Ciencias Exactas y Ciencias de la Salud
Permanent URI for this collectionhttps://hdl.handle.net/11285/551014
Pertenecen a esta colección Tesis y Trabajos de grado de los Doctorados correspondientes a las Escuelas de Ingeniería y Ciencias así como a Medicina y Ciencias de la Salud.
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- Cancer Therapeutics: Functionalized Magnetic Nanoparticles for Targeted Drug Delivery for the Treatment of Cancer(Instituto Tecnológico y de Estudios Superiores de Monterrey, 2023) de la Fuente Jiménez, Juan Luis; Sharma, Ashutosh; emimmayorquin; Zentella Dehesa, Alejandro; Tapia Ramírez, José; Vera Hernández, Arturo; School of Engineering and Sciences; Campus Querétaro; Oza, GoldieBreast cancer (BC) has been a terrible disease in which women had suffer due to all the emotional, financial, familiar, and healthy issues it come with. Several subtypes of BC can be treated with different medicinal approaches like hormone therapy. However, the triple negative breast cancer (TNBC) subtype is the more lethal, deathly, and with the worst survival rate due to the absence of the progesterone and estrogen receptors (PR and ER, respectively) as well as the human epidermal growth factor receptor 2 (HER2). This deficiency of receptors in TNBC patients creates a gap with the medicinal treatments since the existing ones are focused on being applied on those receptors. Therefore, new treatments are needed for assisting TNBC patients. One possible solution is found in the power of nanoparticles (NPs), especially magnetic NPs (MNPs) which can help treat TNBC patients. In the present study, zinc ferrite (ZFO) and lanthanum ferrite (LFO) MNPs were synthetized via co-precipitation method and then characterized for knowing their properties and behaviors. Several techniques were utilized for knowing the size, shape, surface and optical properties, magnetic and electron properties, and aggregation and stability. Once the characterization was completed, different cytotoxic assays were employed for knowing the potential toxicity of ZFO and LFO MNPs towards breast cancer cell lines. The occupied cell lines were breast adenocarcinoma cell (MCF-7), triple negative breast cancer (MDA-MB-231) and normal cell lines (HEK-293). The different results exhibit the MDA-MB-231 cell line as more susceptible to die once’s treated with the MNPs. vii Moreover, once the first step was completed, ZFO MNPs were coated with gold for making a more stable MNPs and then covered with folic acid (FA) and chemotherapeutic drug doxorubicin (Dox). This procedure is intended to make an active targeting of the newly ZFO nanocomposite since the breast cancer cells have an overexpression of FA receptors. In addition, the nanocomposites are characterized for knowing if gold, FA, and Dox are attached to the ZFO MNPs. Then to know if this newly formed nanocomposite can deliver the Dox in the tumor area which has a specific pH, different experiments are completed. This will clear the idea if functionalized nanocomposites can travel towards the tumor area and deliver Dox into the cancerous cells achieving a higher mortality.
- Pharmacokinetic-pharmacodynamic evaluation of a fraction with a neuroprotective activity of Tagetes lucida(Instituto Tecnológico y de Estudios Superiores de Monterrey, 2022-12-05) Santibáñez García, Guadalupe Anislada; SANTIBAÑEZ GARCIA, GUADALUPE ANISLADA; 825903; Sharma, Ashutosh; puemcuervo, emipsanchez; Angulo Bejarano, Paola Isabel; Castillo Maldonado, Irais; Herrera Ruiz, Maribel Lucila; School of Engineering and Sciences; Campus Monterrey; Jiménez Ferrer, Jesús EnriqueTagetes lucida has been widely used as a folk remedy in illnesses associated with the central nervous system (CNS) and inflammatory ailments. Among the compounds that stand out in the plant against these conditions are coumarins, such as 7-O-prenylscopoletin (PE), scoparone (SC), dimethylfraxetin (DF), herniarin (HR), and 7-O-prenylumbelliferone (PU). Therefore, the relationship between the therapeutic effect and the concentration can be evaluated through pharmacokinetic-pharmacodynamic (PK–PD) studies under a model of neuroinflammation induced by lipopolysaccharide (LPS). In the present study, a bioanalytical method was established by HPLC-UV for the simultaneous quantification of the coumarins present in the active fraction of T. lucida, which was able to determine the temporal concentration profiles of each of the coumarins in the plasma, brain, kidney, and spleen samples of healthy and damaged mice. Coumarins showed an increase in plasma concentrations of up to three times in the neuroinflammation model, compared to healthy mice, so it was possible to quantify the therapeutic agents in the main target organ, the brain. The ability of compounds to cross the blood-brain barrier is an advantage in the treatment of diseases associated with neuroinflammation processes, A pertinent assay that was proposed in the present investigation with T. lucida, is the measurement of the extravasation of the Evans blue dye in the brain of mice, with and without LPS, that causes neuroinflammation and an increase in the permeability of the blood-brain barrier. Future PK-PD assessments at different doses and longer test times are proposed to obtain parameters that determine the best possible effect without toxic responses.