Ciencias Exactas y Ciencias de la Salud
Permanent URI for this collectionhttps://hdl.handle.net/11285/551039
Pertenecen a esta colección Tesis y Trabajos de grado de las Maestrías correspondientes a las Escuelas de Ingeniería y Ciencias así como a Medicina y Ciencias de la Salud.
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- Controlling aggregation of Rose Bengal with nanocages to modulate the production of Reactive Oxygen Species in the context of Photochemical Tissue Bonding(Instituto Tecnológico y de Estudios Superiores de Monterrey, 2021-06-15) Alvarez López, Constanza; Aguirre Soto, Héctor Alán; puemcuervo; Kochevar, Irene E; Heyne, Belinda; Lozano García, Omar; School of Engineering and Sciences; Campus MonterreyThe healing of wounds has always been a complicated matter because suturing, the long-standing closure method, depends on the skill of the surgeon, relies on placing a foreign body in the tissue which makes it prone to inflammation and infection, and leads to scarring and, most often, lower mechanical properties of the tissue. For instance, mortality directly attributable to surgical site infections goes up to 2.5% per year in USA. Some alternative methods for the closure and treatment of wounds and surgical incisions have been studied, where one of the newest alternatives is Photochemical Tissue Bonding (PTB). PTB involves applying a photosensitive molecule, Rose Bengal (RB), to the wounded site and then exposing it to visible light (~𝟓𝟑𝟎 𝒏𝒎). Despite its proven benefits, the reaction mechanism involved in enhanced crosslinking of collagenous tissue remains unclear. It is important that we know how RB behaves to adjust the protocol of the therapy and increase its efficiency. It is believed that this process is mediated by singlet oxygen, which leads to the production of Reactive Oxygen Species (ROS). Furthermore, it is suspected that the aggregation of RB negatively affects the photon-efficiency of RB. However, a comprehensive reaction mechanism accounting for RB aggregation and photochemistry in tissue remains elusive. In this thesis, we analyzed the aggregation of RB in phosphate buffer saline (PBS) aqueous solutions and studied the effect of its dimerization on the photobleaching and oxygen consumption kinetics. To study the thermodynamics of aggregation we utilized UV-Vis absorbance spectroscopy and DLS. It was confirmed that the dimerization constant for RB in 1X PBS is in the order of 1.1 x103 M. We confirmed that at a concentration of 1mM RB is found in a 1:1 monomer/dimer ratio. Then, we compare the extent of dimerization in the absence and presence of three nanocages: cucurbit[7]uril, β-cyclodextrin and hydroxypropyl-γ-cyclodextrin (HP-γ-CD). HP-γ-CD is the nanocage that is best suited to bind to RB. Host-guest complexation of RB with HP-γ-CD appears to delay dimerization of RB in 1X PBS significantly. We then correlated aggregation to photochemical kinetics. Steady-state kinetics were tested with real-time absorbance spectroscopy and fluorescence-based oxygen monitoring. Evidence was obtained indicating that the complexation of RB with HP-γ-CD can increase the rate of consumption of molecular oxygen under irradiation. We therefore conclude that is appears possible to use an appropriate nanocage to form host-guest complexes that delay dimerization of RB is aqueous PBS solutions, and that the caging of certain moieties in the solvated RB may enhance de consumption of molecular oxygen.
