Sensitive detection of norovirus using phage nanoparticle reporters in lateral-flow assay

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dc.creatorRichard Willson
dc.date2015
dc.date.accessioned2018-10-18T21:22:00Z
dc.date.available2018-10-18T21:22:00Z
dc.descriptionNoroviruses are recognized worldwide as the principal cause of acute, non-bacterial gastroenteritis, resulting in 19-21 million cases of disease every year in the United States. Noroviruses have a very low infectious dose, a short incubation period, high resistance to traditional disinfection techniques and multiple modes of transmission, making early, point-of-care detection essential for controlling the spread of the disease. The traditional diagnostic tools, electron microscopy, RT-PCR and ELISA require sophisticated and expensive instrumentation, and are considered too laborious and slow to be useful during severe outbreaks. In this paper we describe the development of a new, rapid and sensitive lateral-flow assay using labeled phage particles for the detection of the prototypical norovirus GI.1 (Norwalk), with a limit of detection of 107 virus-like particles per mL, one hundredfold lower than a conventional gold nanoparticle lateral-flow assay using the same antibody pair. © 2015 Hagström et al.
dc.identifier.doi10.1371/journal.pone.0126571
dc.identifier.issn19326203
dc.identifier.issue5
dc.identifier.urihttp://hdl.handle.net/11285/630453
dc.identifier.volume10
dc.languageeng
dc.publisherPublic Library of Science
dc.relationhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-84929377736&doi=10.1371%2fjournal.pone.0126571&partnerID=40&md5=615a77665329f810f1045846a97e6e70
dc.relationInvestigadores
dc.relationEstudiantes
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0
dc.sourcePLoS ONE
dc.subjectgold nanoparticle
dc.subjectnanoparticle
dc.subjectphage nanoparticle reporter
dc.subjectunclassified drug
dc.subjectnanoparticle
dc.subjectantigen binding
dc.subjectArticle
dc.subjectassay
dc.subjectcontrolled study
dc.subjectdiagnostic test accuracy study
dc.subjectenzyme linked immunosorbent assay
dc.subjectintermethod comparison
dc.subjectlateral flow assay
dc.subjectlimit of detection
dc.subjectnonhuman
dc.subjectNorwalk virus
dc.subjectsensitivity and specificity
dc.subjectvirus detection
dc.subjectvirus like agent
dc.subjectbacteriophage
dc.subjectbioassay
dc.subjectCaliciviridae Infections
dc.subjectEscherichia coli
dc.subjectgastroenteritis
dc.subjectgenetics
dc.subjecthuman
dc.subjectisolation and purification
dc.subjectmetabolism
dc.subjectNorovirus
dc.subjectprocedures
dc.subjectUnited States
dc.subjectvirology
dc.subjectBacteria (microorganisms)
dc.subjectNorovirus
dc.subjectBacteriophages
dc.subjectBiological Assay
dc.subjectCaliciviridae Infections
dc.subjectEscherichia coli
dc.subjectGastroenteritis
dc.subjectHumans
dc.subjectNanoparticles
dc.subjectNorovirus
dc.subjectSensitivity and Specificity
dc.subjectUnited States
dc.subject.classification7 INGENIERÍA Y TECNOLOGÍA
dc.titleSensitive detection of norovirus using phage nanoparticle reporters in lateral-flow assay
dc.typeArtículo
refterms.dateFOA2018-10-18T21:22:00Z

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