Ciencias Exactas y Ciencias de la Salud
Permanent URI for this collectionhttps://hdl.handle.net/11285/551039
Pertenecen a esta colección Tesis y Trabajos de grado de las Maestrías correspondientes a las Escuelas de Ingeniería y Ciencias así como a Medicina y Ciencias de la Salud.
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- Optimization in the isolation and purification procedure of pristimerin, an anticancerigen, by Aqueous Two-phase Systems(Instituto Tecnológico y de Estudios Superiores de Monterrey, 2021-06-11) González Félix, Martha Alicia; González Valdez, José Guillermo; puemcuervo; Ibarra Herrera, Celeste Concepción; González Torres, Maykel; School of Engineering and Sciences; Campus Monterrey; Mejía Manzano, Luis AlbertoThe natural triterpene pristimerin possesses interesting potential against several types of cancer. However, its production at industrial levels requires novel environmentally friendly and efficient extraction procedures. Here we analyze an ethanol-phosphate Aqueous Two-Phase Systems (ATPS) and its design parameters such as Tie-Line Length (TLL) and volume ratio (VR) on the direct extraction of pristimerin, concluding that high TLL 70 % (w/w) and a low VR (0.33) values provide the best extraction yield and purity. Partition experiments provided a compound KP value of 3.12 ± 1.21, reaffirming its preference for the ethanolic-rich phase. Besides, green assessment positioned the extraction in the classification A with 90.93 of 100 points. Furthermore, analysis at different laboratory scales (1X, 10X and 100X) demonstrated the scalability and robustness. Finally, a complete bioprocess based on the obtained results is suggested as a tool to address the pharmaceutical potential of this molecule in a sustainable and efficient manner.
- Exosome-mediated insulin delivery for the treatment of diabetes mellitus(Instituto Tecnológico y de Estudios Superiores de Monterrey, 2020-11-16) Rodríguez Morales, Belén; GONZALEZ VALDEZ, JOSE GUILLERMO; 234501; González Valdez, José Guillermo; tolmquevedo, emipsanchez; Hernández Pérez, Jesús; Rodríguez Sánchez, Dariana Graciela; School of Engineering and Sciences; Campus Monterrey; Antunes Ricardo, MarilenaExosomes are membrane-bound extracellular nanovesicles of 30-150 nm released by almost all cell types. Since they were discovered in 1983, numerous studies yielded clear evidences that exosomes serve as essential messengers for hundreds of biological-signaling processes as well as in pathological processes. In consequence of the discovery of exosomal natural functions, a wide range of applications have been developed in several fields of study, especially in drug delivery for therapeutic use. Following this growing stream of study, the aim of this work was to test the efficiency of exosome-mediated human insulin delivery using exosomes extracted from three different cell lines: hepatocellular carcinoma (HepG2); primary dermal fibroblasts (HDFa) and pancreatic β cells (RIN-m); all related to the production and/or the ability to sense insulin and, consequently regulate glucose levels in the extracellular medium. The obtained results revealed that the optimal loading efficiency was achieved by a 200 V electroporation, in comparison to an incubation at room temperature. Moreover, the maximum in vitro exosome uptake was reached after 6 h of incubation with loaded exosomes, and lightly decrease 24 h after adding the exosomes. Furthermore, glucose quantification assays revealed that exosome-mediated incorporation of insulin present significative differences in HDFa and HepG2 cells, enhancing the transport in HDFa, in comparison with free human insulin effect in the regulation of extracellular glucose levels. On the other hand, no significative differences were found between both treatments in RIN-cells. Hence, the results suggest that exosomes could be a potentially valuable tool for a biocompatible and stable insulin delivery as part of a future treatment of diabetes mellitus.