2018-10-182018-10-181932620310.1371/journal.pone.0187907http://hdl.handle.net/11285/630599Background and aims: ADSCs transplantation had been shown in some experimental models of kidney damage that it improves kidney function and reduces fibrosis. In this study we evaluated the effect of human adipose tissue-derived stem cell (hADSC) therapy in a chronic kidney damage experimental model. Methods A chronic kidney injury was induced by daily orogastric administration of adenine (100mg/ kg) to male Wistar rats for 28 days. hADSCs were isolated, expanded and characterized before transplantation. hADSC administration was performed in a tail vein at a dose of 2 x106 cells/animal. Animals were sacrificed at 7 days post-treatment. The percentage of fibrotic tissue, serum and urine levels of urea, creatinine, total protein and renal mRNA of COL1A1, TGFB1, CTGF, ACTA2, IL6, IL10, TNF were analyzed. Results hADSCs treatment significantly reduces kidney fibrosis, improves urea and creatinine serum and urine levels, and diminishes COL1A1, TGFB1, CTGF, ACTA2 mRNA kidney levels. Conclusions These results showed that cell therapy using hADSCs improves renal function and reduces fibrosis. © 2017 Rivera-Valdés et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/4.0ACTA2 proteinadeninealpha smooth muscle actinCOL1A1 proteincollagenconnective tissue growth factorcreatininemessenger RNAtransforming growth factor beta1unclassified drugureaadenineadipose derived stem cellanimal experimentanimal modelanimal tissueArticlecontrolled studycreatinine blood levelcreatinine urine levelhumanhuman cellkidney fibrosismalenonhumanratstem cell transplantationtreatment outcomeurea blood leveladipose tissueanimalcell culturechemically inducedcytologyfibrosisgene expression profilinggeneticskidney diseasestem cellWistar ratAdenineAdipose TissueAnimalsCells, CulturedFibrosisGene Expression ProfilingHumansKidney DiseasesMaleRatsRats, WistarStem Cells7 INGENIERÍA Y TECNOLOGÍAArtículo1212