García Rivas, Gerardo de JesúsSalazar Ramírez, Felipe de Jesús2021-10-042021-10-042020-05-222020-05-22Salazar-Ramírez F. (2020) The role of the mitochondrial calcium uniporter in the process of arrhythmogenesis in a murine model of acute catecholamine overload (Tesis Maestría) Instituto Tecnológico y de Estudios Superiores de Monterrey. Recuperado de: https://hdl.handle.net/11285/639909https://hdl.handle.net/11285/639909https://orcid.org/0000-0003-4731-3293Sudden cardiac death by fulminant ventricular arrhythmias remains a concern in population with cardiac risk. Recently, the mitochondrion has been implied to be a central player in Ca2+ mishandling, with its dysfunction leading up to arrhythmogenesis. A possible starting event that could lead to most changes seen in cardiac disfunction is mitochondrial Ca2+ overload. The following research study focuses on demonstrating the effects of mitochondrial Ca2+ influx inhibition in arrhythmogenesis. A murine model of acute catecholamine (isoproterenol) overload was treated previously with mitochondrial Ca2+ transport inhibitor Ru360. Ru360 treated mice showed a complete abolishment of ventricular tachycardia and ventricular fibrillation. To characterize the possible mechanisms of action, heart mitochondria were isolated and mitochondrial function was assessed. Mitochondrial Ca2+ transport inhibition preserved mitochondrial function and membrane integrity as demonstrated by a higher respiratory control and calcium retention capacity when compared to isoproterenol-treated mice which appears to be caused by a reduced oxidative stress as a trend to preserve reduced thiol groups was shown. Given the positive results obtained in abolishing ventricular arrhythmias by inhibiting mitochondrial Ca2+ transport, it is precise to continue the characterization of the mechanisms by which this therapy exerts its effects. To fully demonstrate its efficacy and characterize its mechanism of action may lead up to a new therapeutic target and therapy that could set the bases to clinical research in the near future.TextoengopenAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0MEDICINA Y CIENCIAS DE LA SALUD::CIENCIAS MÉDICAS::FISIOLOGÍA HUMANA::FISIOLOGÍA CARDIOVASCULARMedicineTesis de maestríahttps://orcid.org/0000-0001-5136-0063MCUMitochondriaArrhythmiaMitochondrial Calcium UniporterCalciumMitochondrial dysfunction876167