Ciencias Exactas y Ciencias de la Salud
Permanent URI for this collectionhttps://hdl.handle.net/11285/551039
Pertenecen a esta colección Tesis y Trabajos de grado de las Maestrías correspondientes a las Escuelas de Ingeniería y Ciencias así como a Medicina y Ciencias de la Salud.
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- Design and characterization of an adhesive and pH-indicating hydrogel with gentamicin release as a proof of concept for its potential use as an acute wound dressing(Instituto Tecnológico y de Estudios Superiores de Monterrey, 2021-12-06) Viaña Mendieta, Pamela; Benavides Lozano, Jorge Alejandro; puemcuervo; Antunes Ricardo, Marilena; Mata Gómez, Marco Arnulfo; School of Engineering and Sciences; Campus Monterrey; Sánchez, Mirna LorenaWound care cost is an overwhelming problem aggravated by the burning of skin injuries and the overuse of traditional materials that are low cost-efficient. Modern wound care strives for multifunctional wound dressings to monitor physiological conditions and prevent wound infection or non-healing processes. Thus, this study addresses the synthesis and characterization of an adhesive and pH-indicating hydrogel dressing with gentamicin release for the potential acceleration and monitoring of acute wounds. The proposed hydrogels were prepared by physical and chemical crosslinking of polyvinyl alcohol (PVA), phenol red, glycerol, citric acid (CA), butyl-cyanoacrylate (BCA), and gentamicin. Diverse formulations, varying PVA and CA concentration, were evaluated and selected by the stable swelling profile. Then, the selected formulation was characterized by swelling degree, colorimetric pH-change evaluations, adhesion and dehydration assay, and drug release profile. The selected formulation was 4% wt. PVA, 5% wt. CA, 10% wt. glycerol and 1.5% wt. BCA, and showed excellent properties as a wound dressing. It had uniform transparency, a good pH-indicating property, enough water content, stable swelling degree and gentamicin release. Thus, the pH-indicating property showed the transition of pH 4 to 9, with sensitive pH change by forming the circle-like pattern at pH 8 and 9. The dehydration ratio was 1.0 and maintained adhesion to a plastic surface for 96 h. The release of gentamicin was enough in 24 h (96.3 %). Based on the results, this proof of concept concludes that the designed multifunctional hydrogel has suitable properties as a potential wound dressing. This study shows the opportunity to continue its characterization in detail in searching for a potential commercial wound dressing.
- Effects of acetaminophen, diclofenac and ibuprofen exposure on growth, viability, photosynthetic pigment production and gene expression in Synechococcus elongatus for the assessment of its bioremediation potential(Instituto Tecnológico y de Estudios Superiores de Monterrey, 2020-12-18) Castillo Escalante, Francisco Adrián; Benavides Lozano, Jorge Alejandro; tolmquevedo; González Valdéz, José Guillermo; Santacruz López, Yolanda Arlette; Escuela de Ingeniería y Ciencias; Campus Monterrey; Hernandez Pérez, JesúsThe extensive use of pharmaceuticals has led to the accumulation of emergent pollutants with potential toxicity to aquatic organisms in water bodies around the world. Non-steroidal anti-inflammatory drugs (NSAIDs), such as acetaminophen (ACP), diclofenac (DCF) and ibuprofen (IBU) are amongst the most prevalent pharmaceuticals found in surface water and wastewater at levels up to 10,000 ng/L. In the present work, a Mexican strain of Synechococcus elongatus was selected to characterize the effects of ACP, DCF and IBU on the cyanobacteria growth rate, viability, photosynthetic pigment content (chlorophyll, carotenoids and phycobiliproteins), and expression level of three stress-related genes (aphC, htpG, and mutM). S. elongatus was able to tolerate continued exposure to ACP and DCF with 2.43% and 12.40% inhibition to its growth rate under ACP and DCF, respectively; exposure to IBU led to complete growth inhibition. Cultures treated with ACP and DCF did not show significant change in chlorophyll and phycobiliprotein content but did show a statistically significant increase in carotenoids (13.90% under ACP, p < 0.05) after 10 days of culture. IBU treated cultures presented total loss of photosynthetic pigments except for chlorophyll, with a 70% decrease compared to control, showing a similar state to chlorosis in cyanobacteria. RNA levels showed an increase in aphC expression under all treatments but was only statistically significant under DCF (6.29-fold increase, p < 0.05); expression of htpG and mutM was downregulated without statistical significance under all treatments. These results suggest an increase in the antioxidant defense response (aphC) against reactive oxygen species under exposure to NSAIDs. Finally, S. elongatus was not able to decrease the level of NSAIDs present in the culture. This research work presents a deeper understanding of the effect of contaminant NSAIDs over cyanobacteria and suggests a potential industrial application of S: elongatus culture under pharmaceutical-contaminated wastewater.