Ciencias Exactas y Ciencias de la Salud
Permanent URI for this collectionhttps://hdl.handle.net/11285/551014
Pertenecen a esta colección Tesis y Trabajos de grado de los Doctorados correspondientes a las Escuelas de Ingeniería y Ciencias así como a Medicina y Ciencias de la Salud.
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- Development and in vivo evaluation of a probiotic-enriched functional beverage with Bacillus licheniformis for gut microbiota modulation in a high-fat diet model in C57BL/6 mice(Instituto Tecnológico y de Estudios Superiores de Monterrey, 2025-01-01) Ramírez Olea, Hugo; Chavez-Santoscoy, Rocío Alejandra; Vázquez Lepe, Elisa Virginia; mtyahinojosa; Hernández Pérez, Jesús; Yolanda Arlette Santacruz Lopez; García Cayuela, Tomas; González Soltero, María del Rocío; Escuela de Ingeniería y Ciencias; Campus MonterreyObesity, type 2 diabetes, and other metabolic diseases are increasing across the globe. The necessity for public health to discover new interventions that can aid in fighting these conditions is crucial. Obesity, overweight, and diabetes are common conditions in Mexico. Over 70% of adults are overweight. Over a third are obese. Over 14 million people have diabetes. Understanding the link between these metabolic disorders with gut microbiota dysbiosis could therefore be critical to developing novel strategies for the prevention and treatment of these conditions. This PhD thesis intends to develop a functional drink using Bacillus licheniformis to modulate the gut microbiota and subsequently metabolism in general. In this thesis, a review of B. licheniformis was performed, enhancing its probiotic properties and mechanism of action concerning metabolic disorders such as obesity and diabetes. B. licheniformis was chosen due to its probiotic properties regarding microbiome modulation and its helpful effects on metabolic disorders. A native strain was isolated, sequenced, and optimized for use as a single-strain probiotic. Microencapsulation of the probiotic by spray drying using a maltodextrin-alginate-inulin matrix was statistically optimized and yielded microcapsules that have low moisture content (3.02%) and high yield (51.06%) and encapsulation efficiency (80.53%), solubility (90.52%), and stability at 4 °C, 25 °C, and 37 °C for at least six months, maintaining probiotic viability under simulated gastrointestinal conditions. Structural analyses (SEM, DLS, Zeta potential, FTIR, XRD, and DSC) showed interactions between the bacterium and the matrix, promoting an improvement in the amorphization, thermal stability, and release profile. The encapsulated probiotic has been added to a non-dairy functional beverage and shown to be viable through storage for over 6 months. Finally, it was evaluated in a high-fat diet Model using C57BL/6 mice. This functional beverage supplementation revealed a decrease in weight gain, prevention of hepatic steatosis, and systemic inflammation. At the same time, it improved glucose tolerance, biochemical profiles, gut microbiota diversity, and gene expressions compared to unsupplemented controls. By combining microbiology, materials science, and metabolic physiology, we present Bacillus licheniformis as a viable alternative platform for functional food products to aid in the control of obesity, diabetes, and other possible metabolic diseases through gut microbiota modulation.
- Exosome-like vesicles in intercellular communication: Investigating the role of exosomal proteins in the pathophysiology of obesity and exploring the potential therapeutic use of exosomes.(Instituto Tecnológico y de Estudios Superiores de Monterrey, 2023-06-15) Donoso Quezada, Javier Alejandro; GONZALEZ VALDEZ, JOSE GUILLERMO; 234501; González Valdez, José Guillermo; puemcuervo, emipsanchez; Ramos Parra, Perla Azucena; Genevieve Brunck, Marion Emilie; Chávez Santoscoy, Rocío Alejandra; Gómez Loredo, Alma Elizabeth; Escuela de Ingeniería y Ciencias; Campus Monterrey; Brix Pedersen, SusanneType 2 diabetes mellitus (T2DM) is a chronic condition characterized by impaired insulin sensitivity, resulting in hyperglycemia, dyslipidemia, and other metabolic changes that can damage organs and tissues over time. Obesity is the primary risk factor for the development of T2DM, as it triggers chronic inflammation in adipose tissue, leading to the secretion of adipokines that reduce insulin sensitivity in peripheral tissues. On their part, exosomes, small extracellular vesicles that cells use for intercellular communication, are critical players in fundamental biological processes such as cell growth, metabolism, and inflammation. Changes in the production or composition of exosomes can lead to health issues. In this dissertation, we explore the role of exosomes in cell-to-cell communication and their potential therapeutic use, focusing on the proteomic alterations that occur in exosomes during obesity and their potential functional consequences. The experimental work in this dissertation is divided into three parts. First, we studied the effect of hyperglycemia on cell function in adipocytic and hepatocytic cell lines. Second, we investigated the changes in exosome proteome resulting from obesity and physical training in a mouse model of diet- induced obesity, emphasizing the functional implications of these alterations. Finally, we evaluated the potential therapeutic use of exosomes to deliver bioactive compounds in vitro. This work aims to enhance our understanding of exosome biology and its relevance to health and disease, particularly metabolic disorders such as obesity and T2DM. By shedding light on the functional consequences of altered exosome proteome and exploring the potential of exosomes for therapeutic purposes, this dissertation provides important insights that may pave the way for novel therapeutic approaches for metabolic disorders.