2018-10-182018-10-181932620310.1371/journal.pone.0126571http://hdl.handle.net/11285/630453Noroviruses are recognized worldwide as the principal cause of acute, non-bacterial gastroenteritis, resulting in 19-21 million cases of disease every year in the United States. Noroviruses have a very low infectious dose, a short incubation period, high resistance to traditional disinfection techniques and multiple modes of transmission, making early, point-of-care detection essential for controlling the spread of the disease. The traditional diagnostic tools, electron microscopy, RT-PCR and ELISA require sophisticated and expensive instrumentation, and are considered too laborious and slow to be useful during severe outbreaks. In this paper we describe the development of a new, rapid and sensitive lateral-flow assay using labeled phage particles for the detection of the prototypical norovirus GI.1 (Norwalk), with a limit of detection of 107 virus-like particles per mL, one hundredfold lower than a conventional gold nanoparticle lateral-flow assay using the same antibody pair. © 2015 Hagström et al.info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/4.0gold nanoparticlenanoparticlephage nanoparticle reporterunclassified drugnanoparticleantigen bindingArticleassaycontrolled studydiagnostic test accuracy studyenzyme linked immunosorbent assayintermethod comparisonlateral flow assaylimit of detectionnonhumanNorwalk virussensitivity and specificityvirus detectionvirus like agentbacteriophagebioassayCaliciviridae InfectionsEscherichia coligastroenteritisgeneticshumanisolation and purificationmetabolismNorovirusproceduresUnited StatesvirologyBacteria (microorganisms)NorovirusBacteriophagesBiological AssayCaliciviridae InfectionsEscherichia coliGastroenteritisHumansNanoparticlesNorovirusSensitivity and SpecificityUnited States7 INGENIERÍA Y TECNOLOGÍAArtículo105