Ciencias Exactas y Ciencias de la Salud
Permanent URI for this collectionhttps://hdl.handle.net/11285/551039
Pertenecen a esta colección Tesis y Trabajos de grado de las Maestrías correspondientes a las Escuelas de Ingeniería y Ciencias así como a Medicina y Ciencias de la Salud.
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- PVA /alginate/AgNPs/curcumin electrospun nanofibers-based nonwoven mat as wound dressing(Instituto Tecnológico y de Estudios Superiores de Monterrey, 2025-06-13) Rodríguez Lee, Fernando Arturo; García López, Erika; emipsanchez; Rodríguez González, Ciro Ángel; Tejeda Alejandre, Raquel; School of Engineering and Sciences; Campus Monterrey; Vázquez Lepe, Elisa VirginiaWound healing requires advanced solutions that can support tissue regeneration, reduce inflammation, and prevent infections. This thesis presents the development of an electrospun wound dressing made from polyvinyl alcohol (PVA), alginate, silver nanoparticles (AgNPs), and curcumin. The objective was to create a multifunctional dressing capable of improving healing in burn wounds by combining the mechanical stability of PVA, the moisture retention of alginate, and the antimicrobial and anti-inflammatory properties of AgNPs and curcumin. Rheological tests were performed to identify critical polymer concentrations and determine the influence of molecular weight on fiber morphology. Electrospinning conditions were optimized to obtain bead-free nanofibers with diameters ranging from 200 to 600 nm. Scanning electron microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDS) confirmed the presence and distribution of active agents in the fibers. Biological evaluation using fibroblast 3T3 cells under direct contact conditions demonstrated good cell compatibility, especially in the first three days. It is important to highlight that by day seven, the culture medium was not replaced, yet cells still showed metabolic activity despite the reduced nutrients and suboptimal pH. This result suggests that the material provides a suitable environment that supports cell viability over time. The results show that this electrospun dressing has potential for use in burn wound healing applications.
- 3D printed emitters for nanofibers production using VAT photopolymerization(Instituto Tecnológico y de Estudios Superiores de Monterrey, 2023-06-16) Almanza Vázquez, Luis Enrique; García López, Erika; dnbsrp; Rodríguez Gónzalez, Ciro Ángel; School of Engineering and Science; Campus Monterrey; Tejeda Alejandre, RaquelElectrospinning is a method centered on electrostatic forces for fabricating continuous nanofibers with a substantial active surface area per mass unit in which the morphology of electro spun nanofiber is influenced by some parameters such as voltage, space electric field distribution, surface charge density, liquid supply rate, solution surface tension, viscosity, conductivity, and humidity. This technique is being applied by designing a Multiplexed Source using VAT photopolymerization which will allow to produce the nanofibers. Several test probes varying geometrically (Hexagonal, Pentagonal, Quadrangular, Triangular and Circular) were designed to analyze the resolution of the EnvisionTEC and to observe if the multiplexed source geometry nozzles should be changed or remain the same. After an Error Data Analysis, it was concluded that the circular geometry was the one to work with because it does not get clogged by the time the experiments were computed thus because it does not have any angle or angles that affects its manufacturing in comparison with the other geometries. In this study the polymer used to produce the nanofibers was polyethylene oxide (PEO) with a molecular wight of 900,000 gr/mol. The electrospinning experiments were conducted at flow rates ranging from 0.1 and 1 ml / hr and working distances between 12.5 and 16.5 cm. The voltage remains constant at the value of 20kV. The collected fibers were analyzed using Scanning Electron Microscopy (SEM). Based on the solution and processing conditions, different structures from droplets, and heavily beaded fibers to defect-free mats were obtained. PEO’s concentration was 4% w/v and the volume of Deionized water was 96 ml. The solution was diluted and prepared by using a water bath for 8 hours until the PEO is completely diluted. Based on the solution and processing conditions, different structures from droplets, and heavily beaded fibers to defect-free mats were obtained and measured their diameter (if applicable) by using the image j software and by computing a Data analysis of the average diameter per sample of nanofibers check out which one is the optimal combination of parameters by producing the nanofibers. The final application of this is the manufacture of a multilayer patch for a biomedical application that is the tissue regeneration for second degree burning wounds.