Ciencias Exactas y Ciencias de la Salud
Permanent URI for this collectionhttps://hdl.handle.net/11285/551014
Pertenecen a esta colección Tesis y Trabajos de grado de los Doctorados correspondientes a las Escuelas de Ingeniería y Ciencias así como a Medicina y Ciencias de la Salud.
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- Multistage continuous reactors system as a strategy for the comprehensive intensification of prodigiosin production by extractive fermentation(Instituto Tecnológico y de Estudios Superiores de Monterrey, 2024) Salas Villalobos, Ulises Andres; AGUILAR JIMENEZ, OSCAR ALEJANDRO; 278440; emimmayorquin; Torres Acosta, Mario Antonio; Sukumara, Sumesh; Sudarsan, Suresh; Santacruz Lopez, Yolanda Arlette; Escuela de Ingenieria y Ciencias; Campus MonterreyThe increasing demand for bioproducts has prompted significant advancements in production technologies, addressing both technical complexities and economic/environmental constraints. Multistage continuous reactors (MSCR) have emerged as a promising approach for the intensified production of valuable secondary metabolites like prodigiosin. This study explores the integration of MSCR with in-situ product recovery (ISPR) and economic-environmental analysis to optimize prodigiosin production. Nutritional optimization revealed that an optimal C:N ratio of 8:1 using peanut and defatted soybean meal significantly increased prodigiosin production. Coupling ISPR with mineral oil facilitated efficient prodigiosin extraction, alleviating product inhibition and concentrating the product, thus reducing downstream processing size and waste streams. Our research employed a factorial design to assess the impact of dilution rate and reactor arrangement on prodigiosin yield, achieving a maximum concentration of 710.61 mg L^-1 and a biomass of 5.1x10^9 CFU mL^-1. This multistage approach enhanced productivity by compartmentalizing growth and stress phases, allowing for higher production per cell and system flexibility. Economic assessment indicated a 45% and 20% cost reduction compared to batch and single continuous fermentation, respectively, with labor and materials as key cost factors. The developed optimization factor methodology identified a dilution rate of 0.0248 h^-1 as optimal for minimizing production costs and environmental impact. The integration of MSCR, ISPR, and economic-environmental analysis provides a transformative approach for bioprocess intensification. By optimizing reaction stages and continuously recovering products, this strategy significantly improves efficiency and yield. The holistic consideration of economic and environmental factors ensures sustainable and economically viable industrial practices, advancing the commercialization of innovative biotechnological solutions.