Reproducibility of fluorescent expression from engineered biological constructs in E. coli

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dc.creatorCinthya Cadenas
dc.date2016
dc.date.accessioned2018-10-18T20:12:50Z
dc.date.available2018-10-18T20:12:50Z
dc.descriptionWe present results of the first large-scale interlaboratory study carried out in synthetic biology, as part of the 2014 and 2015 International Genetically Engineered Machine (iGEM) competitions. Participants at 88 institutions around the world measured fluorescence from three engineered constitutive constructs in E. coli. Few participants were able to measure absolute fluorescence, so data was analyzed in terms of ratios. Precision was strongly related to fluorescent strength, ranging from 1.54-fold standard deviation for the ratio between strong promoters to 5.75-fold for the ratio between the strongest and weakest promoter, and while host strain did not affect expression ratios, choice of instrument did. This result shows that high quantitative precision and reproducibility of results is possible, while at the same time indicating areas needing improved laboratory practices. © 2016 Beal et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
dc.identifier.doi10.1371/journal.pone.0150182
dc.identifier.issn19326203
dc.identifier.issue3
dc.identifier.urihttp://hdl.handle.net/11285/630306
dc.identifier.volume11
dc.languageeng
dc.publisherPublic Library of Science
dc.relationhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-84961783220&doi=10.1371%2fjournal.pone.0150182&partnerID=40&md5=67032bb57a513e3c816ee7da868aa8bb
dc.relationInvestigadores
dc.relationEstudiantes
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0
dc.sourcePLoS ONE
dc.subjectDNA structure
dc.subjectgene expression regulation
dc.subjectpromoter region
dc.subjectreproducibility
dc.subjectbiosynthesis
dc.subjectEscherichia coli
dc.subjectgenetic transcription
dc.subjectgenetics
dc.subjectgood laboratory practice
dc.subjectmetabolism
dc.subjectprotein engineering
dc.subjectreproducibility
dc.subjecttranscription initiation
dc.subjectgreen fluorescent protein
dc.subjectEscherichia coli
dc.subjectGreen Fluorescent Proteins
dc.subjectLaboratory Proficiency Testing
dc.subjectPromoter Regions, Genetic
dc.subjectProtein Engineering
dc.subjectReproducibility of Results
dc.subjectTranscription, Genetic
dc.subjectTranscriptional Activation
dc.subject.classification7 INGENIERÍA Y TECNOLOGÍA
dc.titleReproducibility of fluorescent expression from engineered biological constructs in E. coli
dc.typeArtículo
refterms.dateFOA2018-10-18T20:12:50Z

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