- Lab-scale modular platform to study coiled-flow inverters (CFI) as candidates for continuous-flow photoreactor units: A case study based on the oxidative degradation of fluorescein induced by visible light in the presence of ZnO-APTMS-Au micro/nano-particles in aqueous suspension(Instituto Tecnológico y de Estudios Superiores de Monterrey, 2020-12-04) Tiwari, Chinmay Pramodkumar; Aguirre Soto, Héctor Alán; tolmquevedo; Gallo Villanueva, Roberto Carlos; Contreras Cruz, David Atahualpa; López Guajardo, Enrique Alfonso; School of Engineering and Sciences; Campus MonterreyVisible light-driven continuous-flow photochemistry has gained widespread recognition lately and is employed in many innovatively designed photoreactors. Out of the two main categories, slurry reactors are found to have a better reputation in terms of achieving competitive photon efficiencies when compared to immobilized catalyst type reactor designs. However, several obstacles had stalled the broad-scale implementation of this beneficial process. A few of the main imminent challenges include combating light attenuation by better mixing in continuous-flow of the suspension to allow the use of the higher photocatalyst content and require lower photon consumption. Also, the difficulties in the fabrication of intricate glass-based photoreactor designs are one of the significant challenges. An inherently better-designed reactor which deals with the common problems of conventional photoreactors is required. This thesis presents a flexible platform to study photoreactors, where a coiled flow inverter—a well-established static mixer design— is used as a micro/milli-fluidic device. The CFI is incorporated as a photoreactor for the first time for a continuous flow photodegradation study of an organic model pollutant, fluorescein, with ZnO catalyst functionalized with APTMS and Au nanoparticles to make it visible-light absorptive. Flow inversions leading to chaotic advection occurring in the CFI combats light attenuation. Due to superlative mixing coupled with a highly efficient visible light source, our photo-CFI stands to be in top slurry reactor designs as per the recently established PSTY benchmark, valued at 2.97×10^−2 (m^3 treated water day^-1 m^-3 reactor kW^-1). A brief study on the uni- and multi-axial light arrangement for complex geometries was used to analyze the effect of geometry/lighting arrangement and ensure uniform irradiation of the photo-CFI. A discussion of dye-degradation products surface interaction with photocatalyst was carried out to analyze possible explanations for an observed destabilization of the suspension during reaction, leading to depositions in the reactor. SLA based additive manufacturing is tested and projected to be a superior alternative for rapid prototyping of intricate transparent photoreactor designs in lieu of conventional glass blowing techniques of complex geometries such as those required for static mixers like the photo-CFI.
- Search for High-Molecular Weight Linear Polymers for the Formulation of Novel Solutions for the Fabrication of Micro-Fibers by Electromechanical Spinning(Instituto Tecnológico y de Estudios Superiores de Monterrey, 2020-12-02) Katagiri Tanaka, Antonio Osamu; AGUIRRE SOTO, HECTOR ALAN; 322598; Aguirre Soto, Héctor Alán; tolmquevedo, emipsanchez; Bonilla Ríos, Jaime; School of Engineering and Sciences; Campus Estado de México; Medina Medina, Dora IlianaCarbon nano-wires are versatile structures composed of carbon chains with a wide range of applications due to their high chemical resistance and electric properties. Regardless of the high interest in the implementation of carbon nano-wires in energy, environmental and health-care applications, no feasible processes have been developed to fabricate carbon nano-wires with spatial control at a reasonable cost. Carbon nano-wires have been fabricated with the use of a photoresist, but little is known about polymers that can produce conductive carbon nano-wires after pyrolysis. Various polymer solutions have been tested in near field electrospinning (NFES) and photopolymerization separately, however, few have been tested for nano-wire fabrication purposes through the process of spatio-temporal deposition with NFES, photo-polymerization for cross-linking and pyrolysis. The intention behind the thesis proposal is to use rheological analyses of different polymer solutions to determine if they can be easily electrospun at low voltages and then fabricate nano-wires with them. This thesis work arises from the need to test a greater variety of polymer-solvent combinations with the goal of designing a polymer solution to fabricate carbon nano-wires with higher conductivity than the current SU-8 polymeric nano-fibers. The present work includes the design of polymer solutions that can be electrospun, with the hope that the selected high molecular weight polymers can be photo-polymerized, and then pyrolyzed into conducting carbon nanowires. The overarching goal is to contribute towards the development of novel designed polymer solutions to achieve mass scale manufacturing of conductive carbon nano-wires in an inexpensive, continuous, simple and reproducible manner as central components for nano-sensors.