nutritional properties of bioactive compounds, such as saponins and oleanolic acid, are giving it the attention of scientists. Here, we propose the development of a liposome delivery system integrating an extract and a hydrolyzed extract from huauzontle for their potential use in the cosmetic industry. Conventional solid/liquid extraction will be performed to obtain saponin-rich extracts, and through HPLC-ELSD and LC-MS/TOF, their characterization will be carried out. Once characterized, the extract will be hydrolyzed in order to obtain oleanolic acid, which is also intended to be integrated into a liposome delivery system. Finally, to measure the efficacy of the liposomes, the biological activities of dermatological importance for androgenic alopecia, acne vulgaris, and skin wrinkling will be tested. We expect to observe particle sizes of the liposome delivery system below 200 nm, necessary for a correct permeability of the skin; additionally, we expect to achieve good biological activities to prevent and treat stated skin conditions; those expected results are crucial to deem the introduction of these delivery system loaded with huauzontle extracts for their potential use in the cosmeceutical industry.
- Encapsulation of isorhamnetin glycosides from opuntia ficus-indica through a liposomal delivery system and its anti-aging effect(Instituto Tecnológico y de Estudios Superiores de Monterrey, 2021-06-15) Figueroa Robles, Alejandra; GUAJARDO FLORES, DANIEL; 256061; Guajardo Flores, Daniel; puelquio/mscuervo; Martínez Avila, Mariana; School of Engineering and Sciences; Campus Monterrey; Antunes Ricardo, MarilenaIntroduction. Opuntia ficus-indica, known as prickly pear, has multiple biological applications, many of them attributed to its phenolic compounds: isorhamnetin glycosides. In addition to having these advantages, these compounds have limitations characterized by their high polarity that limits their permeability through skin layers for use in topical formulations, bioavailability due to the size of the structures, the rapid metabolization and thermosensitive feature. In order to increase their stability and solubility, the compounds were encapsulated through a liposomal delivery system. Methods. The extraction of the compound was carried out by means of ethanolic extraction in a 50:50 ethanol:water ratio. The isorhamnetin glycosides were identified by a high pressure liquid chromatograph equipped with a photodiode array detector and quantified as milligrams of total isorhamnetin glycosides per extract gram by a high-pressure liquid chromatograph equipped with a UV-Vis detector. The liposomes were synthesized by the thin-film layer evaporation and extrusion technique through a mixture of lipids (phosphatidylcholine and cholesterol in the following ratios: 7:3 and 10:1) using chloroform and methanol as solvents. Diameter size, polydispersity index, zeta potential, and stability studies were evaluated through dynamic light scattering (DLS) measurements in a zetasizer instrument. Encapsulation efficiency and release percentage analysis were also evaluated through a high-pressure liquid chromatograph equipped with a UV-Vis detector. Results. Liposomes of nanometric size were obtained consisting of a combination of cholesterol and phosphatidylcholine (70:30) ranging from 77.68 ± 10.76 nm to 297.20 ± 33.79 nm. The encapsulation efficiency of the liposomes is on a range from 87.85% ± 13.49 to 98.61% ± 0.93. More stable smaller particle size values were achieved when using a 7:3 lipid ratio in the liposomal synthesis. Also, these liposomes demonstrated an anti-elastase activity of 21.72% and a 15.83% of anti-collagenase activity. After the 72 h isorhamnetin glycosides release analysis, the results indicate around a 50% release rate. Conclusions. Encapsulation of isorhamnetin by liposomes improves the stability and solubility of the compounds, making it a more effective delivery system. The usage of a cosmetic formulation including isorhamnetin glycosides loaded liposomes can prevent skin aging based on a controlled delivery system technology. This product will be preferred by customers which tend to buy natural cosmetic products.